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Methods Cell Biol
2015 Jan 01;127:131-59. doi: 10.1016/bs.mcb.2015.01.018.
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In vivo investigation of cilia structure and function using Xenopus.
Brooks ER
,
Wallingford JB
.
???displayArticle.abstract??? Cilia are key organelles in development and homeostasis. The ever-expanding complement of cilia associated proteins necessitates rapid and tractable models for in vivo functional investigation. Xenopus laevis provides an attractive model for such studies, having multiple ciliated populations, including primary and multiciliated tissues. The rapid external development of Xenopus and the large cells make it an especially excellent platform for imaging studies. Here we present embryological and cell biological methods for the investigation of cilia structure and function in X. laevis, with a focus on quantitative live and fixed imaging.
Blitz,
Biallelic genome modification in F(0) Xenopus tropicalis embryos using the CRISPR/Cas system.
2013, Pubmed,
Xenbase
Blitz,
Biallelic genome modification in F(0) Xenopus tropicalis embryos using the CRISPR/Cas system.
2013,
Pubmed
,
Xenbase
Boskovski,
The heterotaxy gene GALNT11 glycosylates Notch to orchestrate cilia type and laterality.
2013,
Pubmed
,
Xenbase
Bowes,
Xenbase: a Xenopus biology and genomics resource.
2008,
Pubmed
,
Xenbase
Brooks,
The Small GTPase Rsg1 is important for the cytoplasmic localization and axonemal dynamics of intraflagellar transport proteins.
2013,
Pubmed
,
Xenbase
Brooks,
Control of vertebrate intraflagellar transport by the planar cell polarity effector Fuz.
2012,
Pubmed
,
Xenbase
Chung,
RFX2 is broadly required for ciliogenesis during vertebrate development.
2012,
Pubmed
,
Xenbase
Chung,
Coordinated genomic control of ciliogenesis and cell movement by RFX2.
2014,
Pubmed
,
Xenbase
Cromey,
Avoiding twisted pixels: ethical guidelines for the appropriate use and manipulation of scientific digital images.
2010,
Pubmed
Dale,
Fate map for the 32-cell stage of Xenopus laevis.
1987,
Pubmed
,
Xenbase
Deblandre,
A two-step mechanism generates the spacing pattern of the ciliated cells in the skin of Xenopus embryos.
1999,
Pubmed
,
Xenbase
Eisen,
Controlling morpholino experiments: don't stop making antisense.
2008,
Pubmed
,
Xenbase
Fliegauf,
Mislocalization of DNAH5 and DNAH9 in respiratory cells from patients with primary ciliary dyskinesia.
2005,
Pubmed
Gray,
The planar cell polarity effector Fuz is essential for targeted membrane trafficking, ciliogenesis and mouse embryonic development.
2009,
Pubmed
,
Xenbase
Harland,
Xenopus research: metamorphosed by genetics and genomics.
2011,
Pubmed
,
Xenbase
Hayes,
Identification of novel ciliogenesis factors using a new in vivo model for mucociliary epithelial development.
2007,
Pubmed
,
Xenbase
Hellsten,
The genome of the Western clawed frog Xenopus tropicalis.
2010,
Pubmed
,
Xenbase
Heydeck,
Planar cell polarity effector gene Fuzzy regulates cilia formation and Hedgehog signal transduction in mouse.
2009,
Pubmed
Hoff,
ANKS6 is a central component of a nephronophthisis module linking NEK8 to INVS and NPHP3.
2013,
Pubmed
,
Xenbase
Ishikawa,
Ciliogenesis: building the cell's antenna.
2011,
Pubmed
James-Zorn,
Xenbase: expansion and updates of the Xenopus model organism database.
2013,
Pubmed
,
Xenbase
Joshi,
Microscopy tools for quantifying developmental dynamics in Xenopus embryos.
2012,
Pubmed
,
Xenbase
Keller,
Early embryonic development of Xenopus laevis.
1991,
Pubmed
,
Xenbase
Kieserman,
High-magnification in vivo imaging of Xenopus embryos for cell and developmental biology.
2010,
Pubmed
,
Xenbase
Kim,
Investigating morphogenesis in Xenopus embryos: imaging strategies, processing, and analysis.
2013,
Pubmed
,
Xenbase
Kim,
Planar cell polarity acts through septins to control collective cell movement and ciliogenesis.
