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Dev Biol 2005 Sep 15;2852:570-83. doi: 10.1016/j.ydbio.2005.06.033.
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Identification of shared transcriptional targets for the proneural bHLH factors Xath5 and XNeuroD.

Logan MA , Steele MR , Van Raay TJ , Vetter ML .

Proneural basic helix-loop-helix (bHLH) transcription factors are critical positive regulators of neuronal differentiation in a variety of species and are required for proper differentiation of various subtypes of neurons. Although bHLH factors demonstrate some unique functions during neural development, they share the ability to regulate neuronal differentiation, potentially by targeting overlapping sets of genes. To assess this, we performed a screen in ectoderm animal cap tissue to identify direct transcriptional targets shared by two Xenopus ato-related bHLH factors, Xath5 and XNeuroD. Candidate target genes identified in this screen include several transcriptional regulators (Xebf2, Xebf3, XETOR and NKL), an RNA binding protein (elrC), a cell cycle component (Xgadd45gamma) and several novel genes. Overexpression of either Xath5 or XNeuroD induced ectopic in vivo expression of these candidate target genes. Conversely, blocking ato-related bHLH activity prevented endogenous nervous system expression of these genes. Therefore, we have identified a set of genes that can be regulated by multiple ato-related bHLH factors and may function as critical effectors of proneural bHLH-mediated differentiation.

PubMed ID: 16112102
Article link: Dev Biol
Grant support: [+]

Species referenced: Xenopus
Genes referenced: ascl2 atoh7 cbfa2t2 dll1 ebf2 ebf3 elavl3 gadd45g gal.2 glis2 nefm neurod1 neurod4 neurog2 sox2 svop tbxt tubb2b vxn

Article Images: [+] show captions