Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1982 Jan 01;15:649-54. doi: 10.1002/j.1460-2075.1982.tb01222.x.
Show Gene links
Show Anatomy links
Translation and assembly of HLA-DR antigens in Xenopus oocytes injected with mRNA from a human B-cell line.
Long EO
,
Gross N
,
Wake CT
,
Mach JP
,
Carrel S
,
Accolla R
,
Mach B
.
???displayArticle.abstract???
HLA-DR antigens are polymorphic cell surface glycoproteins, expressed primarily in B lymphocytes and macrophages, which are thought to play an important role in the immune response. Two polypeptide chains, alpha and beta, are associated at the cell surface, and a third chain associates with alpha and beta intracellularly. RNA isolated from the human B-cell line Raji was injected in Xenopus laevis oocytes. Immunoprecipitates of translation products with several monoclonal antibodies revealed the presence of HLA-DR antigens similar to those synthesized in Raji cells. One monoclonal antibody was able to bind the beta chain after dissociation of the three polypeptide chains with detergent. The presence of all three chains was confirmed by two-dimensional gel electrophoresis. The glycosylation pattern of the three chains was identical to that observed in vivo, as evidenced in studies using tunicamycin, an inhibitor of N-linked glycosylation. The presence of alpha chains assembled with beta chains in equimolar ratio was further demonstrated by amino-terminal sequencing. An RNA fraction enriched for the three mRNAs, encoding alpha, beta, and intracellular chains, was isolated. This translation-assembly system and the availability of monoclonal antibodies make it possible to assay for mRNA encoding specific molecules among the multiple human Ia-like antigens.
Accolla,
Distinct forms of both alpha and beta subunits are present in the human Ia molecular pool.
1981, Pubmed
Accolla,
Distinct forms of both alpha and beta subunits are present in the human Ia molecular pool.
1981,
Pubmed
Accolla,
Demonstration at the single-cell level of the existence of distinct clusters of epitopes in two predefined human Ia molecular subsets.
1982,
Pubmed
Allison,
Murine Ia and human DR antigens: homology of amino-terminal sequences.
1978,
Pubmed
Asselbergs,
Post-synthetic fate of the translation products of messenger RNA microinjected into Xenopus oocytes.
1979,
Pubmed
,
Xenbase
Bach,
The major histocompatibility complex--genetics and biology. (First of three parts).
1976,
Pubmed
Carrel,
Subsets of human Ia-like molecules defined by monoclonal antibodies.
1981,
Pubmed
Charron,
Characterization of HLA-D-region antigens by two-dimensional gel electrophoresis. Molecular-genotyping.
1980,
Pubmed
Charron,
Analysis of HLA-D region-associated molecules with monoclonal antibody.
1979,
Pubmed
Colman,
The influence of topology and glycosylation on the fate of heterologous secretory proteins made in Xenopus oocytes.
1981,
Pubmed
,
Xenbase
Corte,
Human Ia molecules carrying DC1 determinants differ in both alpha- and beta-subunits from Ia molecules carrying DR determinants.
1981,
Pubmed
Finn,
Multiple HLA-DR antigens: detection with monoclonal antibodies and translation in vitro.
1982,
Pubmed
,
Xenbase
Jones,
Detection of a common polypeptide chain in I--A and I--E sub-region immunoprecipitates.
1979,
Pubmed
Klein,
The major histocompatibility complex of the mouse.
1979,
Pubmed
Korman,
Cell-free synthesis and processing of the heavy and light chains of HLA-DR antigens.
1980,
Pubmed
Kratzin,
[Primary structure of class II human histocompatibility antigens. 1st communication. Amino acid sequence of the N-terminal 198 residues of the beta chain of a HLA-Dw2,2;DR2,2-alloantigen (author's transl)].
1981,
Pubmed
Lampson,
Two populations of Ia-like molecules on a human B cell line.
1980,
Pubmed
Lehrach,
RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.
1977,
Pubmed
McMillan,
Structure of murine Ia antigens. Two dimensional electrophoretic analyses and high pressure liquid chromatography tryptic peptide maps of products of the I-A and I-E subregions and of an associated invariant polypeptide.
1981,
Pubmed
Moar,
Translational capacity of living frog eggs and oocytes, as judged by messenger RNA injection.
1971,
Pubmed
Nadler,
Monoclonal antibody identifies a new Ia-like (p29,34) polymorphic system linked to the HLA-D/DR region.
1981,
Pubmed
O'Farrell,
High resolution two-dimensional electrophoresis of basic as well as acidic proteins.
1977,
Pubmed
Owen,
Biosynthesis and maturation of HLA-DR antigens in vivo.
1981,
Pubmed
Quaranta,
Purification of immunologically functional subsets of human Ia-like antigens on a monoclonal antibody (Q5/13) immunoadsorbent.
1980,
Pubmed
Rosen,
Preparation and preliminary characterization of purified ovalbumin messenger RNA from the hen oviduct.
1975,
Pubmed
Rungger,
DNAs of simian virus 40 and polyoma direct the synthesis of viral tumor antigens and capsid proteins in Xenopus oocytes.
1978,
Pubmed
,
Xenbase
Ryder,
Genetics of HLA disease association.
1981,
Pubmed
Shackelford,
Demonstration of structural polymorphism among HLA-DR light chains by two-dimensional gel electrophoresis.
1980,
Pubmed
Shackelford,
Human B-cell alloantigens DC1, MT1, and LB12 are identical to each other but distinct from the HLA-DR antigen.
1981,
Pubmed
Silver,
Structural polymorphism of human DR antigens.
1979,
Pubmed
Tosi,
Immunological dissection of human Ia molecules.
1978,
Pubmed
Walker,
Structural polymorphism of the beta chain of human HLA-DR antigens.
1980,
Pubmed
Wallace,
Protein incorporation by isolated amphibian oocytes. 3. Optimum incubation conditions.
1973,
Pubmed
,
Xenbase
Wiman,
Isolation and identification of a cDNA clone corresponding to an HLA-DR antigen beta chain.
1982,
Pubmed