Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
1992 Mar 01;123:1117-25. doi: 10.1128/mcb.12.3.1117-1125.1992.
Show Gene links
Show Anatomy links
Identification of the sequences responsible for the splicing phenotype of the regulatory intron of the L1 ribosomal protein gene of Xenopus laevis.
Fragapane P
,
Caffarelli E
,
Lener M
,
Prislei S
,
Santoro B
,
Bozzoni I
.
???displayArticle.abstract???
Splicing of the regulated third intron of the L1 ribosomal protein gene of Xenopus laevis has been studied in vivo by oocyte microinjection of wild-type and mutant SP6 precursor RNAs and in vitro in the heterologous HeLa nuclear extract. We show that two different phenomena combine to produce the peculiar splicing phenotype of this intron. One, which can be defined constitutive, shows the same features in the two systems and leads to the accumulation of spliced mRNA, but in very small amounts. The low efficiency of splicing is due to the presence of a noncanonical 5' splice site which acts in conjunction with sequences present in the 3' portion of the intron. The second leads to the massive conversion of the pre-mRNA into site specific truncated molecules. This has the effect of decreasing the concentration of the pre-mRNA available for splicing. We show that this aberrant cleavage activity occurs only in the in vivo oocyte system and depends on the presence of an intact U1 RNA.
Aebi,
5' cleavage site in eukaryotic pre-mRNA splicing is determined by the overall 5' splice region, not by the conserved 5' GU.
1987, Pubmed
Aebi,
5' cleavage site in eukaryotic pre-mRNA splicing is determined by the overall 5' splice region, not by the conserved 5' GU.
1987,
Pubmed
Aebi,
Sequence requirements for splicing of higher eukaryotic nuclear pre-mRNA.
1986,
Pubmed
Al-Atia,
Translational regulation of mRNAs for ribosomal proteins during early Drosophila development.
1985,
Pubmed
Birkenmeier,
A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes.
1978,
Pubmed
,
Xenbase
Bowman,
The synthesis of ribosomal proteins S16 and L32 is not autogenously regulated during mouse myoblast differentiation.
1987,
Pubmed
Bozzoni,
Expression of two Xenopus laevis ribosomal protein genes in injected frog oocytes. A specific splicing block interferes with the L1 RNA maturation.
1984,
Pubmed
,
Xenbase
Caffarelli,
The accumulation of mature RNA for the Xenopus laevis ribosomal protein L1 is controlled at the level of splicing and turnover of the precursor RNA.
1987,
Pubmed
,
Xenbase
Chang,
Regulation by HIV Rev depends upon recognition of splice sites.
1989,
Pubmed
Dabeva,
Autogenous regulation of splicing of the transcript of a yeast ribosomal protein gene.
1986,
Pubmed
Dignam,
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
1983,
Pubmed
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Gibbs,
Ras C-terminal processing enzymes--new drug targets?
1991,
Pubmed
Gourse,
Specific binding of a prokaryotic ribosomal protein to a eukaryotic ribosomal RNA: implications for evolution and autoregulation.
1981,
Pubmed
,
Xenbase
Green,
Human beta-globin pre-mRNA synthesized in vitro is accurately spliced in Xenopus oocyte nuclei.
1983,
Pubmed
,
Xenbase
Green,
Pre-mRNA splicing.
1986,
Pubmed
Hamm,
Functional analysis of mutant Xenopus U2 snRNAs.
1989,
Pubmed
,
Xenbase
Hamm,
Monomethylated cap structures facilitate RNA export from the nucleus.
1990,
Pubmed
,
Xenbase
Kakizuka,
A set of U1 snRNA-complementary sequences involved in governing alternative RNA splicing of the kininogen genes.
1990,
Pubmed
Katz,
Control of retroviral RNA splicing through maintenance of suboptimal processing signals.
1990,
Pubmed
Krämer,
Purification of a protein required for the splicing of pre-mRNA and its separation from the lariat debranching enzyme.
1985,
Pubmed
Kuo,
Control of alternative splicing by the differential binding of U1 small nuclear ribonucleoprotein particle.
1991,
Pubmed
Loreni,
Nucleotide sequence of the L1 ribosomal protein gene of Xenopus laevis: remarkable sequence homology among introns.
1985,
Pubmed
,
Xenbase
Mariottini,
The 5' untranslated region of mRNA for ribosomal protein S19 is involved in its translational regulation during Xenopus development.
1990,
Pubmed
,
Xenbase
Mattheakis,
Retroregulation of the synthesis of ribosomal proteins L14 and L24 by feedback repressor S8 in Escherichia coli.
1989,
Pubmed
Meyuhas,
Glucocorticoids selectively inhibit translation of ribosomal protein mRNAs in P1798 lymphosarcoma cells.
1987,
Pubmed
Nagoshi,
Regulation of sex-specific RNA splicing at the Drosophila doublesex gene: cis-acting mutations in exon sequences alter sex-specific RNA splicing patterns.
1990,
Pubmed
Nomura,
Regulation of the synthesis of ribosomes and ribosomal components.
1984,
Pubmed
Peterson,
The regulated production of mu m and mu s mRNA is dependent on the relative efficiencies of mu s poly(A) site usage and the c mu 4-to-M1 splice.
1989,
Pubmed
Pierandrei-Amaldi,
Expression of the gene for ribosomal protein L1 in Xenopus embryos: alteration of gene dosage by microinjection.
1988,
Pubmed
,
Xenbase
Pierandrei-Amaldi,
Expression of ribosomal-protein genes in Xenopus laevis development.
1982,
Pubmed
,
Xenbase
Pierandrei-Amaldi,
Ribosomal protein production in normal and anucleolate Xenopus embryos: regulation at the posttranscriptional and translational levels.
1985,
Pubmed
,
Xenbase
Presutti,
The ribosomal protein L2 in S. cerevisiae controls the level of accumulation of its own mRNA.
1991,
Pubmed
Said,
Mutational analysis of the L1 binding site of 23S rRNA in Escherichia coli.
1988,
Pubmed
Shapiro,
RNA splice junctions of different classes of eukaryotes: sequence statistics and functional implications in gene expression.
1987,
Pubmed
Séraphin,
Exon mutations uncouple 5' splice site selection from U1 snRNA pairing.
1990,
Pubmed
Thomas,
Translational regulation of the L11 ribosomal protein operon of Escherichia coli: mutations that define the target site for repression by L1.
1987,
Pubmed
Weber,
In vitro splicing of mRNA precursors: 5' cleavage site can be predicted from the interaction between the 5' splice region and the 5' terminus of U1 snRNA.
1988,
Pubmed
