Summary Attributions

Clone Name: gja1-849	Gene: gja1.L	Species: Xenopus laevis
Description: EXRC Clone Number 849. IMAGE:5514939. Gap junction protein, alpha 1, 43kDa. Full Length. To generate probe for in situ

---

Sequence:

Full length sequence: BC073407 [+]

GCAGAAAGCAACAAGCTTTTAAACTTTAAAAAGACAGGTCCAAGTGCCTGTCCTTTAGACTTGCTCCTTTTAGTTTTCAAGAACTGCCTGGAACTTGGGA
TTTTTTTTCCATTTTGGATCTGCAAGGTAACCACTTTTGAGCATGGGTGACTGGAGTGCCTTAGGAAGACTTCTTGACAAAGTTCAAGCCTATTCCACTG
CAGGAGGGAAGGTGTGGCTGTCTGTCCTATTCATATTTCGAATCCTTTTATTAGGGACAGCAGTGGAGTCAGCCTGGGGCGATGAACAATCAGCATTTGT
CTGCAATACTCAGCAACCAGGTTGTGAGAATGTTTGCTATGATAAGTCATTCCCTATATCTCATGTGCGTTTCTGGGTACTTCAGATCATATTTGTCTCA
ACACCGACCCTGCTTTATTTAGCCCACGTTTTTTACTTGATGAGAAAAGAAGAGAAGCTGAACAGGAAAGAAGAAGAACTTAAAATGGTTCAGAACGAAG
GTGGTAATGTAGACATGCACCTTAAACAAATCGAAATAAAGAAATTCAAATATGGCCTTGAAGAGCATGGAAAAGTCAAGATGCGAGGTGGACTGCTTCG
CACCTACATCATCAGCATTTTGTTTAAATCAGTATTTGAAGTTGGCTTCATTATCATTCAATGGTACATGTATGGGTTTAGCTTAAGTGCCATCTATACA
TGCAAAAGAGATCCCTGCCCACATCAAGTGGACTGTTTTCTTTCTCGACCCACAGAGAAAACCATTTTTATCTGGTTTATGTTGATCGTATCTATAGTTT
CTCTTGCCCTAAATATCATCGAGTTATTCTATGTCACCTACAAAAGTATCAAAGATGGCATCAAAGGGAAAAAAGACCCATTTTCTGCAACAAATGATGC
TGTGATTTCTGGAAAGGAATGTGGCTCCCCTAAATATGCCTATTTTAATGGCTGTTCTTCTCCAACAGCTCCCATGTCTCCACCTGGTTACAAGCTTGTT
ACTGGAGAAAGAAATCCTTCTTCCTGCCGCAATTATAACAAGCAAGCAAGTGAACAAAATTGGGCAAATTACAGTGCAGAACAAAACAGGATGGGACAGG
CTGGAAGCACAATTTCTAACACTCATGCACAGCCATTTGATTTTTCTGATGAACATCAGAATACTAAGAAAATGGCACCAGGACATGAAATGCAGCCACT
GACTATATTGGACCAAAGACCATCTAGTAGAGCAAGCAGTCATGCTAGTAGCAGACCACGACCTGATGATTTAGAGATCTAGTCAACGTTGATGAATTAC
CAGAAGGCACATTTTAAGAGTGGAATTGGTACTAAATTCTCTCAAATAAGATTTAACAAACATTTTTGATGTACTTTGATATGGCGAGGGAGAATCGCTT
TGATATTTAAGTAGCTCATACAAATTTTTACAGTCTTAAATGACTCGTACAATATTTCTTTAGAATGTATATCTAAGACAACTTATGTGTTTTCAGTTTG
CCCAAAAAGTGAAATATTTGGGTGAGCAATGTTGTTTTATGACTGAGCAATTAATGTCAGCCTACTATGTATAAGACGAGAGATTTCTTACAGTGATTTT
AGAAATTAACTTAAATAATTGTTTGACTGTTAGGAAACTTTTACAACAATTCTGGAGAAAGACACCTGGATGTTTTCTCAATATGACTAGCATTTTTTAC
TTGCCCCATTTCTTCTTCTGTTTAAGAAAATTGTGCTTATGTAAATAAATTGTTCGCTCACGTGCCTTATTAAAAATGACCTAAAAGCAACATTAAGATT
CCACAATGGGCCTATTGCATCATTTCTAAATTGCCCAAACATTTAATTGTGGATGTCAAATCCCAAATACTACAGTGACTCAAAATGTAAAAATAGTTGA
CCACTTCCATGAGCCTTGAGAGTCTTAGAGTCTTCTTTCCCACCAGACCCAGGATCTTCAGGATCTTGGGACCTCTAGAACATTCCAACAACATCATTAT
TTGTGTGAGACTTTTATTAAGTATTGCAGAACCTTTTATATTAATCTCTAATATTTTCCCTCTGTCTATTTGTGAAATAATTTTAAAGTAAAGGCACCTT
AAATGTGAAAATATCAATTTCATTACTGAAGTAAGACATTAGTGCCTGGTAAACTATTTGGTAACCCAGCTGGTAGAAGCACCCTTTGAATTACTTCCTT
TTTTGCTGGTGCAATATTAAATAATATTTTAAGATATTGTATGTATATGTGTTAAGGCTTGGGGTTGCTTGAAGCTTGGTTGTCTAATGTGGCATGCGAT
GTGGGTCTGACAAAAGAATCATCATTGTGAGTATGCAGCCAGTCAGGAAAAGATGTTAGTGAAAGGCATATGTCTCATAAAATGCCTTATAGTATAGCCA
TGTTCTAGTGGTGTCCTGAGTCAGGAAGCATGATAAAGCCTATAAATCACATGCTTCTGTGGCTATTTTCTGGGAAGATACTAATGTAAATGTGTAGTTC
AAGGAACTGAGGTAAATATTTCTGGAATAACGTTCAGGGAATTTTGCCAGCCACTTATTATATCTTTTATTTTTGTAATAAATTAAGAGCCCAGAATGTT
GTATGCATTTTTATCTAAAGGAAATATGTTTCAAAATGTTGCTGAAGTAACAGTGACGTAATAGTGCATCTGATAAACGAGTTGTATAAAAAGATAATGA
AGAGGTACGTTATATGTCAGAGGTGAATGCTTCTTTAGTAAAACAATGAACCAATATTATGTGTTTACAAATTCCTTATGTGCAGTTTAAGCACAGTTAA
AAATAGTTATTCCTTATCCAAAATCTTTTCCCCCTGTGTCCTATTTTCGATGCAATAACTCAGCTAAGGATATATCTGAACTTTTTATGTGTTTTTTTAT
AGTGTGAGGAAGGCTTTTCTAGCATGAATGGATTAATTTCATTAATGTGTAATCATTGAATGATTGTGCATGTAGTACTGTAAACAGATTTAATGTGGTT
ATTTTTTTTTTAAATATAACTAAAAAATGTTTAGAGCTGGAACATGATTTAAATGTTGCTGCATGGGTCTTGGATTCTGTTGGCCCATTATTGTGAATTA
TGTTGTAATTGTGTACTGTAAACTAGATTTCATTATTCTGAAGAGATTAATTTTGTACATTTAATTGTGGTTTGGTTATTCCAACTCTTAAAATGTTTGC
ACTTGGGATTTTGTTGTTTGGAATGCAAGGGAGTCTGGAATAAGACTTGTTCCATGATATACTTTAGAAGCAATTTATTTTTATAACCACATCTGCTTAA
CACCTCTACCTTTTAGATACCAGGTTCTTGCTTAGGTAGAGCTGAAAAGGCATTTGCCCCAGAGCCCCAAATCAGGGTGGGGTAAAATGCTAAATTAATT
TGTGGTGGCAAAATGCAGATTAAATACAAATTACATAGTATAATGGATAACACTTTTTAGATATTGTCTCTACATAAATCACAGATGTCAATCATTCAGT
GCTTTAGGTGTTGTTAAACTGTGATTCACAGAATATGGAGGGAGTTGTAGCCCAACATTAAAAACAGTTTCTAAATAGTCAACCTTGATTTATGTAATGT
ATAGCCCCTTCAGAGAAGGGTCCTAGTCTTCTTGCATTTCCAGGGCCCATCTTTTTTACCACCAGCCCTAAGTGATACTGCCTTCCTTTGTCTCTATGCA
TTCTATCAAGAAATGGATTGTAGGACTTCTATACAATAGAAGATAATTCATTTTGTACCATACTCATAATTAATGATTTTTTTTTTTTTTTTATAAACTA
CTGTTATGTTCAGCTTCATTGCATGTATGCAGTCATGGTTCATTAGCACATGGTTTCCAGAGTACTTTTCTCTAGACAATAAAGTTTTACTTTTAGTAAA
AAAAAAAAAAAAAAAAAAAAAAAAA

