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J Biol Chem
2008 Jan 25;2834:1848-56. doi: 10.1074/jbc.M708044200.
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Two stage cadherin kinetics require multiple extracellular domains but not the cytoplasmic region.
Chien YH
,
Jiang N
,
Li F
,
Zhang F
,
Zhu C
,
Leckband D
.
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Micropipette manipulation measurements quantified the pre-steady state binding kinetics between cell pairs mediated by Xenopus cleavage stage cadherin. The time-dependence of the intercellular binding probability exhibits a fast forming, low probability binding state, which transitions to a slower forming, high probability state. The biphasic kinetics are independent of the cytoplasmic region, but the transition to the high probability state requires the third extracellular domain EC3. Deleting either EC3 or EC3-5, or substituting Trp(2) for Ala reduces the binding curves to a simple, monophasic rise in binding probability to a limiting plateau, as predicted for a single site binding mechanism. The two stage cadherin binding process reported here directly parallels previous biophysical studies, and confirms that the cadherin ectodomain governs the initial intercellular adhesion dynamics.
Ahrens,
Analysis of heterophilic and homophilic interactions of cadherins using the c-Jun/c-Fos dimerization domains.
2002, Pubmed
Ahrens,
Analysis of heterophilic and homophilic interactions of cadherins using the c-Jun/c-Fos dimerization domains.
2002,
Pubmed
Avizienyte,
Src-induced de-regulation of E-cadherin in colon cancer cells requires integrin signalling.
2002,
Pubmed
Bayas,
Lifetime measurements reveal kinetic differences between homophilic cadherin bonds.
2006,
Pubmed
,
Xenbase
Boggon,
C-cadherin ectodomain structure and implications for cell adhesion mechanisms.
2002,
Pubmed
,
Xenbase
Braga,
The challenges of abundance: epithelial junctions and small GTPase signalling.
2005,
Pubmed
Brieher,
Regulation of C-cadherin function during activin induced morphogenesis of Xenopus animal caps.
1994,
Pubmed
,
Xenbase
Brieher,
Lateral dimerization is required for the homophilic binding activity of C-cadherin.
1996,
Pubmed
,
Xenbase
Chappuis-Flament,
Multiple cadherin extracellular repeats mediate homophilic binding and adhesion.
2001,
Pubmed
,
Xenbase
Chesla,
Measuring two-dimensional receptor-ligand binding kinetics by micropipette.
1998,
Pubmed
Chesla,
The membrane anchor influences ligand binding two-dimensional kinetic rates and three-dimensional affinity of FcgammaRIII (CD16).
2000,
Pubmed
Dumaswala,
Glutamine- and phosphate-containing hypotonic storage media better maintain erythrocyte membrane physical properties.
1996,
Pubmed
Evans,
Detachment of agglutinin-bonded red blood cells. I. Forces to rupture molecular-point attachments.
1991,
Pubmed
Gold,
Chromic chloride: a coupling reagent for passive hemagglutination reactions.
1967,
Pubmed
Gumbiner,
Regulation of cadherin-mediated adhesion in morphogenesis.
2005,
Pubmed
Gumbiner,
Regulation of cadherin adhesive activity.
2000,
Pubmed
Harrison,
The mechanism of cell adhesion by classical cadherins: the role of domain 1.
2005,
Pubmed
He,
Untangling desmosomal knots with electron tomography.
2003,
Pubmed
,
Xenbase
Huang,
Kinetics of MHC-CD8 interaction at the T cell membrane.
2007,
Pubmed
Huang,
Quantifying the effects of molecular orientation and length on two-dimensional receptor-ligand binding kinetics.
2004,
Pubmed
Hynes,
Integrins: bidirectional, allosteric signaling machines.
2002,
Pubmed
Kaibuchi,
Regulation of cadherin-mediated cell-cell adhesion by the Rho family GTPases.
1999,
Pubmed
Kaplanski,
Granulocyte-endothelium initial adhesion. Analysis of transient binding events mediated by E-selectin in a laminar shear flow.
1993,
Pubmed
Koch,
Calcium binding and homoassociation of E-cadherin domains.
1997,
Pubmed
Kofler,
Some methodologic aspects of the chromium chloride method for coupling antigen to erythrocytes.
1977,
Pubmed
Long,
Kinetic measurements of cell surface E-selectin/carbohydrate ligand interactions.
