XB-CLONE-4377016
Clone Name: IMAGE:6999896
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Gene:
cyp27b1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC075285 [+]
TGCCAAGTGTGAAGGATGCTACTCGCAAAGGTGCCCCCTGCCTCTAGCGCTCCCCTCAGGGCAACATGGCACAGACCCTCAAACTGGGCAGCAGCAGGTC
CTCCCAGCTCTTCAGAGGGCTCCAAGAGCTCTGGGCTGAGACGGTCCTCAAGAACTCAGAGAAAGTCATCAAAGGGCACAAGTCATTGGCTGATATGCCC
GGGCCCAGCACAGTCAGCTTCATTTCTGATCTGTTCTGCAGGAGGGGACTGGCACGGCTACATGAGCTGCAGCTGGAAGGGAAAGCCAAATTTGGCCCAG
TCTGGAAGGCAAGCTTTGGACCCATTCTGACTGTTCACGTGGCAGAACCATCACTCATTGAGCAGGTCCTTAGACAAGAAGGAAAGCACCCTATTCGTTC
AGATCTCTCCTCATGGAAAGATTACAGGCAATGCAGGGGACACTCATATGGACTCCTTACAGCGGAAGGTGAAGAATGGCAGCAGTTTCGCAGTATTTTG
GGAAAACATATGCTAAAACCCAAGGAGGTGGAAGCTTACAGCGATGTTTTAAATGATGTTGTTGGAGACCTCATTAAAAAAATCAATTATCAGCGGAGCC
AAAACCAAAACAACGTTGTCAAAGATATAGCCAAGGAGTTCTACATGTTTGGATTAGAGGGAATTTCCTCAGTGCTTTTTGAGTCCCGCATCGGGTGCTT
GGAACCCACTGTGCCCAAAGAAACAGAGAAGTTCATTCAGTCAATCAACACCATGTTCGTGATGACTCTTCTTACCATGGCCATGCCCAAATTTCTACAC
AAAATTTTCCGAAAGCCCTGGCAAAAATTCTGTGAATCCTGGGATTACATGTTTGCATTTGCCAAAGGACATATCGACAAAAGAATGAAGGACGTTGCAC
AGAAATTAGCACAGGGTGAAAAAGTTGAGGGAAAGTACCTCACCTATTATTTGGCACAGGAAAAAATTCCCATGAAGTCAATCTACGGCAATGTCACAGA
ACTTCTCTTGGCTGGAGTGGACACGATTTCAAGTACTCTTTCCTGGAGTCTTTATGAGCTAGCCCAGCACCCAGACATACAGTCTGCTGTGTACAGTGAG
GTCGAGGAGATACTGCAAGGCAAACAAATCCCATCGCCGTCAGATGTGGCCAGAATGCCTTTACTGAAAGCTGTTGTAAAAGAAGTCCTACGATTGTACC
CTGTTATTCCTGGCAATGCTCGTGTAGTCGCTGACAGGGATATTCAAGTGGGAGACTATATCATTCCAAAGAAGACATTGATCACCCTCTGCCACTATGC
CACCTCCAGAGATGAGAACGTCTTCTCAAATCCCAATGAATTTCAGCCCGATCGTTGGCTAAAGAAAGAAGATACACATCACCCATATGCCTCTCTCCCA
TTCGGATTTGGCAAGAGGAGCTGCATTGGAAGACGAATAGCTGAACTAGAGGTTTATTTGGCTCTGGCAAGGATCCTTTCCCACTTTGAAGTAAAACCTG
AGCAGCCAGGCAGCCTTGTCATGCCTATGACTAGAACTCTGTTGGTGCCAGAAAAAGAAATCAACTTGCAGTTTCTGGAACGATGACATGAGCCGCTGCT
TAGTGTGGATTAAAAAGGCATCCGGTCACAACAATTACTGTGAAATATTGAAGTCTCCACATAGGGAATTCCCACATTTTTTGATACAAAGAGCTGTGAT
CACAAGAGCGTGATATCCAGTTACTAGTCCCTGGTGGGTTTCCTTACTCAGCATTCTCTGTATCTCTCCTATGCTGTATTCCACCAGAATGTGCAATAGC
CATGGTGCCCATCTGCCATGCCTTTGTTCCATAAATATTTATACCGCTATGGGACCTGTTAGCTAGAATACTAGGGACCTGGAGTTTTACGGATAAAGGG
TCTTTATAAAATTTGGGTCCCCATACGTTGTCTATTAAAAATCATTTAAACATTAATTAAACCAAATATGATTGTTCTGCCTCAAATGAGGATTAATTAT
ACCTTAGTTGGCATCAAGTACAAGATACTGCTTTATTATTACAGAGAAAAAGCAAATCATTTTTACAAATGGAGTCTATGGGGGATAGCCTTCCCATACT
TCAGGACTTTCTGGATAATGGGTTTCCAGATAACTGATCCCATATCAGTATCATGAAAACAGCAAAACCCCATAATACATAAGTATTACAGAAGTAATAT
TGTACACACCAGTGTGGCTTATAATCAACTGTCACAAGCAAGCTCTTTGCTGATGGCATGTGTTTAATGTATATAAAAGGAGATACTAATGGGAGAGACA
TGAAACAAATTTCTAAAATGTCCATAATTGTCTTATTATGTGGTGTAATAATACCTTTTGTATTTGTGAGTCACCACCATCTAACTGTATGTCCATTAGA
