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Science
2017 Jan 20;3556322:. doi: 10.1126/science.aaa3761.
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Distortion of histone octamer core promotes nucleosome mobilization by a chromatin remodeler.
Sinha KK
,
Gross JD
,
Narlikar GJ
.
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Adenosine 5'-triphosphate (ATP)-dependent chromatin remodeling enzymes play essential biological roles by mobilizing nucleosomal DNA. Yet, how DNA is mobilized despite the steric constraints placed by the histone octamer remains unknown. Using methyl transverse relaxation-optimized nuclear magnetic resonance spectroscopy on a 450-kilodalton complex, we show that the chromatin remodeler, SNF2h, distorts the histone octamer. Binding of SNF2h in an activated ATP state changes the dynamics of buried histone residues. Preventing octamer distortion by site-specific disulfide linkages inhibits nucleosome sliding by SNF2h while promoting octamer eviction by the SWI-SNF complex, RSC. Our findings indicate that the histone core of a nucleosome is more plastic than previously imagined and that octamer deformation plays different roles based on the type of chromatin remodeler. Octamer plasticity may contribute to chromatin regulation beyond ATP-dependent remodeling.
Aalfs,
Functional differences between the human ATP-dependent nucleosome remodeling proteins BRG1 and SNF2H.
2001, Pubmed
Aalfs,
Functional differences between the human ATP-dependent nucleosome remodeling proteins BRG1 and SNF2H.
2001,
Pubmed
Alilat,
Nucleosome dynamics. Protein and DNA contributions in the chiral transition of the tetrasome, the histone (H3-H4)2 tetramer-DNA particle.
1999,
Pubmed
Andrews,
Nucleosome structure(s) and stability: variations on a theme.
2011,
Pubmed
Bazett-Jones,
The SWI/SNF complex creates loop domains in DNA and polynucleosome arrays and can disrupt DNA-histone contacts within these domains.
1999,
Pubmed
Blosser,
Dynamics of nucleosome remodelling by individual ACF complexes.
2009,
Pubmed
Boyer,
Roles of the histone H2A-H2B dimers and the (H3-H4)(2) tetramer in nucleosome remodeling by the SWI-SNF complex.
2000,
Pubmed
Brehove,
Histone core phosphorylation regulates DNA accessibility.
2015,
Pubmed
Bruno,
Histone H2A/H2B dimer exchange by ATP-dependent chromatin remodeling activities.
2003,
Pubmed
,
Xenbase
Cavallo,
POPS: A fast algorithm for solvent accessible surface areas at atomic and residue level.
2003,
Pubmed
Clapier,
A critical epitope for substrate recognition by the nucleosome remodeling ATPase ISWI.
2002,
Pubmed
Clapier,
Critical role for the histone H4 N terminus in nucleosome remodeling by ISWI.
2001,
Pubmed
,
Xenbase
Clapier,
The biology of chromatin remodeling complexes.
2009,
Pubmed
Clapier,
Regulation of DNA Translocation Efficiency within the Chromatin Remodeler RSC/Sth1 Potentiates Nucleosome Sliding and Ejection.
2016,
Pubmed
Corona,
ISWI is an ATP-dependent nucleosome remodeling factor.
1999,
Pubmed
Cosgrove,
Regulated nucleosome mobility and the histone code.
2004,
Pubmed
Côté,
Perturbation of nucleosome core structure by the SWI/SNF complex persists after its detachment, enhancing subsequent transcription factor binding.
1998,
Pubmed
Dang,
Domain architecture of the catalytic subunit in the ISW2-nucleosome complex.
2007,
Pubmed
Dang,
Regulation of ISW2 by concerted action of histone H4 tail and extranucleosomal DNA.
2006,
Pubmed
Davey,
Solvent mediated interactions in the structure of the nucleosome core particle at 1.9 a resolution.
2002,
Pubmed
,
Xenbase
Deindl,
ISWI remodelers slide nucleosomes with coordinated multi-base-pair entry steps and single-base-pair exit steps.
2013,
Pubmed
Falk,
Chromosomes. CENP-C reshapes and stabilizes CENP-A nucleosomes at the centromere.
2015,
Pubmed
Fan,
Distinct strategies to make nucleosomal DNA accessible.
2003,
Pubmed
Fei,
The prenucleosome, a stable conformational isomer of the nucleosome.
2015,
Pubmed
Flaus,
Unlocking the nucleosome.
2017,
Pubmed
Flaus,
Sin mutations alter inherent nucleosome mobility.
2004,
Pubmed
Gangaraju,
Conformational changes associated with template commitment in ATP-dependent chromatin remodeling by ISW2.
2009,
Pubmed
Guyon,
Stability of a human SWI-SNF remodeled nucleosomal array.
2001,
Pubmed
Hamel,
The contact interface of a 120 kD CheA-CheW complex by methyl TROSY interaction spectroscopy.
2005,
Pubmed
Hamiche,
Histone tails modulate nucleosome mobility and regulate ATP-dependent nucleosome sliding by NURF.
