Summary Attributions

Clone Name: id4-775	Gene: id4.S	Species: Xenopus laevis
Description: EXRC Clone Number 775. IMAGE:3580163. Xenopus laevis inhibitor of DNA binding 4, dominant negative helix-loop-helix protein (id4-a), mRNA. To generate probe for in situ.

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Sequence:

5' EST: BG160187 [+]

gagctgggaggttgtatgtccagctgactgataggatcctgctgtccctgccattattatctgcagagctgccttcctgtcattccgcctgagtgccctg
ttatcccgagcaaacagctgctttgtagcagccacaacgagcctaataaggaattgcatttgccaagcagttggtgcccccttatctagaaagaagcggc
acctgtatcttcccccagtgtctctgcgttggattgacagtcgcatggtctatttctgttgctgttcagtcgcacggtagacagtcgctgactcacacac
acacacgaggctccaggtatctgctgccatgaaagctgtcagtccagtgcgcccgcagagtcggaaagcccaagtgccgtctgtgtgcggggagctggcg
ctgcattgcctgtctgagcacagcctcggggtagctcgctacaagatggaagaagaggagaccctgtgcctgcagtatgatatgaatgactgttacagcc
ggctcaagagactcgtgcccaccattccacccaacaagaaagtcagcaaagtggaaatcctgcagcatgttattgactatatcttggatctgcagttagc
actagacacacacccagtgctgctcaagcaacagtcaccaacccagacccctctt

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Library:

Name: Wellcome CRC pRN3 head

External Dbs: Unigene laevis cDNA library, Xenopus IMAGE cDNA Library

Description: cDNAs were oligo-dT primed and directionally cloned. Staging according to Nieuwkoop and Faber. Library was constructed by N. Garrett, K. Ryan and A.M. Zorn, (Wellcome/CRC Institute).

Anatomy: head	Stage: NF stage 29 and 30
Normalized: No	Subtraction: No

Vector Details	Vector Map
Name: pBSRN3 Type: phagemid 5' Restriction Site: EcoRI 3' Restriction Site: NotI

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Usage:

Whole mount in situ hybridization:	Expressed on Cement gland, somites, VBI, head and HSC.
Probes:
Type Size Promoter Linearization Site Description antisense T7 EcoRI BgIII can also be used.

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Suppliers:

Search for clone at: EXRC