Summary Attributions

Clone Name: gjc1-850	Gene: gjc1.L	Species: Xenopus laevis
Description: EXRC Clone Number 850. gap junction protein, gamma 1, 45kDa. Image EST 4683883, Accession BC042343 (full length). To generate probe for in situ.

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Sequence:

Full length sequence: BC042343 [+]

GGCGCTTCTATTGCCCCAGCGGAGAGATCGCGCGGAACCTGGAGCCGCTAGTACAGTAGTAAGTGTCCTGCTGAGTGCTAACCGACTGCGCTTATACGCT
CCTTTACTCCTCTCAGTACAGTGAGAACTAGCGCTATTACTAGAGCTGATCGGGGCTCAGAATGAGCTGGAGTTTCTTAACCCGTCTCCTTGAGGAGATC
AACAACCACTCAACATTCGTTGGGAAGGTCTGGCTGACTGTGCTCATCATATTCCGAATTGTCCTGACTGCTGTTGGTGGGGAGTCTATATATTATGATG
AACAGAGTAAATTTTCGTGCAACACGCAGCAGCCCGGTTGCGAGAATGTCTGCTATGACAAATTTGCGCCCCTGTCCCACGTTCGATTCTGGGTTTTCCA
GATTATCCTCATCACCACCCCATCCATTATGTATCTGGGGTTCGCCATGCATCGGATTGCACGTGAGCCAGAGGAGAGGCAAAGGCGCATGGATAAAACA
AAGAGCAAAAAGCGCGCTCCCATAATTCATCGGGGAGCCATGAGAGACTATGAAGAGGCTGAAGATAATCAAGAGGAAGACCCCATGATTTGTGAAGAAG
AGGAACATGAAAAAGACAGTGACAAAGGAGACAAAAAGAAACACGATGGCCGCCGGCGAATCAAACAAGACGGCTTGATGAAAGTCTACGTGCTGCAACT
GCTGGTCCGTTCAGTGTTTGAAGTTGGCTTCCTGATGGGCCAGTATATATTATATGGGTTTGAGGTCATTCCCTACTTTGTGTGCACCCGGAAACCATGC
CCTCATACTGTCGACTGCTTTGTGTCCCGTCCTACTGAGAAGACCATCTTTCTGCTGATCATGTATGCTGTCAGCGCACTCTGCCTGTTCCTGAACCTGT
GCGAGCTCTTCCACTTAGGCATAGGGGGTATCCGAGATGCCCTTCGACAGAAGAGAAAAGAAATTCAGGAATCCCGTAAGAAAGCCCCAAGTGCCCCTCC
AAATTACCACTCCGTACTGAAGAAAGGCAGACTGCCCAACGGCAAACCAGTTTTTCCAGGAAACGGAGTGTCTGAAGGCTTTGAGCTGCCTGTACATGAG
CTGGACAGGTTGAGGCAGCACCTCAAGTTGGCTCAAGAGCACCTTGACTTGGCTTTCCACCTGAACCCAACAGGCGATACTGCAAATGCTTCCCGAAGTA
GTAGCCCTGAGGCCAACAGCATTGCTGCAGAACAGAATCGGCTGAACCTTGCACAAGAGAAGGGAGCGGGAAGCCGTGAAAAATCTGGTTTGTGATCGTG
GAGAGCGGGTGGGTCGTGGGGGGGGGGAATGGAATGGCTTGTTGTTCAACTGGAATCAAGTTACTAAAAGGTTCTTGACCCTCTCCTGGCAGGGGGGGAA
GCGCGGAGGGCGAGAGGAAGTGAAGAAAAGGGAACTATTTTTTTTTGGGGGGGGGGGGTTGTTGGGGGAAGGGTAGGAGGTTTGAATGCACCTCTGTGTT
TTGTGAACTGATCATGTTCTTTGTCTCATGCCAAGTGCTGCCAATGCCGGGCGAGGTTTAATACCACTGGTTTACATAGGCTGGCGGCTGTTCTACCCTC
TGGTTATAGGGTGAGAGCCCATTTCAGCCTAGAAATATTTTTATTTGAATTTGATATTTTTGTGTACTGGAAGCTGCTGCAAACACTTGTATTGTGCTGC
AGACTGTAATGCCGCATGTGTTGGAATAGTTCAACTATTTCAGCGTTGGACAACCTTGCGGCTGTAGGTTGCCAGCTTACTTTCAACGCAATTCTTTTAT
ACGATACCTAGCATAAATGAAAGGGATATCTTGCCTTTTTATTTTTGTGCTTTTATGTTTTTTTTTTTGTTTTTAGATGTAGTTCCATATGATTTTGCTG
CGGTACTCTGAGCCCATCAGTGCCAGCACACCTTCCCTCTGACTGTATTTGTTTTGGAGGGGATTTATAAGCTTTATGCATTCCTGGGTTCTGTTAAAGT
TCTGATCCTCCCTGCTGTTCGCCTGTTGAACTAACTACAAGTCCCAGCATCCGATGACAGGCTGAGGGTTCTCATTCAGAAACAGCTGGAGGGTTGGAGG
GAAAAATTCCATGTTACTATTTTTTTTGTCTTTTTGCATTTGACAGATTTTGTAAGGAAAACTTGGCACCAGAGCTGGAGGATGGGGTTGTGCCAAATTC
ATTCACGATTAGGGGAAGGGGAGGCTAATTTTTCCATTCTCCCCTCTCCCCTGTTTTTTGAAAGCTTTAAACATTCCAGAGGCGCTCCTTCTGCACACAT
GTTATGTACATGGAGTGGTACAGCCCACGGGGCACAACAGTATATCAAGAACTAGAGAATGTGCCTTTCCCGTTTGCAGAAGGGAGTTGGGTTTGCTGAA
TTGTTTTTGCTGTTTAATGGGGCACTTCAGTCTGTTCCTGGTGCTTCCAACTGCTTTAGGGTAACCTGCACATTCCATCAGTCACCACCCGCTCCTTCAC
AAATTCCAAAAAAATAACCTCACGTGTCTAGGAGCCGGGTGAGGGGGCTACTGTGTATACAGAGGTGAACAGCTGTTTGAAATACTTGCATTAACCCATT
GGTTGATGGTATTTTGGCCAATGGAAACCAGCTGCGTTATAGTAAATCACACCTCCCTCTGTCACCTCCGTGCCTTTAAAGATAAAATACAAGTTTCTAT
GCCAGAGCACCCTGTTACTTTATGTACTAACATTTCCATTTCCAAAACATTCTTATAACTGGAACTATTTACACTGCTCTTTCCCACTAGGGGGTGCCAT
TACCTTGATGTACCTAATCTCAAGCACCAAAGCATTCTCTCCTAGAACTTTCTTCTTCAATGGAAAATTAACCCATGCCTGCACTTGCAAATAGCTCCAT
ATATTGTTTCCTTATGTGACAAAGAAAGGAGAAATTATTTTATTTTAATGTGACTTTTTAGTTAGTTATTTGAAGTGTTATTCTCTGTCATGGTAGCATT
CCTGGGTTCTAAGTTTGGGCAGAAGAATTCGAAACAAGGCACATGCCGTTTTTTCACTCTTCTATGGAAAAGGAGCATCAAAACCAGGTAATTGTTACAG
ATCTTTGACTAAAGCAAGGGACGAAAGCACGCCAAATTGATGGCCTTCTTCCGGAAACAATCCTATACGTGCATCATTCAGGGATTCACAGCTTCCCTGT
GTTTTGACACTGCTTTTCCATAGAAAAATGACGTGCCCCTTCGGATGTAGTCTCCACGGTAACAGTGTGCTCTTTCATAGGAAGCCATTTTCCATCTTGT
ATTTTATTCTTCTTTATCTACGCTGGAGACGCTTGAAATTGTACAGACATCTCATAGCTTCATCTCTTTATATGATAGGATATTTGTCTTTATTGGGGGG
GGGGCTGTATGGTTATGTTTTATTGAAGACTTCTTGGTACTGGAAACATTATTTTGTTTTTTATACAGTGTTATTAAAAAAAAAAAAAAAA

