XB-CLONE-3152581
Clone Name: pax8-HAR131
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Gene:
pax8
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Species: Xenopus tropicalis
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Description: EXRC Clone Number HAR-131. IMAGE:7863442.
Xenopus tropicalis paired box 8. Full length. Accession number DT412463.
To generate probe for in situ. Approximately
950bp of x.trop pax8 was amplified using the following primers:
ggaattccGGTCACGGAGGTTTGAATCAGTTAG
cgggatcccgTGGGGCTACATAAAGATGAAGGG
which
also places an EcoRI site at the 5'end and a BamHI site at the 3'end.
This was amplfiied using Pfu from 25-30 stage x.trop cDNA
Amplicon was cloned into the RI-BamHI site of pBS KS II.
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Sequence:
Full length sequence: BC125805 [+]
CTAAGAGCTCATCACAGAGAGGGGCTGCTCAGGACCTGCCAGCAATCTCAATATAGGTCACGGAGGTTTGAATCAGCTAGGTGGTGCCTTTGTGAATGGG
CGACCCCTGCCAGAGGTGGTGAGGCAGCGAATAGTTGACCTGGCACATCAGGGGGTACGTCCCTGTGACATCTCACGACAGCTCCGAGTCAGTCATGGCT
GTGTCAGCAAAATTTTGGGCAGGTACTATGAGACAGGCAGTATTCGGCCAGGCGTCATCGGAGGTTCCAAGCCCAAGGTTGCCACCCCCAAAGTGGTGGA
GAAAATCGGAGATTACAAACGCCAGAACCCTACAATGTTTGCCTGGGAAATCAGGGACCGGCTGCTGGCAGAGGGGGTGTGCGACAATGACACAGTTCCC
AGCGTCAGCTCTATCAACAGAATCATACGCACCAAAGTACAGCAACTTTTTAACCTGCCTATGGAGAGCTGCGTAAAATCACTGAGCCCAGGACAGACTT
TAATCCCTAGTTCAGCAGTCACGCCCCCTGAGTCTCCCCATTCAGACTCTCTGGGATCCACGTATTCCATCAGCGGTTTGTTGGGAATCACGCAGCCCGG
CGCGGATGGAAAGCGCAAACTTGATGACAGTGACCAGGAGAGCTGCAGGCTCAGCATTGACTCCCAAGGCAGCATGGGGATCAGTAGGAAACAGCTGCGC
ACCGAGGCATATGGGCACCACCCTCTGGACGCCCTGGAGTGTCACTTCCAGAGACAACATTTCCCAGAGTCCTACTCCTCTTCCACCCACAGCAAAACAG
AACAGGCTCTATATACCCTACCCCTGCTCAACAATGCAATGGATGATGGGAAATCTTCACTGACTTCCACAAACACAACAATTGGTAGAAACCTCTCGAC
CCACCAGGGATACTCTGCTCTATCTGAACTCTCAGCCTTCACCATTAAACAAGAGGCTTCAGACAGCTCCAGCGCTAGCTCCACCCCTTCATCTTTATGT
AGCCCCACCTTCCTGGATCTGCAGCCAATCAGCTCAGGATGCTCAGCGCCATCCTTCAGCGCTTTTTCACACCCCGCATCTGTGTACGGCCAGTTCACAA
GCCACATGGCCTCAGGTCGTGATGTGGTTGGATCCACGTTGCCAGGGTACCCACCTCACATACCTAGCGGGCAAGGAAATTACGCCTCTTCTGCGATTGC
TGGAATGGTAGCTGCAGGAGGTGATTACTCTGGAAATGCCTATAGCCATGGGGCTTATGCAGCCTACGGAGAGAGTTGGCGTTTCCCCAGCTCAAGCCTT
CTAGGCTCTCCCTATTACTACAGCTCTGCCACAAGGACTGCACCTCCTCCCACAACGGCAGGAGCCTATGACCTTCTGTAGTCTTCATGGAGACCGTTGA
