Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1996 Nov 12;9323:12964-8. doi: 10.1073/pnas.93.23.12964.
Show Gene links
Show Anatomy links
Rapid chemical kinetic techniques for investigations of neurotransmitter receptors expressed in Xenopus oocytes.
Niu L
,
Vazquez RW
,
Nagel G
,
Friedrich T
,
Bamberg E
,
Oswald RE
,
Hess GP
.
???displayArticle.abstract???
Xenopus laevis oocytes have been used extensively during the past decade to express and study neurotransmitter receptors of various origins and subunit composition and also to express and study receptors altered by site-specific mutations. Interpretations of the effects of structural differences on receptor mechanisms were, however, hampered by a lack of rapid chemical reaction techniques suitable for use with oocytes. Here we describe flow and photolysis techniques, with 2-ms and 100-microseconds time resolution, respectively, for studying neurotransmitter receptors in giant (approximately 20-microns diameter) patches of oocyte membranes, using muscle and neuronal acetylcholine receptors as examples. With these techniques, we find that the muscle receptor in BC3H1 cells and the same receptor expressed in oocytes have comparable kinetic properties. This finding is in contrast to previous studies and raises questions regarding the interpretations of the many studies of receptors expressed in oocytes in which an insufficient time resolution was available. The results obtained indicate that the rapid reaction techniques described here, in conjunction with the oocyte expression system, will be useful in answering many outstanding questions regarding the structure and function of diverse neurotransmitter receptors.
Barnard,
Translation of exogenous messenger RNA coding for nicotinic acetylcholine receptors produces functional receptors in Xenopus oocytes.
1982, Pubmed,
Xenbase
Barnard,
Translation of exogenous messenger RNA coding for nicotinic acetylcholine receptors produces functional receptors in Xenopus oocytes.
1982,
Pubmed
,
Xenbase
Boulter,
Isolation of a clone coding for the alpha-subunit of a mouse acetylcholine receptor.
1985,
Pubmed
Costa,
Improved technique for studying ion channels expressed in Xenopus oocytes, including fast superfusion.
1994,
Pubmed
,
Xenbase
Dascal,
Role of calcium mobilization in mediation of acetylcholine-evoked chloride currents in Xenopus laevis oocytes.
1985,
Pubmed
,
Xenbase
Dilger,
The temperature dependence of some kinetic and conductance properties of acetylcholine receptor channels.
1991,
Pubmed
Dilger,
Desensitization of acetylcholine receptors in BC3H-1 cells.
1992,
Pubmed
Fong,
Correlation between acetylcholine receptor function and structural properties of membranes.
1986,
Pubmed
Geetha,
On the mechanism of the gamma-aminobutyric acid receptor in the mammalian (mouse) cerebral cortex. Chemical kinetic investigations with a 10-ms time resolution adapted to measurements of neuronal receptor function in single cells.
1992,
Pubmed
Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed
Hess,
Determination of the chemical mechanism of neurotransmitter receptor-mediated reactions by rapid chemical kinetic methods.
1995,
Pubmed
Hilgemann,
Giant excised cardiac sarcolemmal membrane patches: sodium and sodium-calcium exchange currents.
1989,
Pubmed
KALTENBACH,
Nigrosin as a dye for differentiating live and dead ascites cells.
1958,
Pubmed
KATZ,
A study of the desensitization produced by acetylcholine at the motor end-plate.
1957,
Pubmed
Krishtal,
A receptor for protons in the nerve cell membrane.
1980,
Pubmed
LaPolla,
Isolation and characterization of a cDNA clone for the complete protein coding region of the delta subunit of the mouse acetylcholine receptor.
1984,
Pubmed
Langosch,
Decreased agonist affinity and chloride conductance of mutant glycine receptors associated with human hereditary hyperekplexia.
1994,
Pubmed
,
Xenbase
Leonard,
Evidence that the M2 membrane-spanning region lines the ion channel pore of the nicotinic receptor.
1988,
Pubmed
,
Xenbase
Matsubara,
How fast does an acetylcholine receptor channel open? Laser-pulse photolysis of an inactive precursor of carbamoylcholine in the microsecond time region with BC3H1 cells.
1992,
Pubmed
Merlie,
cDNA clone for the alpha subunit of the acetylcholine receptor from the mouse muscle cell line BC3H-1.
1983,
Pubmed
Methfessel,
Patch clamp measurements on Xenopus laevis oocytes: currents through endogenous channels and implanted acetylcholine receptor and sodium channels.
1986,
Pubmed
,
Xenbase
Milburn,
Synthesis, photochemistry, and biological activity of a caged photolabile acetylcholine receptor ligand.
1989,
Pubmed
Neher,
Single-channel currents recorded from membrane of denervated frog muscle fibres.
1976,
Pubmed
Niu,
An acetylcholine receptor regulatory site in BC3H1 cells: characterized by laser-pulse photolysis in the microsecond-to-millisecond time region.
1993,
Pubmed
Niu,
Cocaine: mechanism of inhibition of a muscle acetylcholine receptor studied by a laser-pulse photolysis technique.
1995,
Pubmed
Puchacz,
Functional expression of nicotinic acetylcholine receptors containing rat alpha 7 subunits in human SH-SY5Y neuroblastoma cells.
1994,
Pubmed
,
Xenbase
Samson,
Neurobiology of alcohol abuse.
1992,
Pubmed
Schubert,
Characterization of a unique muscle cell line.
1974,
Pubmed
Stafford,
The beta subunit of neuronal nicotinic acetylcholine receptors is a determinant of the affinity for substance P inhibition.
1994,
Pubmed
,
Xenbase
Séguéla,
Molecular cloning, functional properties, and distribution of rat brain alpha 7: a nicotinic cation channel highly permeable to calcium.
1993,
Pubmed
,
Xenbase
Udgaonkar,
Chemical kinetic measurements of a mammalian acetylcholine receptor by a fast-reaction technique.
1987,
Pubmed
Vernino,
Calcium modulation and high calcium permeability of neuronal nicotinic acetylcholine receptors.
1992,
Pubmed
,
Xenbase
Villarroel,
Dimensions of the narrow portion of a recombinant NMDA receptor channel.
1995,
Pubmed
,
Xenbase
Yu,
Mouse muscle nicotinic acetylcholine receptor gamma subunit: cDNA sequence and gene expression.
1986,
Pubmed
