Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1988 Dec 01;8524:9669-73.
Show Gene links
Show Anatomy links
Isolation and characterization of a Xenopus laevis C protein cDNA: structure and expression of a heterogeneous nuclear ribonucleoprotein core protein.
Preugschat F
,
Wold B
.
???displayArticle.abstract???
The C proteins are major components of heterogeneous nuclear ribonucleoprotein complexes in nuclei of vertebrate cells. To begin to describe their structure, expression, and function we isolated and determined the DNA sequence of Xenopus laevis C protein cDNA clones. The protein predicted from the DNA sequence has a molecular mass of 30,916 kDa and is very similar to its human counterpart. Although mammalian genomes contain many copies of C protein sequence, the Xenopus genome contains few copies. When C protein RNA was synthesized in vitro and microinjected into stage-VI Xenopus oocytes, newly synthesized C proteins were efficiently localized in the nucleus. In vitro rabbit reticulocyte lysate and in vivo Xenopus oocyte translation systems both produce from a single mRNA two discrete polypeptide species that accumulate in a ratio similar to that of mammalian C1 and C2 proteins in vivo.
Adam,
mRNA polyadenylate-binding protein: gene isolation and sequencing and identification of a ribonucleoprotein consensus sequence.
1986, Pubmed
Adam,
mRNA polyadenylate-binding protein: gene isolation and sequencing and identification of a ribonucleoprotein consensus sequence.
1986,
Pubmed
Adeniyi-Jones,
Transcription, processing and nuclear transport of a B1 Alu RNA species complementary to an intron of the murine alpha-fetoprotein gene.
,
Pubmed
,
Xenbase
Beyer,
Identification and characterization of the packaging proteins of core 40S hnRNP particles.
1977,
Pubmed
Beyer,
Correlation of hnRNP structure and nascent transcript cleavage.
1981,
Pubmed
Buvoli,
cDNA cloning of human hnRNP protein A1 reveals the existence of multiple mRNA isoforms.
1988,
Pubmed
Chirgwin,
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
1979,
Pubmed
Choi,
Isolation of the heterogeneous nuclear RNA-ribonucleoprotein complex (hnRNP): a unique supramolecular assembly.
1984,
Pubmed
Choi,
Heterogeneous nuclear ribonucleoproteins: role in RNA splicing.
1986,
Pubmed
Chung,
Set of novel, conserved proteins fold pre-messenger RNA into ribonucleosomes.
1986,
Pubmed
Cobianchi,
Structure of rodent helix-destabilizing protein revealed by cDNA cloning.
1986,
Pubmed
Conway,
A sequence downstream of A-A-U-A-A-A is required for formation of simian virus 40 late mRNA 3' termini in frog oocytes.
1985,
Pubmed
,
Xenbase
De Robertis,
Intranuclear location of the tRNA splicing enzymes.
1981,
Pubmed
,
Xenbase
Dingwall,
Nucleoplasmin cDNA sequence reveals polyglutamic acid tracts and a cluster of sequences homologous to putative nuclear localization signals.
1987,
Pubmed
,
Xenbase
Dreyfuss,
Characterization of heterogeneous nuclear RNA-protein complexes in vivo with monoclonal antibodies.
1984,
Pubmed
Dreyfuss,
Structure and function of nuclear and cytoplasmic ribonucleoprotein particles.
1986,
Pubmed
Earnshaw,
Assembly of nucleosomes: the reaction involving X. laevis nucleoplasmin.
1980,
Pubmed
,
Xenbase
Fitzgerald,
The sequence 5'-AAUAAA-3'forms parts of the recognition site for polyadenylation of late SV40 mRNAs.
1981,
Pubmed
Foe,
Comparative organization of active transcription units in Oncopeltus fasciatus.
1976,
Pubmed
Galli,
Biochemical complementation with RNA in the Xenopus oocyte: a small RNA is required for the generation of 3' histone mRNA termini.
1983,
Pubmed
,
Xenbase
Georgiev,
Processing and nucleo-cytoplasmic transport of histone gene transcripts.
1984,
Pubmed
,
Xenbase
Grange,
Human mRNA polyadenylate binding protein: evolutionary conservation of a nucleic acid binding motif.
1987,
Pubmed
,
Xenbase
Green,
Human beta-globin pre-mRNA synthesized in vitro is accurately spliced in Xenopus oocyte nuclei.
1983,
Pubmed
,
Xenbase
Habets,
Analysis of a cDNA clone expressing a human autoimmune antigen: full-length sequence of the U2 small nuclear RNA-associated B" antigen.
1987,
Pubmed
Hathaway,
Casein kinases--multipotential protein kinases.