GTAGCATCATCAACAAAGCAACCCGGCCCTGGGAAAGTGGCTTAATGGTGGACAAAACTAACCATGGCAACACATGGTCACAAGTGTTATGAGACAACAA
GTAACAAGAACAACATATCAAACATGGACTAAAGTTACCCTTTTAATCTTGGACTTCCTGGAGCTGTGGATGATATTCGGTAACGCAAGAGATGAAACCT
CCGAAGAAGATACAAAGAAAAATCACATTCATTGTTTCTGCAAGCCTAACAAGGAATATTTTTTTGTTCTCTCCACAAATGTAGAAAATGTATAGAGCCA
TGTACAAGGGTGTTACATACATTTATATATTGGGGTATATAACAAAATATTTGCTAGGCATTTCAGCTGCGATGTGTAGTTAGCCTAACAATGTGTAGTT
TCTCCTGTTGATCCTAAGGACAATGGTAAAGACGGCCAGGGTCAGACTGGGCTGCCAGAGTACCACAGGATCTCTAAATGGGCCCAGGATGATTCCCTTC
AGTCCTTTCTTATTTTCACCATAGGTTTAGCACAGTCTACATGGAATTGCTGTGCGCCCTCCTTGTCAGGTTCTCCTATGTTCCCTAGGTGACCCAATTT
GCAAATTCATTGCAACTCTTGCAGCTCTGCAAATGGAGCTTCTTAATCTGTTCTCAAACTTCCAACAACCTGCAGTGGATGCTGGGCAATTTAGTTTAAC
AACAGATGGAGCAAGAGAACACTGTAGTACAAGGATTTCCAACTGCACTGTTCTGTACATTCAGAGCTGAGCAAGTGCTGGACCTGGCAGGAAACAAACC
AACTGCAGCCCATTCGTCCCCTCTCCGTAACATCCCCAAATTAAAATGGTGAAGTAAAACACCAAAAAGAATGTTGGCAGGAAACACTACACATTGTTTA
AAGGTAATCACCCTGAACTGCCCTTGATTTACCTTTCTCTGTAACACAAAAAAAAATGAAGTAAGCATTCAGCTGCTCCTCTCATCTGCCCTCTTTGGTT
TAAAAGTTTAATAACTGTCCCTTATTTATATGGAGTTGGCTCTGGGGTCTGACAGCAAAAAACTTCCTCCCTATTGGTGGAAGTGCCTTCTGGATAAACG
GAGGCAGAAAAGAGTTGTATGAGGTGGGCCCGCAGGCTGCTGTTCTCATCCCAATGCCGTGTTCCGTGGCATGTTTACAAATGCAGCAATATTTTATCCA
GTACGAGGAGGGAACATAGAACATAAACCCCCTTACCCTTTCCTCTCACTTAAGAATAAGCAATGCACATATCCATTAATTTAGTAGAGTAACTATAGGA
TACCTGGCCCCATATCACAATCTTTTTAGGGCCCCTTAAGCTTTGGGAATAAGATACAATATACCACTTTATCAGTTAGTGGCCCACTGGGCCCCCTAAA
TAAATTGCCATTCAGCATTTGGATTTTATTACTGTCCACTTTTAAAAGAAATAATTTTTTTGAAAGCAGAATTTTCCCAAGGCTGCCCAATGAACACACT
CAAAGCATCCCTGCTGGGAAGGGAGTACAAGTTGGGTTTGTAATGCTGCATTTAAGTAAGTGAGGAGGGGAAATGCTGGAGTTGTTGTACTTCCACTCCC
TGCACAGCTGCAGGAGCAAAGACTGCACAAATAGACTCAAAACTGTATATAAATATATGTATGTACAAAATATACATCTATGCAGAGCTCTGTCTCTATA
TATATATACTGTTTTTATAGCCACAAGGGCTGTACCAGTGACTGGAATGCACTAGTTATTCATACTCTTAGTGCCGGTTCACAATCACTCTAGAAATCTG
ACTTGGAAGAAGATGCAAATCACACAGCGGGGCTAATTTACTAAGATAGGTGCTAAATTGCCCCTGAGTGTAATCACCCATGGCAACCAGCCAACAATTA
ACTGTGATCTGCCCACTTATGTTAGAAATGAAAGATCTAATTGGTGGTTACTGGGACACGTTAATACCTATGTCAGTGAGTCAGCCCCATGACTGTGTGT
TAACCAGTTGGGCCAAAGGCTATGAATTAACTTCATTTTGATTGGGACATCAACCTGCAAGTGTTTCTAATTACTTGTGCCAAGGCATAGACATAAGTTA
TATTCTGTTTGCCCTTCCTGTTTTCGACGTGGCGGCTCAGTGCACACAAACGCTGGGGAATATAATGCAAGAGGTTACAATGAAGGAAAGACTTAAGATC
AGGCATTATTCCAGGATTGTGTCTGCTCCGCACTAAATGACTTTATCACATGGCCCTGTCTGTGGGAATACAGTAACTGCAGGGCCAGACTGTAAATAAA