5' EST: CF287677 [+]

cagaaagcaacatccttttaaactttaaaaagacaggtccaagtgcctgtcctttacttttgctccttttagttttcaagaactgcctggaacttgggat
tttttttccattttggatctgcaaggtaaccacttttgagcatgggtgactggagtgccttaggaagacttcttgacaaagttcaagcctattccactgc
aggagggaaggtgtggctgtctgtcctattcatatttcgaatccttttattagggacagcagtggagtcagcctggggcgatgaacaatcagcatttgtc
tgcaatactcagcaaccaggttgtgagaatgtttgctatgataagtcattccctatattttatgtgcgtttctgggtacttcagatcatatttgtctcaa
caccgaccctgctttatttagcccacgttttttacttgatgagaaaagaagagaagctgaacaggaaagaagaagaacttaaaatggttcagaacgaagg
tggtaatgtagacatgcaccttaaacaaatcgaaataaagaaattcaaatatggccttgaagagcatggaaaagtcaagatgcgaggtggactgcttcgc
acctacatcatcagcattttgtttaatcagtatttgaaagtggcttcttatcattcaatggacatgtatgggtttagcttaagtgcatctatacatgcaa
aagagatccctgcccacatcaagtggactgttttctttctcgacccacagagaaaacctttttatctggttaagt

---

Library:

Name: NICHD_XGC_Emb4

External Dbs: Unigene laevis cDNA library, Xenopus IMAGE cDNA Library

Description: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.1 kb. Constructed by Life Technologies. Note: this is a Xenopus Gene Collection library.

Anatomy: whole organism	Stage: NF stage 31 to NF stage 32
Normalized: No	Subtraction: No

Vector Details	Vector Map
Name: pCMV-SPORT6 Type: phagemid 5' Restriction Site: NotI 3' Restriction Site: SalI

---

Usage:

Whole mount in situ hybridization:	Expressed on gills and head.
Probes:
Type Size Promoter Linearization Site Description antisense T7 SmaI EcoRI and SalI can also be used.

---

Suppliers:

Search for clone at: EXRC