XB-CLONE-4370727
Clone Name: IMAGE:7006830
|
Gene:
smad9
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC075389 [+]
TCCGCATGTACTGAGGCCGGAGGGACCCGCACAGCAGAGACACAGGACATATGGCCAAATCCTTGAGAGATGTATAAGTTTTTCCCAGAGGTGTTTTTAC
ATTGAAGAACGAGTGAAAGTTTTCTTGCTGCCTGTACTCACTGCTTCTTTAGACTTCATCCTGGTTTGATCTGAAGGTAAACTCAGCTAATCCTATGCAC
GCCAGCACTCCCATCAGCTCTTTGTTCTCCTTCACTAGCCCTGCTGTCAAACGGCTGCTTGGCTGGAAGCAAGGAGACGAAGAAGAAAAATGGGCAGAGA
AAGCGGTGGACTCGCTTGTGAAGAAACTGAAGAAGAAGAAAGGGGCAATGGAAGAACTGGAAAGGGCTTTAAGTTGTCCGGGGCAGCCTAGTAAATGTGT
CACCATCCCGCGTTCACTGGATGGGAGGTTACAGGTGTCCCATCGCAAAGGTCTCCCCCATGTCATCTATTGCCGGGTTTGGAGGTGGCCTGATCTGCAA
TCCCATCATGAGCTGAAACCCATGGAATGCTGTGAGTTCCCTTTTGGCTCCAAGCAGAAGGATGTGTGCATAAATCCCTACCATTACCGTAGGGTGGAAA
CACCAGTGTTACCACCAGTGCTTGTTCCAAGACACAGCGAGTTCAACCCACAGCTAAGCCTTCTAGCAAAGTTCCGAAACACCTCGCTTAATAATGAACC
ACTTATGCCGCACAATGCAACTTTCCCAGAGTCTTTCCAGCAGCAGCCCCCATGCACTCCATTCTCTTCCTCACCGAGCAACATCTTCCCTCAGTCCCCA
ACCACGGTGGGCTATCCAGATTCCCCTCAGAGTTCCGCTGACCCAGGAAGCCCATACCAGATCACAGAGACACCCCCTCCGCCATATAATGCTCCAGACA
TTCAAGGAAATCAAAACAGACCCACTGCAGACCCAGCTGCATGCCAGTTAGTTCTGTCGGCACTAAACAGAGACTTTCGCCCAGTTTGCTACGAAGAGCC
TCTGCATTGGTGTTCTGTTGCTTATTATGAACTGAATAACCGAGTAGGGGAGACCTTCCAGGCCTCCTCACGCAGCGTCCTCATTGATGGATTCACGGAC
CCCTCTAATAATAAAAACAGATTCTGCTTAGGGCTTCTGTCAAATGTCAACCGCAACTCCACTATTGAGAATACCCGCAGGCACATTGGGAAAGGGGTTC
ATCTTTACTACGTGGGAGGCGAGGTGTATGCAGAATGTGTGAGTGACAGCAGCATTTTCGTACAGAGCCGCAACTGCAATTACCAGCATGGCTTCCATCC
TTCCACCGTCTGCAAGATCCCCAGTGGCTGCAGTCTGAAGATATTTAATAACCAGCTCTTTGCCCAACTTCTTTCCCAGTCAGTTAACCAAGGCTTTGAG
GTTGTTTATGAACTGACGAAGATGTGCACCATTCGAATGAGCTTTGTCAAAGGATGGGGAGCCGAGTACCACCGACAGGATGTAACTAGCACCCCCTGCT
GGATTGAGATCCACTTACACGGGCCTCTGCAGTGGCTGGACAAGGTTCTGACACAGATGGGCTCACCGCATAATCCAATCTCTTCTGTATCGTAAACTCT
CTGCAGCCACATATGGCAAGCAATGGCATACTGTACTAGCTTGCCCTTATATAAAAGAGCACATAATGCTAGCCACACATATCTGCACAAAAGGCTCCAT
AACCCATGACTTATCATAATCCTTTTAGCTCTGACTTTCAGGTGTTCTGCGTCCCCCGCCCCATATATATATGGGGCTTCACTTCACACTCTCATTCCAG
GAAACAGAGCCACCATCGAAGGGGACAGGTGGGAGGCCAGTCAGAGTCCCCCCATCACAGTGGAGAGCCTGTACATACATGGTGGTCCTGAATTATGGCT
CCCAAAAAGAATAGGACTTTATAACATGGAATGAGGTTTGGCCTAGAGACCTGAAGCAGGTTGGGGACCGGCTGAATACCCACAAAATGGTTGGGTTTTG
GGTGGCGTTTGGCTTTTAAAGGAATGCATTTCTTTTATCTTTACCTGTCTGATCCACCTCCAGAGTGATGCGGATCCTGGGTTCAGGTAGTGGGCCAGGG
TAGGGCCCATAGTGGGGTGGCGGGTCGGGTTGCGGGTTTGGACCAGGAGTGGGTGTTTGGTAAAATAGGGCATGTCCTGGGTAGGTCTGGGCTACCATTC
TACTCAGTGGGCATTACACAAGGTGGCCCAGCTGGGCAAAGGGCTAAGAACCCAGTAGTATGGGTGCCCATGATTTCTGATGGTGGCCCTGCCAGGAAAA
AGTAGATTCTAAATACACTCCTTCAAGTTCCCTACTCCTTGCTTTAAAAACCTTACAAGTAATAACCTTATATCAGGTTACTTGGGGGATAATGATTGAG
ACCTGGGCCCCCCATTAGCATTTGCTTTATTTACACCCATGGGTTAGTGCTACAGAGCCGGCTGATATGCAGGCAAAGCCCATGTATGGCTGCATGAACA
ATGTGCCCTTTTATAGAAGAGCCAATCATCTTCAGGCCTTACTGTTGCCATTTTTGTCAGCAGGTAACTATTTGCCTTGTAAATATATGCATTTCACTAG
TACTGTGTTTTTAGTGTCATGGTTAGATGTTCATGTGTTTTTTATATATAAATATAATGTACATACTGTTTCTATGGAGAGAGCACTTCACTAGTACTGA
CTAGAAGAATAAGGGCGGAACGGAGTGTTACTTTCTTTCTGTCGGCTTCTCAGATGGAGAAGCAAAGTAGTCCGGGTCCATGCACTTAAATACGGTACGT