TATTTGTAATATAAAATATTTTAAATAAATAAGCATGAAGGAAAAAAAAAAAAAAAAAA
5' EST: DT412463 [+]ctaagagctcatcacagagaggggctgctcaggacctgccagcaatctcaatataggtcacggaggtttgaatcagctaggtggtgcctttgtgaatggg
cgacccctgccagaggtggtgaggcagcgaatagttgacctggcacatcagggggtacgtccctgtgacatctcacgacagctccgagtcagtcatggct
gtgtcagcaaaattttgggcaggtactatgagacaggcagtattcggccaggcgtcatcggaggttccaagcccaaggttgccacccccaaagtggtgga
gaaaatcggagattacaaacgccagaaccctacaatgtttgcctgggaaatcagggaccggctgctggcagagggggtgtgcgacaatgacacagttccc
agcgtcagctctatcaacagaatcatacgcaccaaagtacagcaactttttaacctgcctatggagagctgcgtaaaatcactgagcccaggacagactt
taatccctagttcagcagtcacgccccctgagtctccccattcagactctctgggatccacgtattccatcagcggtttgttgggaatcacgcagcccgg
cgcggatggaaagcgcaaacttgatgacagtgaccaggagagctgcaggctcagcattgactcccaaggcagcatggggatcagtaggaaacagctgcgc
accgaggcatatgggcaccaccctctggacgccctggagtgtcacttccagagacaacatttcccagagtcctactcctc
3' EST: DT412462 [+]ccttcatgcttatttatttaaaatattttatattacaaatatttatttacagtctggccctgcagttactgtattcccacagacagggccatgtgataaa
gtcatttagtgcggagcagacacaatcctggaataatgcctgatcttaagtctttccttcattgtaacctcttgcattatattccccagcgtttgtgtgc
actgagccgccacgtcgaaaacaggaagggcaaacagaatataacttatgtctatgccttggcacaagtaattagaaacacttgcaggttgatgtcccaa
tcaaaatgaagttaattcatagcctttggcccaactggttaacacacagtcatggggctgactcactgacataggtattaacgtgtcccagtaaccacca
attagatctttcatttctaacataagtgggcagatcacagttaattgttggctggttgccatgggtgattacactcaggggcaatttagcacctatctta
gtaaattagccccgctgtgtgatttgcatcttcttccaagtcagatttctagagtgattgtgaaccggcactaagagtatgaataactagtgcattccag
tcactggtacagcccttgtggctataaaaacagtatatatatatagagacagagctctgcatagatgtatattttgtacatacatatatttatatacagt
tttgagtctatttgtgcagtctttgctcctgcagctgtgcagggagtggaagtacaacaactccagcatttcccctcctcacttacttaaatgcagcatt
acaaaccca
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1745 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: oviduct | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Whole mount in situ hybridization:
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Probes:
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Type |
Size |
Promoter |
Linearization Site |
Description |
antisense |
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T7 |
EcoRI |
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Suppliers:
Search for clone at:
EXRC