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Summary Genes Attributions

Library Name: NIH_XGC_tropMet3

Species: Xenopus tropicalis

Number Of Clones: 189

Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pSPORT1 vector. This library contains the 0.5-1.5 kb size fraction (the 1.5-2 kb fraction is contained in the NIH_XGC_tropMet2 library).Library constructed at the DOE Joint Genome Institute.

Normalized: No Subtraction: No
Anatomy: whole organism Stage: NF stage 62

Vector DetailsVector Map
Name: pSPORT1

Type: phagemid

5' Restriction Site: SalI

3' Restriction Site: NotI
Expression Image