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Kinetochores are the proteinaceous complexes that assemble on centromeric DNA and direct eukaryotic chromosome segregation. The mechanisms by which higher eukaryotic cells define centromeres are poorly understood. Possible molecular contributors to centromere specification include the underlying DNA sequences and epigenetic factors such as binding of the centromeric histone centromere protein A (CENP-A). Frog egg extracts are an attractive system for studying centromere definition and kinetochore assembly. To facilitate such studies, we cloned a Xenopus laevis homologue of CENP-A (XCENP-A). We identified centromere-associated DNA sequences by cloning fragments of DNA that copurified with XCENP-A by chromatin immunoprecipitation. XCENP-A associates with frog centromeric repeat 1 (Fcr1), a 174-base pair repeat containing a possible CENP-B box. Southern blots of partially digested genomic DNA revealed large ordered arrays of Fcr1 in the genome. Fluorescent in situ hybridization with Fcr1 probes stained most centromeres in cultured cells. By staining lampbrush chromosomes, we specifically identified the 11 (of 18) chromosomes that stain consistently with Fcr1 probes.
Alonso,
Genomic microarray analysis reveals distinct locations for the CENP-A binding domains in three human chromosome 13q32 neocentromeres.
2003, Pubmed
Alonso,
Genomic microarray analysis reveals distinct locations for the CENP-A binding domains in three human chromosome 13q32 neocentromeres.
2003,
Pubmed Amor,
Human centromere repositioning "in progress".
2004,
Pubmed Amor,
Neocentromeres: role in human disease, evolution, and centromere study.
2002,
Pubmed Ando,
CENP-A, -B, and -C chromatin complex that contains the I-type alpha-satellite array constitutes the prekinetochore in HeLa cells.
2002,
Pubmed Biggins,
Captivating capture: how microtubules attach to kinetochores.
2003,
Pubmed Bloom,
The centromere frontier: kinetochore components, microtubule-based motility, and the CEN-value paradox.
1993,
Pubmed Callan,
Lampbrush chromosomes.
1986,
Pubmed Carbon,
Structural and functional analysis of a yeast centromere (CEN3).
1984,
Pubmed Clarke,
Centromeres: proteins, protein complexes, and repeated domains at centromeres of simple eukaryotes.
1998,
Pubmed Cleveland,
Centromeres and kinetochores: from epigenetics to mitotic checkpoint signaling.
2003,
Pubmed Csink,
Something from nothing: the evolution and utility of satellite repeats.
1998,
Pubmed Greig,
Epistasis and hybrid sterility in Saccharomyces.
2002,
Pubmed Henikoff,
The centromere paradox: stable inheritance with rapidly evolving DNA.
2001,
Pubmed Houben,
DNA and proteins of plant centromeres.
2003,
Pubmed Howman,
Early disruption of centromeric chromatin organization in centromere protein A (Cenpa) null mice.
2000,
Pubmed Hummel,
Characterization of highly and moderately repetitive 500 bp Eco RI fragments from Xenopus laevis DNA.
1984,
Pubmed
,
Xenbase Jamrich,
Transcription of repetitive sequences on Xenopus lampbrush chromosomes.
1983,
Pubmed
,
Xenbase Keith,
CSE4 genetically interacts with the Saccharomyces cerevisiae centromere DNA elements CDE I and CDE II but not CDE III. Implications for the path of the centromere dna around a cse4p variant nucleosome.
2000,
Pubmed Kinoshita,
The identification of two novel ligands of the FGF receptor by a yeast screening method and their activity in Xenopus development.
1995,
Pubmed
,
Xenbase Lam,
Tandemly repeated DNA sequences from Xenopus laevis. I. Studies on sequence organization and variation in satellite 1 DNA (741 base-pair repeat).
1983,
Pubmed
,
Xenbase Lo,
A novel chromatin immunoprecipitation and array (CIA) analysis identifies a 460-kb CENP-A-binding neocentromere DNA.
2001,
Pubmed Malik,
Adaptive evolution of Cid, a centromere-specific histone in Drosophila.
2001,
Pubmed Masumoto,
A human centromere antigen (CENP-B) interacts with a short specific sequence in alphoid DNA, a human centromeric satellite.
1989,
Pubmed Mellone,
Stretching it: putting the CEN(P-A) in centromere.
2003,
Pubmed Murphy,
RNA polymerase III in Cajal bodies and lampbrush chromosomes of the Xenopus oocyte nucleus.
2002,
Pubmed
,
Xenbase Nagaki,
Chromatin immunoprecipitation reveals that the 180-bp satellite repeat is the key functional DNA element of Arabidopsis thaliana centromeres.
2003,
Pubmed Oegema,
Functional analysis of kinetochore assembly in Caenorhabditis elegans.
2001,
Pubmed Ohzeki,
CENP-B box is required for de novo centromere chromatin assembly on human alphoid DNA.
2002,
Pubmed Palmer,
Purification of the centromere-specific protein CENP-A and demonstration that it is a distinctive histone.
1991,
Pubmed Panzeri,
Role of conserved sequence elements in yeast centromere DNA.
1985,
Pubmed Ragghianti,
Molecular characterization of a centromeric satellite DNA in the hemiclonal hybrid frog Rana esculenta and its parental species.
1995,
Pubmed
,
Xenbase Ronquist,
MrBayes 3: Bayesian phylogenetic inference under mixed models.
2003,
Pubmed Rovira,
A repetitive DNA sequence associated with the centromeres of Chironomus pallidivittatus.
1993,
Pubmed Smith,
Centromeres and variant histones: what, where, when and why?
2002,
Pubmed Sullivan,
Stable dicentric X chromosomes with two functional centromeres.
1998,
Pubmed Sun,
Molecular structure of a functional Drosophila centromere.
1997,
Pubmed Thompson,
The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.
1997,
Pubmed Van Hooser,
Methods for in situ localization of proteins and DNA in the centromere-kinetochore complex.
1999,
Pubmed Warburton,
Epigenetic analysis of kinetochore assembly on variant human centromeres.
2001,
Pubmed Warburton,
Immunolocalization of CENP-A suggests a distinct nucleosome structure at the inner kinetochore plate of active centromeres.
1997,
Pubmed Witkiewicz,
Improved design of riboprobes from pBluescript and related vectors for in situ hybridization.
1993,
Pubmed Wood,
CENP-E is a plus end-directed kinetochore motor required for metaphase chromosome alignment.
1997,
Pubmed
,
Xenbase Yoda,
Human centromere protein A (CENP-A) can replace histone H3 in nucleosome reconstitution in vitro.
2000,
Pubmed
