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Development 2003 Jan 01;1301:71-83. doi: 10.1242/dev.00180.
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A single cdk inhibitor, p27Xic1, functions beyond cell cycle regulation to promote muscle differentiation in Xenopus.

Vernon AE , Philpott A .

The molecular basis of the antagonism between cellular proliferation and differentiation is poorly understood. We have investigated the role of the cyclin-dependent kinase inhibitor p27(Xic1) in the co-ordination of cell cycle exit and differentiation during early myogenesis in vivo using Xenopus embryos. In this report, we demonstrate that p27(Xic1) is highly expressed in the developing myotome, that ablation of p27(Xic1) protein prevents muscle differentiation and that p27(Xic1) synergizes with the transcription factor MyoD to promote muscle differentiation. Furthermore, the ability of p27(Xic1) to promote myogenesis resides in an N-terminal domain and is separable from its cell cycle regulation function. This data demonstrates that a single cyclin-dependent kinase inhibitor, p27(Xic1), controls in vivo muscle differentiation in Xenopus and that regulation of this process by p27(Xic1) requires activities beyond cell cycle inhibition.

PubMed ID: 12441292
Article link: Development

Species referenced: Xenopus laevis
Genes referenced: acta4 actl6a cdknx elavl1 gal.2 myf5 myh4 myh6 myod1 odc1 znrd2
Antibodies: Act3 Ab1 Cdknx Ab1 Myod1 Ab1
Morpholinos: cdknx MO1

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