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???displayArticle.abstract??? CPEB is an RNA binding protein that interacts with the maturation-type cytoplasmic polyadenylation element (CPE) (consensus UUUUUAU) to promote polyadenylation and translational activation of maternal mRNAs in Xenopus laevis. CPEB, which is conserved from mammals to invertebrates, is composed of three regions: an amino-terminal portion with no obvious functional motif, two RNA recognition motifs (RRMs), and a cysteine-histidine region that is reminiscent of a zinc finger. In this study, we investigated the physical properties of CPEB required for RNA binding. CPEB can interact with RNA as a monomer, and phosphorylation, which modifies the protein during oocyte maturation, has little effect on RNA binding. Deletion mutations of CPEB have been overexpressed in Escherichia coli and used in a series of RNA gel shift experiments. Although a full-length and a truncated CPEB that lacks 139 amino-terminal amino acids bind CPE-containing RNA avidly, proteins that have had either RRM deleted bind RNA much less efficiently. CPEB that has had the cysteine-histidine region deleted has no detectable capacity to bind RNA. Single alanine substitutions of specific cysteine or histidine residues within this region also abolish RNA binding, pointing to the importance of this highly conserved domain of the protein. Chelation of metal ions by 1,10-phenanthroline inhibits the ability of CPEB to bind RNA; however, RNA binding is restored if the reaction is supplemented with zinc. CPEB also binds other metals such as cobalt and cadmium, but these destroy RNA binding. These data indicate that the RRMs and a zinc finger region of CPEB are essential for RNA binding.
Auld,
Use of chelating agents to inhibit enzymes.
1988, Pubmed
Auld,
Use of chelating agents to inhibit enzymes.
1988,
Pubmed Berg,
The galvanization of biology: a growing appreciation for the roles of zinc.
1996,
Pubmed Bilger,
Nuclear polyadenylation factors recognize cytoplasmic polyadenylation elements.
1994,
Pubmed
,
Xenbase Bouvet,
A role for transcription and FRGY2 in masking maternal mRNA within Xenopus oocytes.
1994,
Pubmed
,
Xenbase Burd,
Conserved structures and diversity of functions of RNA-binding proteins.
1994,
Pubmed Burd,
The multiple RNA-binding domains of the mRNA poly(A)-binding protein have different RNA-binding activities.
1991,
Pubmed
,
Xenbase Carey,
An amino-terminal fragment of GAL4 binds DNA as a dimer.
1989,
Pubmed Cavaloc,
Characterization and cloning of the human splicing factor 9G8: a novel 35 kDa factor of the serine/arginine protein family.
1994,
Pubmed Chung,
Purification and properties of HuD, a neuronal RNA-binding protein.
1996,
Pubmed Dalby,
Discrete sequence elements control posterior pole accumulation and translational repression of maternal cyclin B RNA in Drosophila.
1993,
Pubmed de Moor,
The Mos pathway regulates cytoplasmic polyadenylation in Xenopus oocytes.
1997,
Pubmed
,
Xenbase Gebauer,
Translational control by cytoplasmic polyadenylation of c-mos mRNA is necessary for oocyte maturation in the mouse.
1994,
Pubmed Gebauer,
Mouse cytoplasmic polyadenylylation element binding protein: an evolutionarily conserved protein that interacts with the cytoplasmic polyadenylylation elements of c-mos mRNA.
1996,
Pubmed
,
Xenbase Hake,
CPEB is a specificity factor that mediates cytoplasmic polyadenylation during Xenopus oocyte maturation.
1994,
Pubmed
,
Xenbase Hake,
Translational regulation of maternal mRNA.
1997,
Pubmed
,
Xenbase Kim-Ha,
Translational regulation of oskar mRNA by bruno, an ovarian RNA-binding protein, is essential.
1995,
Pubmed Koushika,
ELAV, a Drosophila neuron-specific protein, mediates the generation of an alternatively spliced neural protein isoform.
1996,
Pubmed Kuge,
Cytoplasmic 3' poly(A) addition induces 5' cap ribose methylation: implications for translational control of maternal mRNA.
1995,
Pubmed
,
Xenbase Labbé,
Purification of mouse MEP-1, a nuclear protein which binds to the metal regulatory elements of genes encoding metallothionein.
1993,
Pubmed Lane,
Use of gel retardation to analyze protein-nucleic acid interactions.
1992,
Pubmed Lantz,
The Drosophila orb gene is predicted to encode sex-specific germline RNA-binding proteins and has localized transcripts in ovaries and early embryos.
1992,
Pubmed Lee,
Zinc stimulates Mg2+-dependent 3'-processing activity of human immunodeficiency virus type 1 integrase in vitro.
1996,
Pubmed Levine,
Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs.
1993,
Pubmed Lutz,
Direct interaction of the U1 snRNP-A protein with the upstream efficiency element of the SV40 late polyadenylation signal.
1994,
Pubmed McGrew,
Poly(A) elongation during Xenopus oocyte maturation is required for translational recruitment and is mediated by a short sequence element.
1989,
Pubmed
,
Xenbase Navaratnam,
The p27 catalytic subunit of the apolipoprotein B mRNA editing enzyme is a cytidine deaminase.
1993,
Pubmed
,
Xenbase Paris,
Maturation-specific polyadenylation: in vitro activation by p34cdc2 and phosphorylation of a 58-kD CPE-binding protein.
1991,
Pubmed
,
Xenbase Predki,
Effect of replacement of "zinc finger" zinc on estrogen receptor DNA interactions.
1992,
Pubmed Richter,
Reversible inhibition of translation by Xenopus oocyte-specific proteins.
,
Pubmed
,
Xenbase Robbie,
Temporal regulation of the Xenopus FGF receptor in development: a translation inhibitory element in the 3' untranslated region.
1995,
Pubmed
,
Xenbase Sallés,
Coordinate initiation of Drosophila development by regulated polyadenylation of maternal messenger RNAs.
1994,
Pubmed Sánchez-García,
The LIM domain: a new structural motif found in zinc-finger-like proteins.
1994,
Pubmed Sheets,
Polyadenylation of c-mos mRNA as a control point in Xenopus meiotic maturation.
1995,
Pubmed
,
Xenbase Standart,
Maternal mRNA from clam oocytes can be specifically unmasked in vitro by antisense RNA complementary to the 3'-untranslated region.
1990,
Pubmed Stebbins-Boaz,
CPEB controls the cytoplasmic polyadenylation of cyclin, Cdk2 and c-mos mRNAs and is necessary for oocyte maturation in Xenopus.
1996,
Pubmed
,
Xenbase Stebbins-Boaz,
Multiple sequence elements and a maternal mRNA product control cdk2 RNA polyadenylation and translation during early Xenopus development.
1994,
Pubmed
,
Xenbase St Johnston,
The origin of pattern and polarity in the Drosophila embryo.
1992,
Pubmed Strickland,
Antisense RNA directed against the 3' noncoding region prevents dormant mRNA activation in mouse oocytes.
1988,
Pubmed Wilson,
2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans.
1994,
Pubmed
