XB-CLONE-8596722
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Clone Name: wnt11-816
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Gene:
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Species: Xenopus laevis
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Description: EXRC Clone Number 816.
Xenopus laevis maternal protein (wnt-11) mRNA, complete cds.
To generate probe for in situ and mRNA for injection.
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Sequence:
Full length sequence: Wnt11 (R Mayor)_FullLength
[+]
angcgTTGGgncTCTCcgGaTccaGCTTaTCGatTTCGAnCCCGGGGTACCGAATTCCGAaaaATATAACATTTACAAATATAAAAAAAAAaCACAACGt
TGTAAGACTTCGTGTCTCATTGTCTAAAAAGATTTTTTTTTTtGTTTTTTACAGATAAAAACGTCCAGAATACTCTGAACTTCAAATATCCAGTTATACA
CATATACATACATACCCACATATACAGTGGGGGACTTTTAAAACAAGTAGTAACTGAAATATGAATGAGTCCCAGATTCAGATTTCAAGTAAGAGTTGAG
CCTCATCTTTATAGTCCAATTTAGGCATATTAAAAGTTCATATTGAAAAAAAAAaCAGAATTAAACCCCCAAAAACTGGCCCCtgAAACAGAATTAAGGA
TCCAGCATTTGCCAGCATATTGCAAATGCAATATGGGAATTAATAAATAGGCTCAGTCTGGTGCTGCCAGTTGTTACCCAGTCTATTTACAGACACCAGA
GGTATCACTTGGGTTCCTTGACCTGGCAGGCAATAAATCCGTTGTTGTTACTGGCTTTAGGACTTCAGGATGGCTTGGCTGTTTCACGTCAAAGGGGGGA
ACCTATGCATATTTTGTGTTTGGTCCTTAGAGTTTCTTTGAGCCACTTGGGGCCCATACATTGACGGGCAAGCAGATCAGACCAGTTGCATTTGGCTTCC
CCTAGCTTTAATCCAGAAGAAAAGTTAAAACGTTCCAGCTCTGCTTCTCCCTTTGCCAGGGAAGGTCTGTTTGCTGAACCACAGAGGGCTGTCAGTGCTT
TAGTAGTGTACAGTGTGAAGTCCATTATGGCTTTACTTGCAGACATACCTCTCCACTGTCCGCTCACATTTCTTGCACATGACATAGCAGCACCAGTGGT
ACTTGCATTGACAGCGTTCCACGATGGTTTCGGTGTAGgCGTTGTATCCCCGCCCACAGCACATGaggTTGCAGCTGTCACTACcaaCgGAGGTCTTGTT
GCACAGCCTGTCCTGTGTCCCATATGAAcCCAACttgGGattCtttgngCAGT
Construction:
Insert:
Vector:| Vector Details | Vector Map |
Name: pGEM-7Zf-
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: null |  |
Usage:
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RNA injection:
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Probes:
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| Type |
Size |
Promoter |
Linearization Site |
Description |
| sense |
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T7 |
SacI |
ClaI can also be used |
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Whole mount in situ hybridization:
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VBI
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Probes:
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| Type |
Size |
Promoter |
Linearization Site |
Description |
| antisense |
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SP6 |
XbaI |
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Suppliers:
Search for clone at:
EXRC
