XB-CLONE-4361834
Sequence:
Full length sequence: BC094950 [+]
CAGTGTGGTCTGCGTGAATCGAGATGGATTCTGCATTACAGACTACAATTATATTACTGAGCATCTGCTCCCTTCATGTTGCTGCCTATCCCAATGGGAA
AGTAGAGGTCGCCTGCGGTACGATGGAGCCAAACCATGGAGCTTCACCCCAAACATCTGCAGCTCCTTATTCTCTGGTTGTGTCTAATACTACCTACGGG
AATGGACAAAGCATAACAGTCACACTCAATAACACATCGCAGGGAATTCCTTTTGAAGGCTTCTTGATACAGGCTCGTGCCGTTGGGGGTCACAAACCCC
TGGGAACCTTCCAAGTCAGCGATTCAGCTGCCCAGACTCTGACTTGCAACACTGCAAATAGTGCAGTGAGCCACACCTCTGATGCTAATAAAACACATAT
TCAGGTCACATGGCTGGGACCAAAGACCAAGGAAACCATTGAGATCAGAGCCACAGTTGTACAGAGTGCAAAGGTTTATTGGGTCTCTGTCCAAAGTCCA
AAACTTTCATATTCTGGAGGAGTCAGGCTTTTGGCCCCTGCACTCAGCCAGCTACTCCTCGGTACCTCTGTACTGCTGTTGGCGCTGCTCACTGTCTGAT
GGCTACGGTCTGACACAAACTGGAAACAATGCCAAGATGATTGTCACACTTTATCAATAGGTTGTGTGTCCTTACAGAGAAACATTGTCAGGCATATAGC
AGTGATACCCAGGGAAAAGGCAGTTGTTACTGTTGAATTGCATAAATGTATATAAAATGCATACCTCGCACACCCTCTAAAGGGCTGTCTATGTAAAAGC
CTGAAGTAGAATTTACACCAGTGTTTCTTACTTTTTTCTCAGGAAACCCAAATTAAGTTGCAGAATCAGATTCGTGGCCCAAAACCTGGTGTAGAACCTC
TTATTCACGTTATTCTTATTTAGGAGGATAATGGAGACCTTCCCTAAACAGCGAGGGGATCCATTTCAGTGATATCATTACTCATGTTTTGCTGGACTGG
TTCTGTCGGCCTATGTGCACAATACAACACATTAACATGAAACTACACTTTCCAACAATGCTGGATCAGGTGGGGTTTCATTTTAATGATGTACATTTTT
ATTGTAATGGCAGAGTCAGTGTAATAAATGTGACAGTACTGTATGTTTATGTGTTACTAAACAGTTGCTACTGTATGTAAAGCTAGTAATGCTGTGTGAT
ATTTCCACAAATAACCATTTGATTTTACAGTGATATTTGAGTGCAGACAGTCACTAAATCCTTAGAGTTTTACCATTCAAATGTTATTTCCAAGGGAGCT
GGGACCAGGGAATATTAGCATTTGGCCTCCTTCCCTAATTTTCCAACAGACATTCACATGCACCTTGATATAACAGAGCTGAATGTGATTTTTTAACAAC
ATAATTGGAACTCTGAAGAGATAATTTTCAACATCAAAATCAAATCCAAATCAAATTTAAGTGAAAATCTATTTGCTTGTAATTTTTTGTTTCAGCAGAG
ATGAATGGGAATTCTATTTCATGTGGAAAGCTCCTGACTGCTGAATCTGCTGTGTTTCCTTAGAATTACAGGTATAGGACCCTGGGGTTTTCTGGATGGC
TGATCTTGCCGTGGTTTGGATCTCTGTACCCTAAGGGGCACATTTACTAATCCACGAATCCGAATCACGAATGGGAAAAAATCGCATTGGAAACAAAAAT
TTTGCAAGATCGCAAATATCACGAAAATGCTTACGAAAAAATCGTATTAGTCACGATAATATTGTATTGGCGATCCGAAAGTCACGAAATTTTCGTACCG
AACAATTGTAAACAGTGGTAAAACCTTTCCGATTTTTTGTGCAAACGTCCGAAAAAGTCGTGCGGCGTACGAAAAAGTCGTGCGGACGCCCGAAAAAATA
