XB-CLONE-4363472
Clone Name: IMAGE:7021694
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Gene:
farsb
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC089642 [+]
GTAGTGATTACAGAACCATGCCGACTGTCAGTGTAAAGAGGGATCTGCTGTACCACGCTTTGGGCCGCACTTACACTGATGAAGAATTTGATGAACTCTG
CTTTGAATTTGGCCTAGAGTTGGATGAAATTACCTCAGAACGTGAGATTATCAGCAAGGAGAAGGGTGATGTAAAGGCAGAAGGTGCCTCCGATGTTATT
CTATTTAAGATTGATGTACCTGCCAACCGCTATGACCTTCTGTGCATAGAGGGGCTGGTGCGGGGGCTACAGGTCTTCAAAGAAAGGTTAGAAGCACCAA
GGTACAAGAGGATCACTCCATCAGATCAAGGGCTGCAGAGACTTTTCATCACAGAGGAGACTGCTGTAGTTCGCCCCCATGCAGTGGCTGCAGTACTCCG
GAACATTACATTTACTCAGGAACGATATGATAGTTTCATTGATCTGCAGGAGAAGCTTCACCAGAACATCTGCAGGCGGAGGACTTTGGTGGCAATTGGC
ACACATGACCTGGACACTATCTCTGGCCCATTTGTATACACAGCTAAACCACCTGCAAGTATTAAATTTAAGGCCTTGAACCAAAGCAAGGAGTTAACAG
CCCCAGAGCTAATGGACCTGTACAGAACTGACAGCCATATGAAGCACTATCTGCACATTGTTGAGGATAAGCCTGTTTATCCAGTAATCTATGACCGCAA
CGGAGTGCTGCTGTCCATGCCGCCGATCATCAATGGGGATCATTCAAGAATCACCTTAAATACTCGCAACGTATTCATTGAATGCACCGCAACTGATTTG
ACAAAGGCCAAAATAGTCTTGGATATTATGGTCACCATGTTTAGTGAATACTGTGCTGAACCATTCACTGTAGAGGCAGCAGAAGTTATTCAGCCAGATG
GATCGACTTCTATTTACCCAGAACTGGCGTACAGGAAGGAAGTGGTACCTGCTGAGAAGATGAACTCTAAAATTGGAATCAGTGAAACACCCTCCAACCT
GGCAAAGCTTCTTACACGCATGTGCCTAAAGTCTCACGTGCTGGAAGATGGGGAGCAGATAGAAATAGAGGTGCCCCCAACAAGAGCAGACATTATCCAA
GCCTGTGACATTGTGGAGGATGCTGCTGTTGCTTATGGGTTCAATAACATCAAGATGGTTATTCCTAAAACTTACACAATAGCCAACCAGTTTCCACTCA
ATAAACTCACTGAGTTACTGCGCCAGGACCTGGCTGCTGCTGGATTTACAGAAGCCCTGACATTCGCTCTGTGCTCTGTAGATGATATCTCTGATAAGCT
CGGGATACCAGTCTCTGCCACTAAAGCTGCACATATCTCCAACCCTAAAACAGCTGAATTTCAGGTGGCTCGCACAACTCTTCTCCCAGGCCTGTTGAAA
ACCATTGCAGCCAACCGTAAAATGCCCTTGCCTCTGAAGCTGTTTGAGATCTCTGATGTGGTTGTAAAGGATCCCACTAAAGATGTGGGTGCCAGAAATT
ATCGACATCTGTGTGCAGTTTACTACAACAAGTACCCTGGATTTGAGGTGATACATGGCCTCCTGGACCGAACCATGCAACTGCTGGCACTGTGCCCGGG
GGAGACAGGGTACCAGATCAAAGCAGCACAAGATCCAGCCTTTTTTCCTGGTCGCTGTGCCGAGATCTTGGTTAATGAGCAGATTATAGGCAAACTGGGT
GTCCTGCATCCTGATGTCGTCACTAAATTCGACCTGACCTTACCGTGCTCTGCGCTTGAGATCAACGTGGAGCCTTTCCTGTGATGTCTCTTTTGTACTA
CCCTGCTTTATCTGCACAACCACTCCTCTGGCAAGATACTTTTTAAATAGAGCAGCCTCATGTAATTTATTAGGAGCAACATATTTCCAGGCATCATTCT
AAATACTATTGACATGATGTGAGCTTACTGAGTTCTTGACAGTAATCTGTTTGGTTTAATAATGCCACTTTGGCCCTTGATTGACTGAAGTGTAAGAGCC
AGTACATTTGCCTGTGATCAAACAGTAGTTACCTACTCAGCACCACATCACAGGCACAACTTTGGCTATAGCTTAGCTTGGTTATGATAAAATTGAGAAC
CGTTTATTGTAACTGAGCTTCTCTTTGTGACTTCAATGTGACTACTCACAGCAGTCAAATCATTGTCAAAAGGCTTTGATAAATATTCCAATTAGTTAAA
CTTTCCTGACTCCGTCTGGATTCCTCAGTTTAAAGTTCCAAACTATAATGCCCCTTATATATAAGTTATATCCTTTGTGGTCACTTCCACCAGAATAAGA
AAGTCAACATGGGTCCCAGCTGTATTGTAGAATCAATATCCCTAGCTGCCCTGAGGCACTGCTGCTTATTGGCTGAGGGAACAATACTCTCTTTCACAGC