2001,
Pubmed
Harada,
Stepwise nucleosome translocation by RSC remodeling complexes.
2016,
Pubmed
Ho,
Chromatin remodelling during development.
2010,
Pubmed
Horigome,
SWR1 and INO80 chromatin remodelers contribute to DNA double-strand break perinuclear anchorage site choice.
2014,
Pubmed
Horn,
The SIN domain of the histone octamer is essential for intramolecular folding of nucleosomal arrays.
2002,
Pubmed
,
Xenbase
Hota,
Nucleosome mobilization by ISW2 requires the concerted action of the ATPase and SLIDE domains.
2013,
Pubmed
Imbalzano,
Nucleosome disruption by human SWI/SNF is maintained in the absence of continued ATP hydrolysis.
1996,
Pubmed
Ito,
ACF, an ISWI-containing and ATP-utilizing chromatin assembly and remodeling factor.
1997,
Pubmed
Kagalwala,
Topography of the ISW2-nucleosome complex: insights into nucleosome spacing and chromatin remodeling.
2004,
Pubmed
Kato,
Architecture of the high mobility group nucleosomal protein 2-nucleosome complex as revealed by methyl-based NMR.
2011,
Pubmed
Kruger,
Amino acid substitutions in the structured domains of histones H3 and H4 partially relieve the requirement of the yeast SWI/SNF complex for transcription.
1995,
Pubmed
Leonard,
A nucleotide-driven switch regulates flanking DNA length sensing by a dimeric chromatin remodeler.
2015,
Pubmed
Lia,
Direct observation of DNA distortion by the RSC complex.
2006,
Pubmed
Lorch,
Activated RSC-nucleosome complex and persistently altered form of the nucleosome.
1998,
Pubmed
Luger,
Crystal structure of the nucleosome core particle at 2.8 A resolution.
1997,
Pubmed
Mittermaier,
New tools provide new insights in NMR studies of protein dynamics.
2006,
Pubmed
Morrison,
Chromatin remodelling beyond transcription: the INO80 and SWR1 complexes.
2009,
Pubmed
Muthurajan,
Crystal structures of histone Sin mutant nucleosomes reveal altered protein-DNA interactions.
2004,
Pubmed
Narlikar,
Mechanisms and functions of ATP-dependent chromatin-remodeling enzymes.
2013,
Pubmed
Narlikar,
Cooperation between complexes that regulate chromatin structure and transcription.
2002,
Pubmed
Prior,
Reversible changes in nucleosome structure and histone H3 accessibility in transcriptionally active and inactive states of rDNA chromatin.
1983,
Pubmed
Racki,
The histone H4 tail regulates the conformation of the ATP-binding pocket in the SNF2h chromatin remodeling enzyme.
2014,
Pubmed
Racki,
The chromatin remodeller ACF acts as a dimeric motor to space nucleosomes.
2009,
Pubmed
Rosenzweig,
Bringing dynamic molecular machines into focus by methyl-TROSY NMR.
2014,
Pubmed
Saha,
Chromatin remodeling by RSC involves ATP-dependent DNA translocation.
2002,
Pubmed
Schlosshauer,
Realistic protein-protein association rates from a simple diffusional model neglecting long-range interactions, free energy barriers, and landscape ruggedness.
2004,
Pubmed
Schnitzler,
Human SWI/SNF interconverts a nucleosome between its base state and a stable remodeled state.
1998,
Pubmed
Shen,
A chromatin remodelling complex involved in transcription and DNA processing.
2000,
Pubmed
Sprangers,
Quantitative dynamics and binding studies of the 20S proteasome by NMR.
2007,
Pubmed
Sprangers,
Quantitative NMR spectroscopy of supramolecular complexes: dynamic side pores in ClpP are important for product release.
2005,
Pubmed
Tosi,
Structure and subunit topology of the INO80 chromatin remodeler and its nucleosome complex.
2013,
Pubmed
Tsukiyama,
Purification and properties of an ATP-dependent nucleosome remodeling factor.
1995,
Pubmed
Tugarinov,
Cross-correlated relaxation enhanced 1H[bond]13C NMR spectroscopy of methyl groups in very high molecular weight proteins and protein complexes.
2003,
Pubmed
Udugama,
The INO80 ATP-dependent chromatin remodeling complex is a nucleosome spacing factor.
2011,
Pubmed
Whitehouse,
Evidence for DNA translocation by the ISWI chromatin-remodeling enzyme.
2003,
Pubmed
Zaret,
Pioneer transcription factors: establishing competence for gene expression.
2011,
Pubmed
Zhang,
DNA translocation and loop formation mechanism of chromatin remodeling by SWI/SNF and RSC.
2006,
Pubmed
Zhou,
Mechanisms of ATP-Dependent Chromatin Remodeling Motors.
2016,
Pubmed
Zofall,
Chromatin remodeling by ISW2 and SWI/SNF requires DNA translocation inside the nucleosome.
2006,
Pubmed
