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J Biol Chem
2011 Dec 09;28649:42221-42231. doi: 10.1074/jbc.M111.303677.
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Protein arginine methyltransferase Prmt5-Mep50 methylates histones H2A and H4 and the histone chaperone nucleoplasmin in Xenopus laevis eggs.
Wilczek C
,
Chitta R
,
Woo E
,
Shabanowitz J
,
Chait BT
,
Hunt DF
,
Shechter D
.
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Histone proteins carry information contained in post-translational modifications. Eukaryotic cells utilize this histone code to regulate the usage of the underlying DNA. In the maturing oocytes and eggs of the frog Xenopus laevis, histones are synthesized in bulk in preparation for deposition during the rapid early developmental cell cycles. During this key developmental time frame, embryonic pluripotent chromatin is established. In the egg, non-chromatin-bound histones are complexed with storage chaperone proteins, including nucleoplasmin. Here we describe the identification and characterization of a complex of the protein arginine methyltransferase 5 (Prmt5) and the methylosome protein 50 (Mep50) isolated from Xenopus eggs that specifically methylates predeposition histones H2A/H2A.X-F and H4 and the histone chaperone nucleoplasmin on a conserved motif (GRGXK). We demonstrate that nucleoplasmin (Npm), an exceedingly abundant maternally deposited protein, is a potent substrate for Prmt5-Mep50 and is monomethylated and symmetrically dimethylated at Arg-187. Furthermore, Npm modulates Prmt5-Mep50 activity directed toward histones, consistent with a regulatory role for Npm in vivo. We show that H2A and nucleoplasmin methylation appears late in oogenesis and is most abundant in the laid egg. We hypothesize that these very abundant arginine methylations are constrained to pre-mid blastula transition events in the embryo and therefore may be involved in the global transcriptional repression found in this developmental time frame.
Aggarwal,
Nuclear cyclin D1/CDK4 kinase regulates CUL4 expression and triggers neoplastic growth via activation of the PRMT5 methyltransferase.
2010, Pubmed
Aggarwal,
Nuclear cyclin D1/CDK4 kinase regulates CUL4 expression and triggers neoplastic growth via activation of the PRMT5 methyltransferase.
2010,
Pubmed
Akey,
Histone chaperones and nucleosome assembly.
2003,
Pubmed
Ancelin,
Blimp1 associates with Prmt5 and directs histone arginine methylation in mouse germ cells.
2006,
Pubmed
Anizet,
Characterization of a new cell line, XL2, obtained from Xenopus laevis and determination of optimal culture conditions.
1981,
Pubmed
,
Xenbase
Arnan,
Interaction of nucleoplasmin with core histones.
2003,
Pubmed
,
Xenbase
Ausió,
Histone variants--the structure behind the function.
2006,
Pubmed
Banaszynski,
Analysis of histones and chromatin in Xenopus laevis egg and oocyte extracts.
2010,
Pubmed
,
Xenbase
Barski,
High-resolution profiling of histone methylations in the human genome.
2007,
Pubmed
Bañuelos,
Phosphorylation of both nucleoplasmin domains is required for activation of its chromatin decondensation activity.
2007,
Pubmed
,
Xenbase
Bedford,
Arginine methylation an emerging regulator of protein function.
2005,
Pubmed
Bedford,
Arginine methylation at a glance.
2007,
Pubmed
Bedford,
Protein arginine methylation in mammals: who, what, and why.
2009,
Pubmed
Bernstein,
The mammalian epigenome.
2007,
Pubmed
Bürglin,
Cloning of nucleoplasmin from Xenopus laevis oocytes and analysis of its developmental expression.
1987,
Pubmed
,
Xenbase
Cheng,
Small molecule regulators of protein arginine methyltransferases.
2004,
Pubmed
Cotten,
Massive phosphorylation distinguishes Xenopus laevis nucleoplasmin isolated from oocytes or unfertilized eggs.
1986,
Pubmed
,
Xenbase
Eitoku,
Histone chaperones: 30 years from isolation to elucidation of the mechanisms of nucleosome assembly and disassembly.
2008,
Pubmed
Friesen,
The methylosome, a 20S complex containing JBP1 and pICln, produces dimethylarginine-modified Sm proteins.
2001,
Pubmed
Friesen,
A novel WD repeat protein component of the methylosome binds Sm proteins.
2002,
Pubmed
Furuno,
Association of Polycomb group SUZ12 with WD-repeat protein MEP50 that binds to histone H2A selectively in vitro.
