Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
The primary structure of the larval beta 1-globin gene of Xenopus laevis and its flanking regions.
Meyerhof W
,
Klinger-Mitropoulos S
,
Stalder J
,
Weber R
,
Knöchel W
.
???displayArticle.abstract???
We present the complete nucleotide sequence of the larval beta I-globin gene of Xenopus laevis including 240 nucleotides of the 5' flanking region and 594 nucleotides beyond the polyadenylation site. The site of transcription initiation was mapped by S1 nuclease, and the site of polyadenylation was determined by comparison with corresponding cDNA clones. The larval Xenopus beta I-gene shows the same internal structure as the beta-globin genes of higher vertebrates, viz. 3 exons interrupted by 2 intervening sequences. The first intervening sequence, which is of exceptional length, spans over 564 nucleotides and interrupts the coding sequence at amino acid 30, whereas the second one comprises 968 nucleotides and is located between the amino acids 104 and 105. The second intervening sequence contains a long inverted repeat of almost perfect homology. The 5' flanking region contains a TATA- and a CAAT-box at positions -33 and -58, respectively. An additional TATA-box is located at -197 and two more CAAT-boxes occur at positions -105 and -237.
Ackerman,
Molecular cloning and sequencing of OAX DNA: an abundant gene family transcribed and activated in Xenopus oocytes.
1983, Pubmed,
Xenbase
Ackerman,
Molecular cloning and sequencing of OAX DNA: an abundant gene family transcribed and activated in Xenopus oocytes.
1983,
Pubmed
,
Xenbase
Alan,
Epsilon globin gene transcripts originating upstream of the mRNA cap site in K562 cells and normal human embryos.
1982,
Pubmed
Allan,
Multiple origins of transcription in the 4.5 Kb upstream of the epsilon-globin gene.
1983,
Pubmed
Andres,
Comparative analysis of the cDNA sequences derived from the larval and the adult alpha 1-globin mRNAs of Xenopus laevis.
1984,
Pubmed
,
Xenbase
Aviv,
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
1972,
Pubmed
Banville,
The nucleotide sequence of the mRNA encoding a tadpole beta-globin polypeptide of Xenopus laevis.
1983,
Pubmed
,
Xenbase
Baralle,
AUG is the only recognisable signal sequence in the 5' non-coding regions of eukaryotic mRNA.
1978,
Pubmed
Benoist,
The ovalbumin gene-sequence of putative control regions.
1980,
Pubmed
Birnboim,
A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
1979,
Pubmed
Breathnach,
Ovalbumin gene: evidence for a leader sequence in mRNA and DNA sequences at the exon-intron boundaries.
1978,
Pubmed
Breathnach,
Organization and expression of eucaryotic split genes coding for proteins.
1981,
Pubmed
Crouse,
An integrated and simplified approach to cloning into plasmids and single-stranded phages.
1983,
Pubmed
Degrave,
Nucleotide sequence of the chromosomal gene for human fibroblast (beta 1) interferon and of the flanking regions.
1981,
Pubmed
Dierks,
Three regions upstream from the cap site are required for efficient and accurate transcription of the rabbit beta-globin gene in mouse 3T6 cells.
1983,
Pubmed
Dodgson,
The nucleotide sequence of the embryonic chicken beta-type globin genes.
1983,
Pubmed
Efstratiadis,
The structure and evolution of the human beta-globin gene family.
1980,
Pubmed
Engel,
Unusual structure of the chicken embryonic alpha-globin gene, pi'.
1983,
Pubmed
Fermi,
Three-dimensional fourier synthesis of human deoxyhaemoglobin at 2-5 A resolution: refinement of the atomic model.
1975,
Pubmed
Fiers,
Complete nucleotide sequence of SV40 DNA.
1978,
Pubmed
Grindlay,
Alternative sites of transcription initiation upstream of the canonical cap site in human gamma-globin and beta-globin genes.