TTTGGACAATTTATGTGTTTTTTGTTTTTTAAAGAATTATTTAATTATTTTTAGATTTCTCTTTTTCCTTCCTGGTTCCCATATTGCTCTGTACTGCATT
GTAAGGGGGAAGATTTTTATATTTGTTTTTTTGTATAGTGAGTTAGAGATAAAGACTGTCGTTCTGACATATATAGGGATCTAGCAGCCTCTGCCACTGA
CCCTGCCAGCTCTTTTATTCAGGAATGCACCCCGCCCCCTTAGTTGTAATGGGCCTCTTACTTAATCCAGAATATTAAAAAGAACTAGATCTCTGGATAC
CTGGATATAGAACAGAAAGTACCGGCATGTCCTCTGCCACTGATCTGGAATGGACAGTTGGTTCTGATTGGGTCTCACATGCTATTCCATACAGCATTGT
GAGCGTATATCACTATATATGTAGTGTTGCTGGCCAGGTAGTCACTGTATCTCATCCTGTGCTTCTGGTGTGCTGGCTCTCGGGGATATACTGTAATATC
TGAGATATACTCTGTCCGATGTCAGATGTATCAATCAGTAAATAAAGAAGTAAGATACCAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CF376127 [+]tccgcatgtactgaggccggagggacccgcacagcagagacacaggacatatggccaaatccttgagagatgtataagtttttcccagaggtgtttttac
attgaagaacgagtgaaagttttcttgctgcctgtactcactgcttctttagacttcatcctggtttgatctgaaggtaaactcagctaatcctatgcac
gccagcactcccatcagctctttgttctccttcactagccctgctgtcaaacggctgcttggctggaagcaaggagacgaagaagaaaaatgggcagaga
aagcggtggactcgcttgtgaagaaactgaagaagaagaaaggggcaatggaagaactggaaagggctttaagttgtccggggcagcctagtaaatgtgt
caccatcccgcgttcactggatgggaggttacaggtgtcccatcgcaaaggtctcccccatgtcatctattgccgggtttggaggtggcctgatctgcaa
tcccatcatgagctgaaacccatggaatgctgtgagttcccttttggctccaagcagaaggatgtgtgcataaatccctaccattaccgtagggtggaaa
caccagtgttaccaccagtgcttgttccaagacacagcgagttcaacccacagctaagccttctagcaaagttccgaaacacctcgcttaataatgaacc
acttatgccgcacaatgcaactttcccagagtctttccagcagcagcccccatgcactccattctcttcctcaccgagcaacatcttccctcagtcccca
accaccgtgggc
3' EST: CF376126 [+]gatatctcagatattacagtatatccccgagagccagcacaccagaagcacaggatgagatacagtgactacctggccagcaacactacatatatagtga
tatacgctcacaatgctgtatggaatagcatgtgagacccaatcagaaccaactgtccattccagatcagtggcagaggacatgccggtactttctgttc
tatatccaggtatccagagatctagttctttttaatattctggattaagtaagaggcccattacaactaagggggcggggtgcattcctgaataaaagag
ctggcagggtcagtggcagaggctgctagatccctatatatgtcagaacgacagtctttatctctaactcactatacaaaaaaacaaatataaaaatctt
cccccttacaatgcagtacagagcaatatgggaaccaggaaggaaaaagagaaatctaaaaataattaaataattctttaaaaaacaaaaaacacataaa
ttgtccaaaacgtaccgtatttaagtgcatggacccggactactttgcttctccatctgagaagccgacagaaagaaagtaacactccgttccgccctta
ttcttctagtcagtactagtgaagtgctctctccatagaaacagtatgtacattatatttatatataaaaaacacatgaacatctaaccatgacactaaa
aacacagtactagtgaaatgcatatatttacaaggcaaatagttacctgctgacaaaaatggcaacagtaagggctggagatgattggctcttctataaa
aggcacattgttcatgcagccatacatggggctttgcctgcatc
Library:
Description: Bulk tissue was collected from a whole 10 month old male from the F6 strain. 1st strand cDNA was primed with a Not I - oligo(dT) primer, double-stranded cDNA was cloned into the Not I and EcoRV sites of pExpress-1. Library was size-selected for >1.5 kb fragments for an average insert size of 1.92 kb. Library was normalized to Cot5 with a 180-fold reduction of actin. A non-normalized version of this library is also available (NICHD_XGC_Swb1). Library was constructed by Open Biosystems (Huntsville, AL). Note: this is a Xenopus Gene Collection library.Vector Details | Vector Map |
Name: pExpress-1
Type: plasmid
5' Restriction Site: EcoRV
3' Restriction Site: NotI | |
Usage:
Suppliers: