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Summary Genes Attributions

Library Name: RIKEN Xenopus egg

Species: Xenopus laevis

Number Of Clones: 2365

Description: cDNA oligo-dT primed using oligo 5'-GAGAGAGAGAGGATCCAATACTGGAGAGTTTTTTTTTTTTTTTTVN-3' and directionally cloned into XhoI and BamHI sites using the 5' adaptor 5'- AGAGAGAGAGCTCGAGCTCTAATAAGGTGACACTATAGAACCA-3'. Size selection was done automatically by the cloning vector (lambda FLC-II, P. Carninci et al., unpublished). Library was amplified at the phage stage and normalized (RoT value = 5). This library was constructed using the CapTrapper method and Trehalose thermoactivated reverse transcriptase to enrich for full-length clones. Library constructed by Piero Carninci (RIKEN, Genome Science Laboratory, Japan: using egg RNA supplied by Marc Kirschner (Harvard Medical School, Dept. of Cell Biology). References: Methods Enzymol.303:19-44 and Genome Res. 10:1617-1630 Please contact Marc Kirschner ( for information on obtaining aliquots of this cDNA library.

Normalized: No Subtraction: No
Anatomy: egg Stage:

Vector DetailsVector Map
Name: pCS2G

Type: plasmid

5' Restriction Site: XhoI

3' Restriction Site: BamHI
Expression Image