2006,
Pubmed
Garcia,
Chemical derivatization of histones for facilitated analysis by mass spectrometry.
2007,
Pubmed
Kalinich,
Rapid isolation of nuclear transport-competent Xenopus nucleoplasmin produced in Escherichia coli strain BL21(DE3).
1994,
Pubmed
,
Xenbase
Kouzarides,
Chromatin modifications and their function.
2007,
Pubmed
Le Guezennec,
MBD2/NuRD and MBD3/NuRD, two distinct complexes with different biochemical and functional properties.
2006,
Pubmed
Lee,
Minireview: protein arginine methylation of nonhistone proteins in transcriptional regulation.
2009,
Pubmed
Leno,
Hyperphosphorylation of nucleoplasmin facilitates Xenopus sperm decondensation at fertilization.
1996,
Pubmed
,
Xenbase
Liu,
JAK2V617F-mediated phosphorylation of PRMT5 downregulates its methyltransferase activity and promotes myeloproliferation.
2011,
Pubmed
Luger,
Expression and purification of recombinant histones and nucleosome reconstitution.
1999,
Pubmed
Luger,
Crystal structure of the nucleosome core particle at 2.8 A resolution.
1997,
Pubmed
Nagamatsu,
A germ cell-specific gene, Prmt5, works in somatic cell reprogramming.
2011,
Pubmed
Newport,
A major developmental transition in early Xenopus embryos: II. Control of the onset of transcription.
1982,
Pubmed
,
Xenbase
Nicklay,
Analysis of histones in Xenopus laevis. II. mass spectrometry reveals an index of cell type-specific modifications on H3 and H4.
2009,
Pubmed
,
Xenbase
Ramos,
Nucleoplasmin binds histone H2A-H2B dimers through its distal face.
2010,
Pubmed
,
Xenbase
Rho,
Prmt5, which forms distinct homo-oligomers, is a member of the protein-arginine methyltransferase family.
2001,
Pubmed
Sealy,
Xenopus nucleoplasmin: egg vs. oocyte.
1986,
Pubmed
,
Xenbase
Sealy,
Purification of Xenopus egg nucleoplasmin and its use in chromatin assembly in vitro.
1989,
Pubmed
,
Xenbase
Shafa,
Returning to the stem state: epigenetics of recapitulating pre-differentiation chromatin structure.
2010,
Pubmed
Shechter,
MCM proteins and checkpoint kinases get together at the fork.
2004,
Pubmed
,
Xenbase
Shechter,
Analysis of histones in Xenopus laevis. I. A distinct index of enriched variants and modifications exists in each cell type and is remodeled during developmental transitions.
2009,
Pubmed
,
Xenbase
Shechter,
A distinct H2A.X isoform is enriched in Xenopus laevis eggs and early embryos and is phosphorylated in the absence of a checkpoint.
2009,
Pubmed
,
Xenbase
Sible,
Developmental regulation of MCM replication factors in Xenopus laevis.
1998,
Pubmed
,
Xenbase
Stols,
A new vector for high-throughput, ligation-independent cloning encoding a tobacco etch virus protease cleavage site.
2002,
Pubmed
Strahl,
The language of covalent histone modifications.
2000,
Pubmed
Tamada,
Chromatin decondensation and nuclear reprogramming by nucleoplasmin.
2006,
Pubmed
,
Xenbase
Taneva,
Activation of nucleoplasmin, an oligomeric histone chaperone, challenges its stability.
2008,
Pubmed
Tee,
Prmt5 is essential for early mouse development and acts in the cytoplasm to maintain ES cell pluripotency.
2010,
Pubmed
Turner,
Defining an epigenetic code.
2007,
Pubmed
Wang,
Human PAD4 regulates histone arginine methylation levels via demethylimination.
2004,
Pubmed
Wolf,
The protein arginine methyltransferase family: an update about function, new perspectives and the physiological role in humans.
2009,
Pubmed
Woodcock,
Role of linker histone in chromatin structure and function: H1 stoichiometry and nucleosome repeat length.
2006,
Pubmed
Wyrick,
The role of histone H2A and H2B post-translational modifications in transcription: a genomic perspective.
2009,
Pubmed
Wysocka,
Histone arginine methylation and its dynamic regulation.
2006,
Pubmed
Yamada,
DNA replication checkpoint control of Wee1 stability by vertebrate Hsl7.
2004,
Pubmed
,
Xenbase
Yanai,
Mapping gene expression in two Xenopus species: evolutionary constraints and developmental flexibility.
2011,
Pubmed
,
Xenbase
