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XB-LIB-67
Library Name: NICHD_XGC_tropLimb_m |
Species: Xenopus tropicalis |
Number Of Clones: 8514 |
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.
| Normalized: No | Subtraction: No |
| Anatomy: limb | Stage: NF stage 55 to NF stage 66 |
| Vector Details | Vector Map | Name: pCS107 Type: plasmid 5' Restriction Site: SalI 3' Restriction Site: NotI |  |