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Production of EGF-containing polypeptides in Xenopus oocytes microinjected with submaxillary gland mRNA.
Burmeister M
,
Avivi A
,
Schlessinger J
,
Soreq H
.
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The biosynthesis of epidermal growth factor (EGF), a 6045 dalton mitogen produced in the mouse submaxillary gland under androgen regulation, was studied using Xenopus oocytes. Microinjection of total, unfractionated gland mRNA together with [35S]cysteine resulted in the production of a secretory polypeptide of approximately 9000 daltons, specifically immunoprecipitable with anti-EGF antibodies. A minor amount of a similarly immunoreactive 9000 dalton secretory polypeptide was produced from the sucrose gradient 9S fraction of gland mRNA. Other, more intensely labeled polypeptides, a cytoplasmic 125 000 dalton and a secretory 110 000 dalton protein were immunoprecipitated from oocytes injected with the greater than 25S mRNA fraction. The biosynthesis of both can hardly be detected in oocytes injected with unfractionated mRNA. All three polypeptides are produced under androgen regulation and share common immunoreactive properties. Northern blot analysis using a 76 nucleotide synthetic EGF cDNA probe revealed hybridization with a single 28S mRNA species. This, and the apparent interrelation between the three polypeptides, suggest that a gland-specific processing protein, encoded by a 9S mRNA, is required to produce the 9000 dalton pro-EGF from the nascent translation product of EGF mRNA.
Bravo,
Amino acid uptake in Xenopus laevis oocytes.
1976,
Pubmed
,
Xenbase
Burton,
Nerve growth factor in mouse saliva. Rapid isolation procedures for and characterization of 7 S nerve growth factor.
1978,
Pubmed
Byyny,
Radioimmunoassay of epidermal growth factor.
1972,
Pubmed
Byyny,
Epidermal growth factor: effects of androgens and adrenergic agents.
1974,
Pubmed
Colman,
Export of proteins from oocytes of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Frey,
The biosynthetic precursor of epidermal growth factor and the mechanism of its processing.
1979,
Pubmed
Gray,
Nucleotide sequence of epidermal growth factor cDNA predicts a 128,000-molecular weight protein precursor.
,
Pubmed
Gurdon,
Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.
1971,
Pubmed
,
Xenbase
Koren,
Synthetic leader peptide modulates secretion of proteins from microinjected Xenopus oocytes.
1983,
Pubmed
,
Xenbase
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lane,
The fate of genes, messengers, and proteins introduced into Xenopus oocytes.
1983,
Pubmed
,
Xenbase
Miskin,
Microinjected Xenopus oocytes synthesize active human plasminogen activator.
1981,
Pubmed
,
Xenbase
Opdenakker,
Messenger RNA for human tissue plasminogen activator.
1982,
Pubmed
,
Xenbase
Rose,
Initiation of 3T3 fibroblast cell division by epidermal growth factor.
1975,
Pubmed
Savage,
Epidermal growth factor and a new derivative. Rapid isolation procedures and biological and chemical characterization.
1972,
Pubmed
Schechter,
Further characterization of the mRNA coding for immunoglobulin light-chain.
1975,
Pubmed
Schlessinger,
Regulation of cell proliferation by epidermal growth factor.
1983,
Pubmed
Scott,
Isolation and nucleotide sequence of a cDNA encoding the precursor of mouse nerve growth factor.
1983,
Pubmed
Soreq,
Secreted proteins in the medium of microinjected Xenopus oocytes are degraded by oocyte proteases.
1981,
Pubmed
,
Xenbase
Soreq,
Biosynthesis and secretion of catalytically active acetylcholinesterase in Xenopus oocytes microinjected with mRNA from rat brain and from Torpedo electric organ.
1982,
Pubmed
,
Xenbase
