XB-CLONE-2061754
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Clone Name: alas2-766
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Gene:
alas2.L
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Species: Xenopus laevis
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Description: EXRC Clone Number 766.
Xenopus laevis aminolevulinate, delta-, synthase 2 (alas2), nuclear gene encoding mitochondrial protein, mRNA. To generate probe for in situ.
Clone previously called: IMAGE:6948792
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Sequence:
5' EST: CD326853 
[+]
tccggaattctccggatggacaaggaacatttcagggaccagcaagtatcacgtagacttggagtgtgaactagctgatcttcacaacaaggatgcagcc
ttgcttttctcctcctgctttgtagccaatgactctgctctctttacattggccaagatgctgccaggttgtgagatctactcagatgctgggaaccatg
cctccatgatacagggcatccgaaacagcggcgttaccaaatttgtcttccgtcacaatgaccctgcacatcttgaggaactgcttcaaaaggcagaccc
taaaactcccaagatagtggcctttgagacagtgcattctatggatggtgcaatctgccccctggaggagatgtgtgacgtggcgcacaaatacggtgct
atgacctttgtagatgaggtgcacgccgtgggattgtatggcacgcatggagcaggtgtgggtgagagagatggtgtgatgcacaaaatggatattatct
caggaactctgggtaaggctttcggctgcgtgggtggatacatcgccagcactgcttctcttatagacactgtgcgttcttacgccgccggcttcatctt
caccacttccctccctcccatggtgctggcaggcgcagtggagtctgtgcgggttctgaagagcgaggaggggcaggccctgcgtcgtgctcaccagcgc
aatgtgaaacacatgcgcccactgctgatggatgcagggctgccagtcattaattgcnccagccacatcatcccccatacggttggaaaacgccgccatc
aacaccaggattctgcgacgtcctccttttccaataaaacatccaccgttcaagcccatccaattaccccaactggtggcccccaaggaaaaaaaaactt
tctgaagaagtggggttccctctggccccacacaaaaaaccctggaaataggaaggagccttcctttttttgggaaaagccttttttgtagacccccatg
ggaaaaaaaaggtggggggaatgtccctcttttgccacaa
3' EST: CD324692 [+]ttttnnnnnnnnnnnnnccccccagagaaggggagacgacagggatagggttcttcccaaaacccacatacatcaatgctgcgttactgaagtcccttcc
tttgtctaaagcagagactttcaaagtgcagcaccttggttaatgtctgatacagcactaaagctaaagggcagcaacttcaaggagtggttcaccttta
tgttaacgattagtatgttctaaaatgtcttattctaaacttttcagttagtcttcattttttctttgttttagttttcaaattatttgcctttttcttc
tgattctttccagctttcaaatgggggtcactgtattgttactttttattactcatctttttattcaggcctctcctatccatattccagtctcttattc
aaatcaatgcacggttgctggtctaatttggaccctagcaactagattgctgaaattgcaaactgggagagctgctaaataactcaaaatccaccaataa
taataaatgaaaaccaatgactcagaatatgactctctaccttctactaaaagttaactcaaaggtggaagcatcagtgtttacagtaagtgcaataagc
agaagtttaggtggaagtgaggaaaccgaaaccagaattttgtctgtttcgaatgaatatatggggggcattttatatggcctggaaaaggggatgccag
ggaaatttgcggtttggggaaaaagggaaaaaataaaattcatgggcaaatagtttgggaataaccctttgtggcgtttgaaacatggccaagccaatgc
ccatatggaaaaccgatacccgaaaaaaagaaaagggaatattgggagaaaaagaaacataatgtgggggttttaagagacgccgcacacgaaattgtaa
taatccctttgtgggggggtcgcccccaaccaaggcggggggagattttcctcgtttggagaacacattctcctgctgagtgatgaaccaccccaatttt
ccccgggggtctcaatatataaacgaatttctggggggttatcgaccccccccgcgttttttttttttaacgggtgttctcctttaatccccgcacgccc
ctggggggcaggacagtattgagggggcgggaacaaaacggcacgcctcaataaaactttattctgtacgacaattccctctctcgggagggaagtttct
ctctataaaacaagagaggggcaatatcccactctccgggctcctctcttcgaaacaatgttttttttataatgaacaaaaaatgtgtttcttctggagg
tccttaatcaggggacacactataaaagggaaaacattacgctcagctactttataaaaagcccagtgttatattattaaccaaaataactcacacacag
atatcgacacttcagtgatggccgtgcagaccgcacttcccttatctgcctatcacactacgcnacaacccaaacaactcagcgcgcaga
Library:
Description: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.3 kb. Constructed by Life Technologies. Note: this is a Xenopus Gene Collection library.| Anatomy: eye | Stage: |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
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Whole mount in situ hybridization:
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Expressed on Embryonic haemangioblast, VBI and Myeloid cells.
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Probes:
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| Type |
Size |
Promoter |
Linearization Site |
Description |
| antisense |
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T7 |
SmaI |
EcoRI can also be used. |
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Suppliers:
Search for clone at:
EXRC