1984,
Pubmed
Grosschedl,
Spacer DNA sequences upstream of the T-A-T-A-A-A-T-A sequence are essential for promotion of H2A histone gene transcription in vivo.
1980,
Pubmed
,
Xenbase
Hagenbüchle,
Conservation of the primary structure at the 3' end of 18S rRNA from eucaryotic cells.
1978,
Pubmed
Hardison,
The nucleotide sequence of the rabbit embryonic globin gene beta 4.
1983,
Pubmed
Hentschel,
Analysis of Xenopus laevis globins during development and erythroid cell maturation and the construction of recombinant plasmids containing sequences derived from adult globin mRNA.
1979,
Pubmed
,
Xenbase
Hosbach,
The Xenopus laevis globin gene family: chromosomal arrangement and gene structure.
1983,
Pubmed
,
Xenbase
Jeffreys,
Linkage of adult alpha- and beta-globin genes in X. laevis and gene duplication by tetraploidization.
1980,
Pubmed
,
Xenbase
Jeffreys,
Isolation and sequence analysis of a hybrid delta-globin pseudogene from the brown lemur.
1982,
Pubmed
Kay,
Complete nucleotide sequence of a cloned cDNA derived from the major adult alpha-globin mRNA of X. laevis.
1983,
Pubmed
,
Xenbase
Knöchel,
Molecular cloning and sequencing of mRNAs coding for minor adult globin polypeptides of Xenopus laevis.
1983,
Pubmed
,
Xenbase
Lacy,
The nucleotide sequence of a rabbit beta-globin pseudogene.
1980,
Pubmed
Lawn,
DNA sequence of a major human leukocyte interferon gene.
1981,
Pubmed
Mattaj,
Xenopus laevis U2 snRNA genes: tandemly repeated transcription units sharing 5' and 3' flanking homology with other RNA polymerase II transcribed genes.
1983,
Pubmed
,
Xenbase
Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed
May,
Isolation and characterisation of the Xenopus laevis albumin genes: loss of 74K albumin gene sequences by library amplification.
1982,
Pubmed
,
Xenbase
McKnight,
Transcriptional control signals of a eukaryotic protein-coding gene.
1982,
Pubmed
Messing,
A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
1982,
Pubmed
Nagata,
The structure of one of the eight or more distinct chromosomal genes for human interferon-alpha.
1980,
Pubmed
Partington,
Isolation of a Xenopus laevis alpha-globin gene.
1981,
Pubmed
,
Xenbase
Patient,
The organization of the tadpole and adult alpha globin genes of Xenopus laevis.
1982,
Pubmed
,
Xenbase
Patient,
The complete nucleotide sequence of the major adult beta globin gene of Xenopus laevis.
1983,
Pubmed
,
Xenbase
Poncz,
"Nonrandom" DNA sequence analysis in bacteriophage M13 by the dideoxy chain-termination method.
1982,
Pubmed
Proudfoot,
The structure of the human zeta-globin gene and a closely linked, nearly identical pseudogene.
1982,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Sanger,
The use of thin acrylamide gels for DNA sequencing.
1978,
Pubmed
Schon,
Structure and evolution of goat gamma-, beta C- and beta A-globin genes: three developmentally regulated genes contain inserted elements.
1981,
Pubmed
Spritz,
Complete nucleotide sequence of the human delta-globin gene.
1980,
Pubmed
Taya,
Cloning and structure of the human immune interferon-gamma chromosomal gene.
1982,
Pubmed
,
Xenbase
Weaver,
Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates.
1979,
Pubmed
Widmer,
Comparative analysis of cloned larval and adult globin cDNA sequences of Xenopus laevis.
1981,
Pubmed
,
Xenbase
Williams,
The nucleotide sequence of the major beta-globin mRNA from Xenopus laevis.
1980,
Pubmed
,
Xenbase
van Ooyen,
Comparison of total sequence of a cloned rabbit beta-globin gene and its flanking regions with a homologous mouse sequence.
1979,
Pubmed