Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Lin XH, Walter J, Scheidtmann K, Ohst K, Newport J, Walter G.
???displayArticle.abstract???
Protein phosphatase 2A (PP2A) is an abundant, multifunctional serine/threonine-specific phosphatase that stimulates simian virus 40 DNA replication. The question as to whether chromosomal DNA replication also depends on PP2A was addressed by using a cell-free replication system derived from Xenopus laevis eggs. Immunodepletion of PP2A from Xenopus egg extract resulted in strong inhibition of DNA replication. PP2A was required for the initiation of replication but not for the elongation of previously engaged replication forks. Therefore, the initiation of chromosomal DNA replication depends not only on phosphorylation by protein kinases but also on dephosphorylation by PP2A.
Adachi,
Study of the cell cycle-dependent assembly of the DNA pre-replication centres in Xenopus egg extracts.
1994, Pubmed,
Xenbase
Adachi,
Study of the cell cycle-dependent assembly of the DNA pre-replication centres in Xenopus egg extracts.
1994,
Pubmed
,
Xenbase Blow,
Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.
1986,
Pubmed
,
Xenbase Bosch,
The PR55 and PR65 subunits of protein phosphatase 2A from Xenopus laevis. molecular cloning and developmental regulation of expression.
1995,
Pubmed
,
Xenbase Cegielska,
Different oligomeric forms of protein phosphatase 2A activate and inhibit simian virus 40 DNA replication.
1994,
Pubmed Coleman,
The Xenopus Cdc6 protein is essential for the initiation of a single round of DNA replication in cell-free extracts.
1996,
Pubmed
,
Xenbase Csortos,
High complexity in the expression of the B' subunit of protein phosphatase 2A0. Evidence for the existence of at least seven novel isoforms.
1996,
Pubmed Diffley,
Once and only once upon a time: specifying and regulating origins of DNA replication in eukaryotic cells.
1996,
Pubmed Dutta,
Initiation of DNA replication in eukaryotic cells.
1997,
Pubmed Hardy,
mcm5/cdc46-bob1 bypasses the requirement for the S phase activator Cdc7p.
1997,
Pubmed Healy,
CDC55, a Saccharomyces cerevisiae gene involved in cellular morphogenesis: identification, characterization, and homology to the B subunit of mammalian type 2A protein phosphatase.
1991,
Pubmed Hendrix,
Structure and expression of a 72-kDa regulatory subunit of protein phosphatase 2A. Evidence for different size forms produced by alternative splicing.
1993,
Pubmed Hua,
A role for Cdk2 kinase in negatively regulating DNA replication during S phase of the cell cycle.
1997,
Pubmed
,
Xenbase Kinoshita,
Distinct, essential roles of type 1 and 2A protein phosphatases in the control of the fission yeast cell division cycle.
1990,
Pubmed Klausing,
Effects of in vitro dephosphorylation on DNA-binding and DNA helicase activities of simian virus 40 large tumor antigen.
1988,
Pubmed Kremmer,
Separation of PP2A core enzyme and holoenzyme with monoclonal antibodies against the regulatory A subunit: abundant expression of both forms in cells.
1997,
Pubmed Lee,
The role of protein phosphatase type-2A in the Xenopus cell cycle: initiation of the G2/M transition.
1995,
Pubmed
,
Xenbase Lee,
Inhibition of cdc2 activation by INH/PP2A.
1994,
Pubmed
,
Xenbase Lei,
Mcm2 is a target of regulation by Cdc7-Dbf4 during the initiation of DNA synthesis.
1997,
Pubmed Lin,
The role of Saccharomyces cerevisiae type 2A phosphatase in the actin cytoskeleton and in entry into mitosis.
1995,
Pubmed Mayer,
Structure of the 55-kDa regulatory subunit of protein phosphatase 2A: evidence for a neuronal-specific isoform.
1991,
Pubmed McCright,
The B56 family of protein phosphatase 2A (PP2A) regulatory subunits encodes differentiation-induced phosphoproteins that target PP2A to both nucleus and cytoplasm.
1996,
Pubmed McCright,
Identification of a new family of protein phosphatase 2A regulatory subunits.
1995,
Pubmed McVey,
Phosphorylation of large tumour antigen by cdc2 stimulates SV40 DNA replication.
1989,
Pubmed Minshull,
Protein phosphatase 2A regulates MPF activity and sister chromatid cohesion in budding yeast.
1996,
Pubmed Mohr,
Regulation of SV40 DNA replication by phosphorylation of T antigen.
1987,
Pubmed Mumby,
Protein serine/threonine phosphatases: structure, regulation, and functions in cell growth.
1993,
Pubmed Murphy,
The role of protein phosphorylation in the assembly of a replication competent nucleus: investigations in Xenopus egg extracts using the cyanobacterial toxin microcystin-LR.
1995,
Pubmed
,
Xenbase Newport,
Nuclear reconstitution in vitro: stages of assembly around protein-free DNA.
1987,
Pubmed
,
Xenbase Pallas,
The third subunit of protein phosphatase 2A (PP2A), a 55-kilodalton protein which is apparently substituted for by T antigens in complexes with the 36- and 63-kilodalton PP2A subunits, bears little resemblance to T antigens.
1992,
Pubmed Romanowski,
The Xenopus origin recognition complex is essential for DNA replication and MCM binding to chromatin.
1996,
Pubmed
,
Xenbase Scheidtmann,
Protein phosphatase 2A dephosphorylates simian virus 40 large T antigen specifically at residues involved in regulation of DNA-binding activity.
1991,
Pubmed Scheidtmann,
Simian virus 40 large T antigen is phosphorylated at multiple sites clustered in two separate regions.
1982,
Pubmed Simmons,
Phosphorylation downregulates the DNA-binding activity of simian virus 40 T antigen.
1986,
Pubmed Tanabe,
Molecular cloning of a 74-kDa regulatory subunit (B" or delta) of human protein phosphatase 2A.
1996,
Pubmed Tehrani,
Identification of a novel protein phosphatase 2A regulatory subunit highly expressed in muscle.
1996,
Pubmed Virshup,
Mechanism of activation of simian virus 40 DNA replication by protein phosphatase 2A.
1992,
Pubmed Virshup,
Activation of SV40 DNA replication in vitro by cellular protein phosphatase 2A.
1989,
Pubmed Virshup,
Purification of replication protein C, a cellular protein involved in the initial stages of simian virus 40 DNA replication in vitro.
1989,
Pubmed Walter,
Regulation of replicon size in Xenopus egg extracts.
1997,
Pubmed
,
Xenbase Walter,
Protein serine/threonine phosphatases and cell transformation.
1993,
Pubmed Walter,
Regulated chromosomal DNA replication in the absence of a nucleus.
1998,
Pubmed
,
Xenbase Zolnierowicz,
Diversity in the regulatory B-subunits of protein phosphatase 2A: identification of a novel isoform highly expressed in brain.
1994,
Pubmed Zou,
Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin.
1998,
Pubmed