5' EST: BQ736450 [+]

cgcttctattgccccagcggagagatcgcgcggaacctggagccgctagtacagtagtaagtgtcctgctgagtgctaaccgactgcgcttatacgctcc
tttactcctctcagtacagtgagaactagcgctattactagagctgatcggggctcagaatgagctggagtttcttaacccgtctccttgaggagatcaa
caaccactcaacattcgttgggaaggtctggctgactgtgctcatcatattccgaattgtcctgactgctgttggtggggagtctatatattatgatgaa
cagagtaaattttcgtgcaacacgcagcagcccggttgcgagaatgtctgctatgacaaatttgcgcccctgtcccacgttcgattctgggttttccaga
ttatcctcatcaccaccccatccattatgtatctggggttcgccatgcatcggattgcacgtgagccagaggagaggcaaaggcgcatggataaaacaaa
gagcaaaaagcgcgctcccataattcatcggggagccatgagagactatgaagaggctgaagataatcaagaggaagaccccatgatttgtgaagaagag
gaaacatgaaaagacagtgacaaaggagacaaaaagaaacacgatggccgccggcgaatcaaacaagacggcttggatgaagtctacgtgctgcaactgc
tggtccgttcagtgtttgaagttggcttcctgatgggccagtatatattataatggggttggaagtcatttccctacctttgggtggcacccgggaaaac
ctgcccttcatacctgtccaactggctttgggggccccggtccctactggagaagaaccctccttttctggctgggatcata

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Library:

Name: NICHD_XGC_Emb4

External Dbs: Unigene laevis cDNA library, Xenopus IMAGE cDNA Library

Description: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.1 kb. Constructed by Life Technologies. Note: this is a Xenopus Gene Collection library.

Anatomy: whole organism	Stage: NF stage 31 to NF stage 32
Normalized: No	Subtraction: No

Vector Details	Vector Map
Name: pCMV-SPORT6 Type: phagemid 5' Restriction Site: NotI 3' Restriction Site: SalI

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Usage:

Whole mount in situ hybridization:	Expressed on Ectoderm and Nerves.
Probes:
Type Size Promoter Linearization Site Description antisense T7 SmaI

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Suppliers:

Search for clone at: EXRC