XB-ART-26093
EMBO J
1990 Feb 01;92:573-82. doi: 10.1002/j.1460-2075.1990.tb08145.x.
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Competition between transcription complex assembly and chromatin assembly on replicating DNA.
Almouzni G
,
Méchali M
,
Wolffe AP
.
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We have used a Xenopus egg extract to show that a competition exists between the assembly of transcription complexes and nucleosomes on replicating 5S DNA. This competition results in the establishment of a transcriptionally repressed state for 5S DNA that is dependent on core histones but not on the precise positioning of the cores. The repression is selective, since satellite I DNA is not significantly repressed under these conditions. We demonstrate that the efficiency of chromatin assembly compared with transcription complex assembly is an important variable in determining gene activity.
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Species referenced: Xenopus laevis
References [+] :
Ackerman,
Molecular cloning and sequencing of OAX DNA: an abundant gene family transcribed and activated in Xenopus oocytes.
1983, Pubmed,
Xenbase
Ackerman, Molecular cloning and sequencing of OAX DNA: an abundant gene family transcribed and activated in Xenopus oocytes. 1983, Pubmed , Xenbase
Almer, Nuclease hypersensitive regions with adjacent positioned nucleosomes mark the gene boundaries of the PHO5/PHO3 locus in yeast. 1986, Pubmed
Almer, Removal of positioned nucleosomes from the yeast PHO5 promoter upon PHO5 induction releases additional upstream activating DNA elements. 1986, Pubmed
Almouzni, Assembly of spaced chromatin promoted by DNA synthesis in extracts from Xenopus eggs. 1988, Pubmed , Xenbase
Almouzni, Assembly of spaced chromatin involvement of ATP and DNA topoisomerase activity. 1988, Pubmed , Xenbase
Andersen, A model for the interaction of nucleic acids with transcription factor IIIA. 1987, Pubmed , Xenbase
Birkenmeier, A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes. 1978, Pubmed , Xenbase
Blow, Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs. 1986, Pubmed , Xenbase
Bogenhagen, Stable transcription complexes of Xenopus 5S RNA genes: a means to maintain the differentiated state. 1982, Pubmed , Xenbase
Bogenhagen, Nucleotide sequences in Xenopus 5S DNA required for transcription termination. 1981, Pubmed , Xenbase
Camerini-Otero, The organization of histones and DNA in chromatin: evidence for an arginine-rich histone kernel. 1976, Pubmed
Cortese, Transcription of tRNA genes in vivo: single-stranded compared to double-stranded templates. 1980, Pubmed , Xenbase
Cozzarelli, Purified RNA polymerase III accurately and efficiently terminates transcription of 5S RNA genes. 1983, Pubmed , Xenbase
Dilworth, Two complexes that contain histones are required for nucleosome assembly in vitro: role of nucleoplasmin and N1 in Xenopus egg extracts. 1987, Pubmed , Xenbase
Engelke, Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes. 1980, Pubmed , Xenbase
Gargiulo, Analysis of the chromatin assembled in germinal vesicles of Xenopus oocytes. 1983, Pubmed , Xenbase
Germond, Folding of the DNA double helix in chromatin-like structures from simian virus 40. 1975, Pubmed
Gottesfeld, Assembly of transcriptionally active 5S RNA gene chromatin in vitro. 1982, Pubmed , Xenbase
Gralla, Rapid "footprinting" on supercoiled DNA. 1985, Pubmed
Han, Nucleosome loss activates yeast downstream promoters in vivo. 1988, Pubmed
Han, Depletion of histone H4 and nucleosomes activates the PHO5 gene in Saccharomyces cerevisiae. 1988, Pubmed
Hanas, Binding of Xenopus transcription factor A to 5S RNA and to single stranded DNA. 1984, Pubmed , Xenbase
Hanas, Xenopus transcription factor A promotes DNA reassociation. 1985, Pubmed , Xenbase
Hand, Eucaryotic DNA: organization of the genome for replication. 1978, Pubmed
Hayes, A protein-protein interaction is essential for stable complex formation on a 5 S RNA gene. 1989, Pubmed , Xenbase
Huberman, New views of the biochemistry of eucaryotic DNA replication revealed by aphidicolin, an unusual inhibitor of DNA polymerase alpha. 1981, Pubmed
Hutchison, Periodic DNA synthesis in cell-free extracts of Xenopus eggs. 1987, Pubmed , Xenbase
Jahn, Transcription complexes for various class III genes differ in parameters of formation and stability towards salt. 1987, Pubmed
Knezetic, The presence of nucleosomes on a DNA template prevents initiation by RNA polymerase II in vitro. 1986, Pubmed , Xenbase
Ladiges, The canine major histocompatibility complex. Supertypic specificities defined by the primed lymphocyte test (PLT). 1984, Pubmed
Lam, Tandemly repeated DNA sequences from Xenopus laevis. I. Studies on sequence organization and variation in satellite 1 DNA (741 base-pair repeat). 1983, Pubmed , Xenbase