2001,
Pubmed
Lu,
Locking in alternate conformations of the integrin alphaLbeta2 I domain with disulfide bonds reveals functional relationships among integrin domains.
2001,
Pubmed
Luo,
Disrupting integrin transmembrane domain heterodimerization increases ligand binding affinity, not valency or clustering.
2005,
Pubmed
Luo,
Locking the beta3 integrin I-like domain into high and low affinity conformations with disulfides.
2004,
Pubmed
Nose,
Expressed recombinant cadherins mediate cell sorting in model systems.
1988,
Pubmed
Nose,
Localization of specificity determining sites in cadherin cell adhesion molecules.
1990,
Pubmed
Ozawa,
Lateral dimerization of the E-cadherin extracellular domain is necessary but not sufficient for adhesive activity.
2002,
Pubmed
Panorchan,
Single-molecule analysis of cadherin-mediated cell-cell adhesion.
2006,
Pubmed
Patel,
Cadherin-mediated cell-cell adhesion: sticking together as a family.
2003,
Pubmed
Perret,
Trans-bonded pairs of E-cadherin exhibit a remarkable hierarchy of mechanical strengths.
2004,
Pubmed
Pertz,
A new crystal structure, Ca2+ dependence and mutational analysis reveal molecular details of E-cadherin homoassociation.
1999,
Pubmed
Pierres,
Measuring bonds between surface-associated molecules.
1996,
Pubmed
Pierres,
Measuring the lifetime of bonds made between surface-linked molecules.
1995,
Pubmed
Pokutta,
Conformational changes of the recombinant extracellular domain of E-cadherin upon calcium binding.
1994,
Pubmed
Prakasam,
Similarities between heterophilic and homophilic cadherin adhesion.
2006,
Pubmed
,
Xenbase
Prakasam,
Calcium site mutations in cadherin: impact on adhesion and evidence of cooperativity.
2006,
Pubmed
Shan,
Functional cis-heterodimers of N- and R-cadherins.
2000,
Pubmed
Shapiro,
Structural basis of cell-cell adhesion by cadherins.
1995,
Pubmed
Sivasankar,
Direct measurements of multiple adhesive alignments and unbinding trajectories between cadherin extracellular domains.
2001,
Pubmed
Sivasankar,
Direct molecular force measurements of multiple adhesive interactions between cadherin ectodomains.
1999,
Pubmed
,
Xenbase
Tomschy,
Homophilic adhesion of E-cadherin occurs by a co-operative two-step interaction of N-terminal domains.
1996,
Pubmed
Tsuiji,
Cadherin conformations associated with dimerization and adhesion.
2007,
Pubmed
,
Xenbase
Vorup-Jensen,
Structure and allosteric regulation of the alpha X beta 2 integrin I domain.
2003,
Pubmed
Wang,
Visualizing the mechanical activation of Src.
2005,
Pubmed
Weidner,
Scatter factor: molecular characteristics and effect on the invasiveness of epithelial cells.
1990,
Pubmed
Williams,
Concurrent binding to multiple ligands: kinetic rates of CD16b for membrane-bound IgG1 and IgG2.
2000,
Pubmed
Williams,
Quantifying the impact of membrane microtopology on effective two-dimensional affinity.
2001,
Pubmed
Williams,
Concurrent and independent binding of Fcgamma receptors IIa and IIIb to surface-bound IgG.
2000,
Pubmed
Yap,
Lateral clustering of the adhesive ectodomain: a fundamental determinant of cadherin function.
1997,
Pubmed
,
Xenbase
Yap,
Molecular and functional analysis of cadherin-based adherens junctions.
1997,
Pubmed
Yap,
The juxtamembrane region of the cadherin cytoplasmic tail supports lateral clustering, adhesive strengthening, and interaction with p120ctn.
1998,
Pubmed
,
Xenbase
Zhang,
Two-dimensional kinetics regulation of alphaLbeta2-ICAM-1 interaction by conformational changes of the alphaL-inserted domain.
2005,
Pubmed
Zhong,
Analysis of C-cadherin regulation during tissue morphogenesis with an activating antibody.
1999,
Pubmed
,
Xenbase
Zhu,
Functional analysis of the structural basis of homophilic cadherin adhesion.
2003,
Pubmed
Zhu,
Modeling concurrent binding of multiple molecular species in cell adhesion.
2000,
Pubmed