1982,
Pubmed
Hattori,
Dideoxy sequencing method using denatured plasmid templates.
1986,
Pubmed
Haynes,
pen repeat sequences are GGN clusters and encode a glycine-rich domain in a Drosophila cDNA homologous to the rat helix destabilizing protein.
1987,
Pubmed
Holcomb,
Phosphorylation of the C-proteins of HeLa cell hnRNP particles. Involvement of a casein kinase II-type enzyme.
1984,
Pubmed
Jong,
Saccharomyces cerevisiae SSB1 protein and its relationship to nucleolar RNA-binding proteins.
1987,
Pubmed
Kleinschmidt,
Co-existence of two different types of soluble histone complexes in nuclei of Xenopus laevis oocytes.
1985,
Pubmed
,
Xenbase
Kozak,
Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles.
1983,
Pubmed
Krieg,
Formation of the 3' end of histone mRNA by post-transcriptional processing.
,
Pubmed
,
Xenbase
Kyte,
A simple method for displaying the hydropathic character of a protein.
1982,
Pubmed
Lahiri,
A cDNA clone of the hnRNP C proteins and its homology with the single-stranded DNA binding protein UP2.
1986,
Pubmed
Lapeyre,
Nucleolin, the major nucleolar protein of growing eukaryotic cells: an unusual protein structure revealed by the nucleotide sequence.
1987,
Pubmed
Lehrach,
RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.
1977,
Pubmed
Lothstein,
General organization of protein in HeLa 40S nuclear ribonucleoprotein particles.
1985,
Pubmed
Malcolm,
The structure of nuclear ribonucleoprotein of amphibian oocytes.
1977,
Pubmed
Martin,
Substructure of nuclear ribonucleoprotein complexes.
1978,
Pubmed
Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed
Mayrand,
Nuclear ribonucleoprotein particles probed in living cells.
1981,
Pubmed
McKnight,
Ultrastructural patterns of RNA synthesis during early embryogenesis of Drosophila melanogaster.
1976,
Pubmed
Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed
Merrill,
High pressure liquid chromatography purification of UP1 and UP2, two related single-stranded nucleic acid-binding proteins from calf thymus.
1986,
Pubmed
Messing,
New M13 vectors for cloning.
1983,
Pubmed
Nakagawa,
Molecular cloning of cDNA for the nuclear ribonucleoprotein particle C proteins: a conserved gene family.
1986,
Pubmed
Pederson,
Proteins associated with heterogeneous nuclear RNA in eukaryotic cells.
1974,
Pubmed
Rebagliati,
Identification and cloning of localized maternal RNAs from Xenopus eggs.
1985,
Pubmed
,
Xenbase
Sachs,
A single gene from yeast for both nuclear and cytoplasmic polyadenylate-binding proteins: domain structure and expression.
1986,
Pubmed
Sachs,
A single domain of yeast poly(A)-binding protein is necessary and sufficient for RNA binding and cell viability.
1987,
Pubmed
Samarina,
Reversible dissociation of nuclear ribonucleoprotein particle containing mRNA into RNA and protein.
1967,
Pubmed
Setyono,
Proteins associated with poly(A) and other regions of mRNA and hnRNA molecules as investigated by crosslinking.
1981,
Pubmed
Sillekens,
cDNA cloning of the human U1 snRNA-associated A protein: extensive homology between U1 and U2 snRNP-specific proteins.
1987,
Pubmed
Smith,
The nuclear location signal.
1985,
Pubmed
Swanson,
Primary structure of human nuclear ribonucleoprotein particle C proteins: conservation of sequence and domain structures in heterogeneous nuclear RNA, mRNA, and pre-rRNA-binding proteins.
1987,
Pubmed
Theissen,
Cloning of the human cDNA for the U1 RNA-associated 70K protein.
1986,
Pubmed
Van Eekelen,
Adenoviral heterogeneous nuclear RNA is associated with host cell proteins.
1981,
Pubmed
Wilk,
The core proteins of 35S hnRNP complexes. Characterization of nine different species.
1985,
Pubmed
Zasloff,
Impaired nuclear transport of a human variant tRNAiMet.
1982,
Pubmed
,
Xenbase
Zasloff,
tRNA transport from the nucleus in a eukaryotic cell: carrier-mediated translocation process.
1983,
Pubmed
,
Xenbase
de Vegvar,
3' end formation of U1 snRNA precursors is coupled to transcription from snRNA promoters.
1986,
Pubmed
van Eekelen,
Specificity in the interaction of hnRNA and mRNA with proteins as revealed by in vivo cross linking.
1981,
Pubmed
