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Eur Biophys J
1990 Jan 01;186:327-33. doi: 10.1007/bf00196923.
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Divalent cations as probes for structure-function relationships of cloned voltage-dependent sodium channels.
Pusch M
.
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1. Several cloned sodium channels were expressed in oocytes and compared with respect to their sensitivity to internal Mg2+ concerning the open-channel block and to external Ca2+ concerning open-channel block and shifts in steady-state activation. 2. A quantitative comparison between wild-type II channels and a mutant with a positive charge in the S4 segment of repeat I neutralized (K226Q) revealed no significant differences in the Mg2+ block. 3. The blocking effect of extracellular Ca2+ ions on single-channel inward currents was studied for type II, mutant K226Q and type III. A quantitative comparison showed that all three channel types differ significantly in their Ca2+ sensitivity. 4. The influence of extracellular Ca2+ on the voltage dependence of steady-state activation of macroscopic currents was compared for type II and K226Q channels. Extracellular Ca2+ increases the voltage of half-maximal activation, V1/2, more for K226Q than for wild-type II channels; a plot of V1/2 against [Ca]o is twice as steep for the mutant K226Q as for the wild-type on a logarithmic concentration scale. 5. The differential effects of extracellular Ca2+ and intracellular Mg2+ on wild-type II and K226Q channels are discussed in terms of structural models of the Na+ channel protein.
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Begenisich,
Sodium channel permeation in squid axons. II: Non-independence and current-voltage relations.
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Begenisich,
Sodium channel permeation in squid axons. I: Reversal potential experiments.
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Catterall,
Molecular properties of voltage-sensitive sodium channels.
1986,
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Dani,
Ion-channel entrances influence permeation. Net charge, size, shape, and binding considerations.
1986,
Pubmed
FRANKENHAEUSER,
The action of calcium on the electrical properties of squid axons.
1957,
Pubmed
Gilly,
Slowing of sodium channel opening kinetics in squid axon by extracellular zinc.
1982,
Pubmed
HODGKIN,
A quantitative description of membrane current and its application to conduction and excitation in nerve.
1952,
Pubmed
Hahin,
Simple shifts in the voltage dependence of sodium channel gating caused by divalent cations.
1983,
Pubmed
Hille,
Negative surface charge near sodium channels of nerve: divalent ions, monovalent ions, and pH.
1975,
Pubmed
Kayano,
Primary structure of rat brain sodium channel III deduced from the cDNA sequence.
1988,
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Nilius,
Calcium block of guinea-pig heart sodium channels with and without modification by the piperazinylindole DPI 201-106.
1988,
Pubmed
Noda,
Existence of distinct sodium channel messenger RNAs in rat brain.
,
Pubmed
Provencher,
A Fourier method for the analysis of exponential decay curves.
1976,
Pubmed
Pusch,
Open-channel block of Na+ channels by intracellular Mg2+.
1990,
Pubmed
,
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Stühmer,
Patch clamp characterization of sodium channels expressed from rat brain cDNA.
1987,
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,
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Stühmer,
Structural parts involved in activation and inactivation of the sodium channel.
1989,
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,
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Vandenberg,
Single-channel, macroscopic, and gating currents from sodium channels in the squid giant axon.
1991,
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Woodhull,
Ionic blockage of sodium channels in nerve.
1973,
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Worley,
Trimethyloxonium modification of single batrachotoxin-activated sodium channels in planar bilayers. Changes in unit conductance and in block by saxitoxin and calcium.
1986,
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Yamamoto,
Voltage-dependent calcium block of normal and tetramethrin-modified single sodium channels.
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