Return to search results Title: E2a is necessary for Smad2/3 dependent transcription and the direct repression of lefty

Summary

We characterized the binding of Smad2/3 using ChIP-SEQ in both control gastrula-stage X. tropicalis embryos and embryos depleted of the transcription factor E2a (E2a-MO). We also characterized gene expression in control and E2a-depleted embryos by RNA-Seq. For ChIP-Seq, three biological replicates of E2a-MO were performed, and two biological replicates of Control embryos were performed. For RNA-Seq, two biological replicates of E2a-MO were performed, and two biological replicates of Control embryos were performed, and the mean expression levels of each gene were compared.

Contributors: Andrea Wills, Andrea Wills, Julie Baker

Experiment Type: For ChIP-Seq, three biological replicates were performed for E2a-depleted X. tropicalis embryos, and two biological replicates were performed for control gastrula-stage embryos. For RNA-Seq, two biological replicates were performed for both E2a-depleted embryos and control embryos, and the mean expression levels were compared for each gene.

Experiment Reagents: tcf3 MO1.


Article: XB-ART-50519, PubMed

Source: NCBI GEO, Xenbase Download

Sample	View		GSMs	Assay Type
WE - NF10.5 GSM1357039  GSM1357040  RNA-Seq Xtr   [-] [+] Background: Xtr.Outbred Conditions: Tissue Assay Stages ChIP Antibody embryo NF stage 10.5      WE + tcf3 MO - NF10.5 DEG GSM1357041  GSM1357042  RNA-Seq Xtr   [-] [+] Background: Xtr.Outbred Manipulations: Manipulation Type Reagent Target Gene Stage Treatment Area morpholino injection tcf3 MO1 tcf3 NF stage 2 (2-cell) blastomere Conditions: Tissue Assay Stages ChIP Antibody embryo NF stage 10.5   input WE - NF10.5 GSM1357037  GSM1357038  ChIP-Seq [-] [+] Background: Xtr.Outbred Conditions: Tissue Assay Stages ChIP Antibody embryo NF stage 10.5      Smad2/3 WE + tcf3 MO - NF10.5 GSM1357034  GSM1357035  GSM1357036  ChIP-Seq Xtr   [-] [+] Background: Xtr.Outbred Manipulations: Manipulation Type Reagent Target Gene Stage Treatment Area morpholino injection tcf3 MO1 tcf3 NF stage 2 (2-cell) blastomere Conditions: Tissue Assay Stages ChIP Antibody embryo NF stage 10.5 Smad2 Ab9    Smad2/3 WE - NF10.5 GSM1357032  GSM1357033  ChIP-Seq Xtr   [-] [+] Background: Xtr.Outbred Conditions: Tissue Assay Stages ChIP Antibody embryo NF stage 10.5 Smad2 Ab9

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