AAGCAGCAAGACTTACATAGATCCCAATATGTGACCTGACGTGGTTACTTCCTGTTACCTTTCAACATTTTATTTTATTAATGACAAACTATTTGTACTT
ATGTAAAGCTTATTTATTTATATGTATATATTGGCCTTTACCAAGAGTTTTATATTTCAATCTCCATATTTAAGCCTAAATACTATGTATTCAAAATATT
TTGTATTTATACATTGGGGAAACATACTTTGAGGTTATGTAAAAACATTGTGAACCAAATAACTTTTTTTGTATTTAATATATATATATATTTATTATTT
TGTGCTAATAAATATGTAATTTTATACATGTGAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CF343253 [+]atatatatattaaatacaaaaaaagttatttgcttcaaatgtttttacataacctcaaagtatgtttccccaatgtataaatacaaaatattttgaatac
atagtatttaggcttaaatatggagattgaaatataaaactcttggtaaaggccaatatatacatataaataaataagctttacataagtacaaatagtt
tgtcattaataaaataaaatgttgaaaggtaacaggaagtaaccacgtcaggtcacatattgggatctatgtaagtcttgctgctttctaatggacatac
agttagatggtggtgactcacaaatacaaaaggtattattacaccacataataagacaattatggacattttagaaatttgtttcatgtctctcccatta
gtatctccttttatatacattaaacacatgccatcagcaaagagcttgcttgtgacagttgattataagccacactggtgtgtacaatattacttctgta
atacttatgtattatggggttttgctgttttcatgatactgatatgggatcagttatctggaaacccattatccagaaagtcctgaagtatgggaaggct
atcccccatagactccatttgtaaaaatgatttgctttttctctgtaataataaagcagtatcttgtacttgatgccaactaaggtataattaatcctca
tttgaggcagaacaatcatatttggtttaataatgtttaatgatttttatagacacgtatggngaccaan
5' EST: CF343252 [+]cggattccccgggattgccaagtgtgaaggatgctactcgcaaaggtgccccctgcctctagcgctcccctcagggcaacatggcacagaccctcaaact
gggcagcagcaggtcctcccagctcttcagagggctccaagagctctgggctgagacggtcctcaagaactcagagaaagtcatcaaagggcacaagtca
ttggctgatatgcccgggcccagcacagtcagcttcatttctgatctgttctgcaggaggggactggcacggctacatgagctgcagctggaagggaaag
ccaaatttggcccagtctggaaggcaagctttggacccattctgactgttcacgtggcagaaccatcactcattgagcaggtccttagacaagaaggaaa
gcaccctattcgttcagatctctcctcatggaaagattacaggcaatgcaggggacactcatatggactccttacagcggaaggtgaagaatggcagcag
tttcgcagtattttgggaaaacatatgctaaaacccaaggaggtggaagcttacagcgatgttttaaatgatgttgttggagacctcattaaaaaaatca
attatcagcggagccaaaaccaaaacaacgttgtcaaagatatagccaaggagttctacatgtttggattagagggaatttcctcagtgctttttgagtc
ccgcatcgggtgcttggacccactgtgcccaaagaacagagaagtcatcagtcatcacacatgtcgtgatgacctt
Library:
Description: Bulk tissue was collected from a whole 10 month old male from the F6 strain. 1st strand cDNA was primed with a Not I - oligo(dT) primer, double-stranded cDNA was cloned into the Not I and EcoRV sites of pExpress-1. Library was size-selected for >1.5 kb fragments for an average insert size of 1.92 kb. A normalized version of this library is also available (NICHD_XGC_Swb1N). Library was constructed by Open Biosystems (Huntsville, AL). Note: this is a Xenopus Gene Collection library.Vector Details | Vector Map |
Name: pExpress-1
Type: plasmid
5' Restriction Site: EcoRV
3' Restriction Site: NotI | |
Usage:
Suppliers: