Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Nat Commun 2014 Jan 01;5:3516. doi: 10.1038/ncomms4516.
Show Gene links Show Anatomy links

EphrinB2 affects apical constriction in Xenopus embryos and is regulated by ADAM10 and flotillin-1.

Ji YJ , Hwang YS , Mood K , Cho HJ , Lee HS , Winterbottom E , Cousin H , Daar IO .

The Eph/ephrin signalling pathways have a critical function in cell adhesion and repulsion, and thus play key roles in various morphogenetic events during development. Here we show that a decrease in ephrinB2 protein causes neural tube closure defects during Xenopus laevis embryogenesis. Such a decrease in ephrinB2 protein levels is observed on the loss of flotillin-1 scaffold protein, a newly identified ephrinB2-binding partner. This dramatic decline in ephrinB2 protein levels on the absence of flotillin-1 expression is specific, and is partly the result of an increased susceptibility to cleavage by the metalloprotease ADAM10. These findings indicate that flotillin-1 regulates ephrinB2 protein levels through ADAM10, and is required for appropriate neural tube morphogenesis in the Xenopus embryo.

PubMed ID: 24662724
PMC ID: PMC4120273
Article link: Nat Commun
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: actl6a adam10 adam17 efnb1 efnb2 efnb3 flot1 flot2 igf2bp3 isyna1 mapk1
Morpholinos: adam10 MO1 adam13 MO4 adam17 MO1 efnb2 MO2 flot1 MO1 flot1 MO2

Article Images: [+] show captions
References [+] :
Adams, Roles of ephrinB ligands and EphB receptors in cardiovascular development: demarcation of arterial/venous domains, vascular morphogenesis, and sprouting angiogenesis. 1999, Pubmed