Lassar, Stable transcription complex on a class III gene in a minichromosome. 1985, Pubmed , Xenbase
Méchali, DNA synthesis in a cell-free system from Xenopus eggs: priming and elongation on single-stranded DNA in vitro. 1982, Pubmed , Xenbase
Morse, Nucleosomes inhibit both transcriptional initiation and elongation by RNA polymerase III in vitro. 1989, Pubmed , Xenbase
Noll, Action of micrococcal nuclease on chromatin and the location of histone H1. 1977, Pubmed
Palter, Evidence for the formation of nucleosome-like histone complexes on single-stranded DNA. 1979, Pubmed
Peterson, Characterization of two xenopus somatic 5S DNAs and one minor oocyte-specific 5S DNA. 1980, Pubmed , Xenbase
Pieler, Analysis of the RNA structural elements involved in the binding of the transcription factor III A from Xenopus laevis. 1986, Pubmed , Xenbase
Pieler, The 5S gene internal control region is composed of three distinct sequence elements, organized as two functional domains with variable spacing. 1987, Pubmed , Xenbase
Rhodes, Structural analysis of a triple complex between the histone octamer, a Xenopus gene for 5S RNA and transcription factor IIIA. 1985, Pubmed , Xenbase
Roeder, Multiple forms of deoxyribonucleic acid-dependent ribonucleic acid polymerase in Xenopus laevis. Isolation and partial characterization. 1974, Pubmed , Xenbase
Sakonju, Contact points between a positive transcription factor and the Xenopus 5S RNA gene. 1982, Pubmed , Xenbase
Sakonju, A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region. 1980, Pubmed , Xenbase
Schlissel, The transcriptional regulation of Xenopus 5s RNA genes in chromatin: the roles of active stable transcription complexes and histone H1. 1984, Pubmed , Xenbase
Segall, Multiple factors are required for the accurate transcription of purified genes by RNA polymerase III. 1980, Pubmed
Senshu, Preferential association of newly synthesized H3 and H4 histones with newly replicated DNA. 1978, Pubmed
Setzer, Formation and stability of the 5 S RNA transcription complex. 1985, Pubmed , Xenbase
Shastry, Multiple factors involved in the transcription of class III genes in Xenopus laevis. 1982, Pubmed , Xenbase
Shimamura, Characterization of the repressed 5S DNA minichromosomes assembled in vitro with a high-speed supernatant of Xenopus laevis oocytes. 1988, Pubmed , Xenbase
Simon, A new procedure for purifying histone pairs H2A + H2B and H3 + H4 from chromatin using hydroxylapatite. 1979, Pubmed
Smith, Domains of the positive transcription factor specific for the Xenopus 5S RNA gene. 1984, Pubmed , Xenbase
Spadafora, Rearrangement of chromatin structure induced by increasing ionic strength and temperature. 1979, Pubmed
Stillman, Chromatin assembly during SV40 DNA replication in vitro. 1986, Pubmed
Stillman, Effects of temperature and single-stranded DNA on the interaction of an RNA polymerase III transcription factor with a tRNA gene. 1985, Pubmed
Wakefield, The activation of RNA synthesis by somatic nuclei injected into amphibian oocytes. 1983, Pubmed , Xenbase
Weisbrod, Active chromatin of oocytes injected with somatic cell nuclei or cloned DNA. 1982, Pubmed , Xenbase
Wold, Identification of cellular proteins required for simian virus 40 DNA replication. 1989, Pubmed
Wolffe, DNA replication in vitro erases a Xenopus 5S RNA gene transcription complex. 1986, Pubmed , Xenbase
Wolffe, A bacteriophage RNA polymerase transcribes through a Xenopus 5S RNA gene transcription complex without disrupting it. 1986, Pubmed , Xenbase
Wolffe, Differential 5S RNA gene expression in vitro. 1987, Pubmed , Xenbase
Wolffe, Transient paralysis by heat shock of hormonal regulation of gene expression. 1984, Pubmed , Xenbase
Wolffe, Transcription fraction TFIIIC can regulate differential Xenopus 5S RNA gene transcription in vitro. 1988, Pubmed , Xenbase
Wolffe, Developmental regulation of two 5S ribosomal RNA genes. 1988, Pubmed , Xenbase
Wolffe, Transcriptional activation of Xenopus class III genes in chromatin isolated from sperm and somatic nuclei. 1989, Pubmed , Xenbase
Wolffe, Dominant and specific repression of Xenopus oocyte 5S RNA genes and satellite I DNA by histone H1. 1989, Pubmed , Xenbase
Worcel, Assembly of newly replicated chromatin. 1978, Pubmed
Workman, Transcriptional regulation by the immediate early protein of pseudorabies virus during in vitro nucleosome assembly. 1988, Pubmed , Xenbase
Workman, Binding of transcription factor TFIID to the major late promoter during in vitro nucleosome assembly potentiates subsequent initiation by RNA polymerase II. 1987, Pubmed , Xenbase
Wormington, Onset of 5 S RNA gene regulation during Xenopus embryogenesis. 1983, Pubmed , Xenbase
Wormington, A quantitative assay for Xenopus 5S RNA gene transcription in vitro. 1981, Pubmed , Xenbase