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Isolation of distinct cDNA clones encoding HLA-DR beta chains by use of an expression assay.
Long EO
,
Wake CT
,
Strubin M
,
Gross N
,
Accolla RS
,
Carrel S
,
Mach B
.
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cDNA clones encoding different human Ia antigen beta chains were isolated by use of a complementation-expression assay in Xenopus oocytes. The assay was based on two previous findings. First, oocytes injected with mRNA from a human B-cell line express HLA-DR antigen. The three intracellular DR chains are assembled in oocytes and can be immunoprecipitated with anti-DR monoclonal antibodies. Second, we have isolated cDNA clones encoding DR alpha and intermediate chains. In order to identify beta-chain cDNA clones, mRNA was hybrid-selected with pools of cDNA clones, mixed with mRNA for the alpha and intermediate chains, and injected into oocytes. We isolated two distinct clones that could select DR beta-chain mRNA as demonstrated by assembly of the translation product with DR alpha chains and immunoprecipitation with DR-specific monoclonal antibodies. One clone is specific for a beta chain of the DR locus. The other clone, much weaker in its ability to select DR mRNA, encodes another Ia-like beta chain. Full-length cDNA clones corresponding to the DR and Ia-like beta chains were isolated and compared. Cross-hybridization was detectable in the coding regions but not in the 3' untranslated regions. Distinct RNAs homologous to the DR and the Ia-like beta-chain clones were present in B cells but were undetectable in three T-cell lines.
Accolla,
Distinct forms of both alpha and beta subunits are present in the human Ia molecular pool.
1981, Pubmed
Accolla,
Distinct forms of both alpha and beta subunits are present in the human Ia molecular pool.
1981,
Pubmed
Accolla,
Demonstration at the single-cell level of the existence of distinct clusters of epitopes in two predefined human Ia molecular subsets.
1982,
Pubmed
Alwine,
Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper.
1979,
Pubmed
Bach,
The major histocompatibility complex--genetics and biology. (First of three parts).
1976,
Pubmed
Charron,
Characterization of HLA-D-region antigens by two-dimensional gel electrophoresis. Molecular-genotyping.
1980,
Pubmed
Charron,
Analysis of HLA-D region-associated molecules with monoclonal antibody.
1979,
Pubmed
Chirgwin,
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
1979,
Pubmed
Clarkson,
Sequence organization of a cloned tDNA met fragment from Xenopus laevis.
1978,
Pubmed
,
Xenbase
Corte,
Human Ia molecules carrying DC1 determinants differ in both alpha- and beta-subunits from Ia molecules carrying DR determinants.
1981,
Pubmed
Goldberg,
Isolation of specific RNA's using DNA covalently linked to diazobenzyloxymethyl cellulose or paper.
1979,
Pubmed
Kafatos,
Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure.
1979,
Pubmed
Klein,
The major histocompatibility complex of the mouse.
1979,
Pubmed
Kratzin,
[Primary structure of class II human histocompatibility antigens. 1st communication. Amino acid sequence of the N-terminal 198 residues of the beta chain of a HLA-Dw2,2;DR2,2-alloantigen (author's transl)].
1981,
Pubmed
Kvist,
Membrane insertion and oligomeric assembly of HLA-DR histocompatibility antigens.
1982,
Pubmed
Larhammar,
Complete amino acid sequence of an HLA-DR antigen-like beta chain as predicted from the nucleotide sequence: similarities with immunoglobulins and HLA-A, -B, and -C antigens.
1982,
Pubmed
Long,
Translation and assembly of HLA-DR antigens in Xenopus oocytes injected with mRNA from a human B-cell line.
1982,
Pubmed
,
Xenbase
McMaster,
Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
1977,
Pubmed
Owen,
Biosynthesis and maturation of HLA-DR antigens in vivo.
1981,
Pubmed
Rigby,
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
1977,
Pubmed
Ryder,
Genetics of HLA disease association.
1981,
Pubmed
Sanger,
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
1980,
Pubmed
Shackelford,
Demonstration of structural polymorphism among HLA-DR light chains by two-dimensional gel electrophoresis.
1980,
Pubmed
Shackelford,
Human B-cell alloantigens DC1, MT1, and LB12 are identical to each other but distinct from the HLA-DR antigen.
1981,
Pubmed
Shaw,
Serologic identification of the human secondary B cell antigens. Correlations between function, genetics, and structure.
1982,
Pubmed
Silver,
Structural polymorphism of human DR antigens.
1979,
Pubmed
Tosi,
Immunological dissection of human Ia molecules.
1978,
Pubmed
Wahl,
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
1979,
Pubmed
Wake,
Isolation of cDNA clones encoding HLA-DR alpha chains.
1982,
Pubmed
,
Xenbase
Wellauer,
Isolation and sequence organization of human ribosomal DNA.
1979,
Pubmed
Wiman,
Isolation and identification of a cDNA clone corresponding to an HLA-DR antigen beta chain.
1982,
Pubmed