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A 12-base-pair sequence is an essential element of the ribosomal gene terminator in Xenopus laevis.
Labhart P
,
Reeder RH
.
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rRNA transcription in Xenopus laevis terminates near a 7-base-pair (bp) conserved sequence (T3 box) located 200 bp upstream of the site of transcription initiation for the adjacent gene promoter. We present evidence here that a 12-bp element containing the T3 box is an essential part of the terminator. Using an oocyte injection assay, we found that the 12-bp element (but not the T3 box alone) severely reduced the amount of RNA detectable at sites downstream from itself and that the T3 box within the 12-bp element was required to specify the formation of correct 3' ends. This requirement for the 12-bp element was also seen in pulse-label experiments by using a homogenate of oocyte nuclei, but the present data did not allow us to determine the exact mechanism by which the 12-bp element acts. Removal of the T3 region from its normal location allowed a significant amount of readthrough transcripts to accumulate, indicating that additional sequences may be required for complete terminator function.
Boseley,
Sequence organization of the spacer DNA in a ribosomal gene unit of Xenopus laevis.
1979, Pubmed,
Xenbase
Boseley,
Sequence organization of the spacer DNA in a ribosomal gene unit of Xenopus laevis.
1979,
Pubmed
,
Xenbase
De Winter,
The ribosomal spacer in Xenopus laevis is transcribed as part of the primary ribosomal RNA.
1986,
Pubmed
,
Xenbase
Delli Bovi,
Isolation of a rearranged human transforming gene following transfection of Kaposi sarcoma DNA.
1987,
Pubmed
Furlong,
The external transcribed spacer and preceding region of Xenopus borealis rDNA: comparison with the corresponding region of Xenopus laevis rDNA.
1983,
Pubmed
,
Xenbase
Grummt,
Transcription of mouse rDNA terminates downstream of the 3' end of 28S RNA and involves interaction of factors with repeated sequences in the 3' spacer.
1985,
Pubmed
Grummt,
A repeated 18 bp sequence motif in the mouse rDNA spacer mediates binding of a nuclear factor and transcription termination.
1986,
Pubmed
Grummt,
A transcription terminator located upstream of the mouse rDNA initiation site affects rRNA synthesis.
1986,
Pubmed
Henderson,
A transcriptional terminator is a novel element of the promoter of the mouse ribosomal RNA gene.
1986,
Pubmed
Hipskind,
Initiation of ribosomal RNA chains in homogenates of oocyte nuclei.
1980,
Pubmed
,
Xenbase
Labhart,
Xenopus ribosomal gene enhancers function when inserted inside the gene they enhance.
1985,
Pubmed
,
Xenbase
Labhart,
Characterization of three sites of RNA 3' end formation in the Xenopus ribosomal gene spacer.
1986,
Pubmed
,
Xenbase
Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed
McStay,
A termination site for Xenopus RNA polymerase I also acts as an element of an adjacent promoter.
1986,
Pubmed
,
Xenbase
Moss,
More ribosomal spacer sequences from Xenopus laevis.
1980,
Pubmed
,
Xenbase
Moss,
The promotion of ribosomal transcription in eukaryotes.
1985,
Pubmed
Moss,
A transcriptional function for the repetitive ribosomal spacer in Xenopus laevis.
,
Pubmed
,
Xenbase
Norris,
Efficient site-directed mutagenesis by simultaneous use of two primers.
1983,
Pubmed
Sollner-Webb,
Nested control regions promote Xenopus ribosomal RNA synthesis by RNA polymerase I.
1983,
Pubmed
,
Xenbase