2010,
Pubmed
,
Xenbase
Klos Dehring,
Deuterosome-mediated centriole biogenesis.
2013,
Pubmed
,
Xenbase
Kubo,
Sentan: a novel specific component of the apical structure of vertebrate motile cilia.
2008,
Pubmed
König,
Planar polarity in the ciliated epidermis of Xenopus embryos.
1993,
Pubmed
,
Xenbase
Lee,
Whole-mount fluorescence immunocytochemistry on Xenopus embryos.
2008,
Pubmed
,
Xenbase
Lyons,
The reproductive significance of human Fallopian tube cilia.
2006,
Pubmed
Mitchell,
The PCP pathway instructs the planar orientation of ciliated cells in the Xenopus larval skin.
2009,
Pubmed
,
Xenbase
Mitchell,
A positive feedback mechanism governs the polarity and motion of motile cilia.
2007,
Pubmed
,
Xenbase
Moody,
Fates of the blastomeres of the 32-cell-stage Xenopus embryo.
1987,
Pubmed
,
Xenbase
Park,
Dishevelled controls apical docking and planar polarization of basal bodies in ciliated epithelial cells.
2008,
Pubmed
,
Xenbase
Park,
Ciliogenesis defects in embryos lacking inturned or fuzzy function are associated with failure of planar cell polarity and Hedgehog signaling.
2006,
Pubmed
,
Xenbase
Pearl,
Development of Xenopus resource centers: the National Xenopus Resource and the European Xenopus Resource Center.
2012,
Pubmed
,
Xenbase
Pedersen,
Chlamydomonas IFT172 is encoded by FLA11, interacts with CrEB1, and regulates IFT at the flagellar tip.
2005,
Pubmed
Rosenbaum,
Intraflagellar transport.
2002,
Pubmed
Rossner,
What's in a picture? The temptation of image manipulation.
2004,
Pubmed
Satish Tammana,
Centrosomal protein CEP104 (Chlamydomonas FAP256) moves to the ciliary tip during ciliary assembly.
2013,
Pubmed
Sawamoto,
New neurons follow the flow of cerebrospinal fluid in the adult brain.
2006,
Pubmed
Schrøder,
EB1 and EB3 promote cilia biogenesis by several centrosome-related mechanisms.
2011,
Pubmed
Schweickert,
Cilia-driven leftward flow determines laterality in Xenopus.
2007,
Pubmed
,
Xenbase
Sharpey,
On a Peculiar Motion Excited in Fluids by the Surfaces of Certain Animals.
1830,
Pubmed
Shook,
Pattern and morphogenesis of presumptive superficial mesoderm in two closely related species, Xenopus laevis and Xenopus tropicalis.
2004,
Pubmed
,
Xenbase
Stubbs,
Multicilin promotes centriole assembly and ciliogenesis during multiciliate cell differentiation.
2012,
Pubmed
,
Xenbase
Stubbs,
Radial intercalation of ciliated cells during Xenopus skin development.
2006,
Pubmed
,
Xenbase
Suzuki,
High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos.
2013,
Pubmed
,
Xenbase
Tan,
Myb promotes centriole amplification and later steps of the multiciliogenesis program.
2013,
Pubmed
,
Xenbase
Tissir,
Lack of cadherins Celsr2 and Celsr3 impairs ependymal ciliogenesis, leading to fatal hydrocephalus.
2010,
Pubmed
Vincensini,
1001 model organisms to study cilia and flagella.
2011,
Pubmed
,
Xenbase
Vize,
Assays for gene function in developing Xenopus embryos.
1991,
Pubmed
,
Xenbase
Vladar,
Microtubules enable the planar cell polarity of airway cilia.
2012,
Pubmed
Wallingford,
Preparation of fixed Xenopus embryos for confocal imaging.
2010,
Pubmed
,
Xenbase
Wanner,
Mucociliary clearance in the airways.
1996,
Pubmed
Werner,
Understanding ciliated epithelia: the power of Xenopus.
2012,
Pubmed
,
Xenbase
Werner,
Actin and microtubules drive differential aspects of planar cell polarity in multiciliated cells.
2011,
Pubmed
,
Xenbase
Werner,
Using Xenopus skin to study cilia development and function.
2013,
Pubmed
,
Xenbase
Wessely,
Fish and frogs: models for vertebrate cilia signaling.
2008,
Pubmed
,
Xenbase
