XB-CLONE-11928879
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Clone Name: gata2-132
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Gene:
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Species: Xenopus laevis
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Description: EXRC Clone Number 132. Xenopus laevis GATA binding protein 2 (gata2).
To generate mRNA for injection.
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Sequence:
Full length sequence: pBut2xGATA2enMT_FullLength
[+]
tnAACGCTcAnCTTTGGCaGaTCGaCTCTaGAGCTCCTGCGATGGaagTGGcCaCTGACCAGCCCCGtTGGATGGCGCACCACGCTGTGCTCAATGGGCA
GCACCCAGACAGCCACCACCCCGGCCTGGCCCATAACTACATGGAGCCCaCTCAGCTCTTGCCTCCTGATGAAGTCGATGTCTTCTTTAATCACCTGGAC
TCTCAAGGCAACCCCTACTATGCCAACTCGGCCCATGCCAGGGCAAGGGTGTCTTACAGCCAGGCTCACGCTCGCTTGACTGGCAGTCAGATGTGTCGGC
CTCACCTGTTACATAGCCCCGGGCTGCCCTGGCTGGAGAGTGGGAAGACTGCGCTGTCCGCTGCCCATCACCACAACCCCTGGACTGTCAGCCCCTTTGG
CAAGGCCCCACTTCACCCTGCGGCAGCCGGTGGCTCGTTGTACCCGGGCACCGGCTCCTCTGCCTGCCCCTCTTCCTCCCACTCGAGCCCGCACCTGTTT
GGTTTCCCTCCGACCCCTCCCAAGGACGTGTCCCCCGACCCAGGCACCGCGGCCTCCCCGCCCTCCTCTTCCAGGCTTGAGGATAAGGACAGCATTAAGT
ACCAGATGTCGCTCTCTGAAGGAATGAAGATGGAAGGAGGCAGTCCCCTGAGGAGCAGCCTGGCACCAATGGGTACCCAGTGCTCTACCCACCATCCCAT
CCCTACCTACCCCTCCTATGTGCCAGCAGCACACGACTACAGCAGCAGCCTCTTTCATCCTGGCAGCCTCCTGGGAGGCCCGGCCTCCAGCTTCACCCCT
AAACAGAGGAGCAAGAGCAGATCCTGCTCAGAAGGGanagaGTGTGTCAACTGTGGAGCTACAGCCACCCCTCTGTgGanaananaTGGGACCGGCCATT
ATCTgtgTAACGCcTgtgGTCtCTATCacaAAATGAATGGTCAAAACCGGCCACTCATTaAACCCAAGcggaaActGTcggCggcCangaannnGggcac
CTGCTnngCAAACTGTCAgacntcAACTACC
Construction:
Insert:
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| Size: 1360 bp | Source Tissue: | Stage: |
Vector:| Vector Details | Vector Map |
Name: pBUT2
Type: plasmid
5' Restriction Site: XbaI
3' Restriction Site: XhoI |  |
Usage:
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RNA injection:
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Probes:
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| Type |
Size |
Promoter |
Linearization Site |
Description |
| sense |
2500 |
T3 |
EcoRI |
Gata2 was PCRed out of psp64T and is missing an EcoRI site at 1318bp of the Gata 2 cDNA. It is therefore possible to linearize with EcoRI |
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Suppliers:
Search for clone at:
EXRC
