Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
5S rRNA gene transcription factor IIIA alters the helical configuration of DNA.
Reynolds WF
,
Gottesfeld JM
.
???displayArticle.abstract???
Relaxation of Xenopus 5S plasmid DNA (pX1o8) in the presence of transcription factor (TF) IIIA reduces the linking number of the DNA. Parallel experiments with plasmid pMB9 or cloned hepatitis B viral DNA indicate a degree of non-specific unwinding by TF; however, 60% of the effect observed for pX1o8 is due to specific interaction of TF IIIA with the 5S rRNA gene internal promoter sequence. The extent of unwinding (0.2-0.4 helical turn per TF IIIA binding site) is not consistent with the complete denaturation of the 50-base-pair TF binding site; however, it is consistent with a change in helix rotation, denaturation of 2-4 nucleotides per binding site, or DNA wrapping about a protein core. We show that proteins other than TF IIIA (bovine serum albumin and RNase) have no effect on the linking number of DNA when present during relaxation and that the unwinding activity associated with TF is heat labile. These results suggest that TF IIIA may facilitate transcription by altering the helical configuration of 5S DNA.
Arnott,
Optimised parameters for A-DNA and B-DNA.
1972, Pubmed
Arnott,
Optimised parameters for A-DNA and B-DNA.
1972,
Pubmed
Birkenmeier,
A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes.
1978,
Pubmed
,
Xenbase
Bogenhagen,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region.
1980,
Pubmed
,
Xenbase
Brown,
Cloned single repeating units of 5S DNA direct accurate transcription of 5S RNA when injected into Xenopus oocytes.
1978,
Pubmed
,
Xenbase
Champoux,
Proteins that affect DNA conformation.
1978,
Pubmed
Engelke,
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
1980,
Pubmed
,
Xenbase
Geider,
Proteins controlling the helical structure of DNA.
1981,
Pubmed
Giacherio,
A specific DNA unwinding activity associated with SV40 large T antigen.
1980,
Pubmed
Keller,
Determination of the number of superhelical turns in simian virus 40 DNA by gel electrophoresis.
1975,
Pubmed
Kolb,
Is DNA unwound by the cyclic AMP receptor protein?
1982,
Pubmed
Korn,
Transcription of Xenopus 5S ribosomal RNA genes.
1982,
Pubmed
,
Xenbase
Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed
McKay,
Structure of catabolite gene activator protein at 2.9 A resolution suggests binding to left-handed B-DNA.
1981,
Pubmed
Myers,
Does simian virus 40 T antigen unwind DNA?
1981,
Pubmed
Parker,
Selective and accurate transcription of the Xenopus laevis 5S RNA genes in isolated chromatin by purified RNA polymerase III.
1977,
Pubmed
,
Xenbase
Peck,
Sequence dependence of the helical repeat of DNA in solution.
1981,
Pubmed
Pelham,
A specific transcription factor that can bind either the 5S RNA gene or 5S RNA.
1980,
Pubmed
,
Xenbase
Sakonju,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.
1980,
Pubmed
,
Xenbase
Sanger,
The use of thin acrylamide gels for DNA sequencing.
1978,
Pubmed
Smith,
The two-dimensional restriction analysis of Drosophila DNAs: males and females.
1981,
Pubmed
Wang,
Physiochemical studies on interactions between DNA and RNA polymerase. Unwinding of the DNA helix by Escherichia coli RNA polymerase.
1977,
Pubmed
Weil,
Faithful transcription of eukaryotic genes by RNA polymerase III in systems reconstituted with purified DNA templates.
1979,
Pubmed
,
Xenbase
Wormington,
A quantitative assay for Xenopus 5S RNA gene transcription in vitro.
1981,
Pubmed
,
Xenbase