TTTAGTTTAGTTCTCACCTAAATGCAGTCATATTAATCTTTAGAGAAGTCATTATAAGAGGTCCACCTGTGCTAATGATAAAAACAAGCACCCCTGGTGG
TCCTTGAAGCCTGGGATTTGAGCCATTGATCTTTTGCTTTACAGAAGAAGCAGCTGAAAGTGGTGGTTATAGGTTGCTCAACTGTTCAGACAACTATAAA
CCCATTAATGGGAAAGTTAGAAGGCATTTATCTCAGTGGGAAACCGCAGATGAGGTGCTTTGTTATGGCACTGATTACCAGGAAGATTTTGCATGTAGGC
TGTACCATCTGTACCTGTTTCAGAACAAGACTGCACATCAGACTATTATTTACAAGGATTTACAGACAGTCAAGGTTTTTGGTGACTGATTTATGATGGA
CTTGGCTGTTCTTAATGACAGTCAGAAACAATATCTGGCAAATGATGGCACTGGAGCTATGTGCTCTTGGCCTTATCCTGATTGCTGTAGCCATCAAGAG
GCCTGGTCAGTCTATGCTGTTGCACATATTAATAAAAGACTATGGCCTGGGCCTTTTTTCTATTTAAAAATGTACCTTGTCTAAAACGCAAATGCTATAC
CAGTGAAATCACTTGAAAATGATGCTCTTCAGTCACATCATATTTTTTTTTTTAACTGCATAACCACAACATTTAGTGTGAATGAGGTCCAGGATGTTGC
AAACCTGAAATCTTATACTTCTGTATTTATTCTTACATCATGAGGTGCAAAATAAACAAAGTTGTTTAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CF589372 [+]agtttcggtaaggattcccgggatgtagtgattacagaaccatgccgactgtcagtgtaaagagggatctgctgtaccacgctttgggccgcacttacac
tgatgaagaatttgatgaactctgctttgaatttggcctagagttggatgaaattacctcagaacgtgagattatcagcaaggagaagggtgatgtaaag
gcagaaggtgcctccgatgttattctatttaagattgatgtacctgccaaccgctatgaccttctgtgcatagaggggctggtgcgggggctacaggtct
tcaaagaaaggttagaagcaccaaggtacaagaggatcactccatcagatcaagggctgcagagacttttcatcacagaggagactgctgtagttcgccc
ccatgcagtggctgcagtactccggaacattacatttactcaggaacgatatgatagtttcattgatctgcaggagaagcttcaccagaacatctgcagg
cggaggactttggtggcaattggcacacatgacctggacactatctctggcccatttgtatacacagctaaaccacctgcaagtattaaatttaaggcct
tgaaccaaagcaaggagttaacagccccagagctaatggacctgtacagaactgacagccatatgaagcactatctgcacattgttgaggataagcctgt
ttatccagtaatctatgaccgcaacggagtgctgctgtccatgccgccgatcatcaatggggatcattccagaatcacccttaatactcgcaacgtattc
tttgaatgcaccgcaactgatttgtacaaagcccaaataatccttggaaattatggtgcacccatgtttagtggaatacggtgctgaaacctttccccgg
taaaagccccccaaattttttttcagcccgaaagggaatcgaccttctattttacccccaaaactggcccttacaaggaaaggaaatggggtcccctcgc
ctgaaaaaaaataaaaat
Library:
Description: Bulk tissue was collected from a whole 10 month old male from the F6 strain. 1st strand cDNA was primed with a Not I - oligo(dT) primer, double-stranded cDNA was cloned into the Not I and EcoRV sites of pExpress-1. Library was size-selected for >1.5 kb fragments for an average insert size of 1.92 kb. Library was normalized to Cot5 with a 180-fold reduction of actin. A non-normalized version of this library is also available (NICHD_XGC_Swb1). Library was constructed by Open Biosystems (Huntsville, AL). Note: this is a Xenopus Gene Collection library.Vector Details | Vector Map |
Name: pExpress-1
Type: plasmid
5' Restriction Site: EcoRV
3' Restriction Site: NotI | |
Usage:
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