Summary Attributions

Clone Name: otx2-HAR29	Gene: otx2	Species: Xenopus tropicalis
Description: EXRC Clone Number Har-29. TGas025f18. Xenopus tropicalis orthodenticle homeobox 2. 5' present. To generate probe for in situ.

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Sequence:

5' EST: AL629928 [+]

ctggggactttgcgtcaaaaaattaccggagaggcgccttctctctgcaaaggttccagggacaaggagacacgtcagatccgagcagctccagcctttc
ctcccctgaagcccctgtgtatggcactgacagccgaggactccgcatccccctcctgaaaaagcctccccatagacttgcctttgttatttaacccgat
tctctcggtttattgcaggagaggaggaatccggctcggccgaggatcatttgctccttatttgttaagaccattgtgtgcaaaggaagagcgggatcaa
gaagcacacactacactgatcgcccgacttgctgcaacgattttctgattttctttcgcggattgtgctccctgataaaacactagcatgatgtcttatc
tcaagcaaccgccatatgccgtgaatgggctcagcctgactacctccgggatggatttgttacatccgtcggtgggatatcccgttgcgctacaccagag
ccgccagataacgtgtagtttctatgattttgcagcgacccccaggaaacagaggagggaaaggaccactttcaccagagcccaactggatgtcctcgaa
gcactttttgccaaaactcgttaccccgatatcttcatgagagaagaagtggctctaaaaatcaacttgccggagtccagagtccangtttggttcaaaa
accgccgagccaagtgccggcagcagcaacagcagcagcagaatggggg

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Library:

Name: XGC-gastrula

External Dbs: Unigene tropicalis cDNA library

Description: cDNA was oligo dT primed from 5ug of poly A+ RNA from stages 10-13 gastrulae. EcoRI-NotI cut cDNA was then ligated into pCS107 with EcoRI at the 5' end and NotI at the 3' end.

Anatomy: whole organism	Stage: NF stage 10.5 to NF stage 12
Normalized: No	Subtraction: No

Vector Details	Vector Map
Name: pCS107 Type: plasmid 5' Restriction Site: EcoRI 3' Restriction Site: NotI

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Usage:

Whole mount in situ hybridization:
Probes:
Type Size Promoter Linearization Site Description antisense T7 EcoRI

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Suppliers:

Search for clone at: EXRC