GTCGAAAATACGCTCGGAGCGTTCGTGCTTTTGTAAATGTGCCCCCAAGTCTACTAAAAATCCTTTTAATATTGATTTAAACTAATAGGATTGTTTATCT
TACAATAGGGATTGATTGTATCGTGGTTGGGATCAAATGCAAGGTACTGTTTTATTATTACCTGGAAACAGGAAATCATTTTTAAAAATGTGAATTATAT
AATTAAAATATAGGAGATGGCCTTCCCGCAATTTGGACATTATGGGTTTACAGATAGCAGGTCCCATACCTGTTTACTATTATTACAGGTCTGAGCAAAA
CACTAAACTACCAAGAAACATCTGTGTAAGATTTTATGCCTTATCCGGAAAACCGTTATCCAGAAAGCTCCGAATTACGGAAAGCCTGTCTCCCATAGAC
TCCATTTTAATCAAATAATACATTTTAAAATATATTTTTTATTTTTTATTAATAATTAATAATAAATTTATTTTATCATTTTATTCTTTTTCTCTGTAAT
AATAAAACAGTACCTTGTAACTGATCCCAATTAGGATATAAATAATCCTTGATGGATGCAAAACAATCCTATTGGGTTTAATTAAGTTTTATTGATTTTT
TTAGTAGACTTAAGGTGTGGAGATCCAAATTGCGGAAAGACCCCTTATCTGGAATACCCTTGGTCCCGAGCATTCTGGATAATTCTGGATAAAAAACGTC
TACAGGGGAATTTAAAACCAGTGTTTTCAGGAATAGTGGCTTTTATTGGTAAAAGAAAAAAATGTTAAGGTAACCTGATTCTTAATATGGGTTAAACAAA
TCCAGTCTTACAGCTTTTCATGGAAAGCGCCTGGGAAATGTATAATCCCTTATTTTCACCCCACAACAGAGCTTAGTTCCAATAACAATGATCTGGGAAT
TATGTTACAGCATCAGCCTCAGGTGAGGGAAGAAGTACAGAATGAATTTCTGAATACTTATAGAAGATCCTTATTATTTCCAGTAACTCTAGGCTGCAAG
CTCTCAAGCGGGGCCTCAGACTTGGCTGTGTTTGTATATTGTATTTATACTGTAACAAGCTTAATTACCCTTGTTCCCTATTGCACAACACTGCAACTTT
GTAGAACCTTTATAATAAAAATATTTTCATGAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CF590699 [+]cagtgtggtctgcgtgaatcgagatggattctgcattacagactacaattatattactgagcatctgctcccttcatgttgctgcctatcccaatgggaa
agtagaggtcgcctgcggtacgatggagccaaaccatggagcttcaccccaaacatctgcagctccttattctctggttgtgtctaatactacctacggg
aatggacaaagcataacagtcacactcaataacacatcgcagggaattccttttgaaggcttcttgatacaggctcgtgccgttgggggtcacaaacccc
tgggaaccttccaagtcagcgattcagctgcccagactctgacttgcaacactgcaaatagtgcagtgagccacacctctgatgctaataaaacacatat
tcaggtcacatggctgggaccaaagaccaaggaaaccattgagatcagagccacagttgtacagagtgcaaaggtttattgggtctctgtccaaagtcca
aaactttcatattctggaggagtcaggcttttggcccctgcactcagccagctactcctcggtacctctgtactgctgttggcgctgcttcactgtctga
tggctacggtctgacacaaactggaaacaatgccaagatgattgtcccactttatcaactaggttgtgtggtccttacagagaaaaattgtcaaggcata
tagcagtgataccccaggaaaaaggccagtgttaccggtgaaattgcctaaaatggttataaaaaatggattgccttcgcaacaccccctcttaaaaggg
cttgctccattgtaaaaataccctggaagtagaaaattttcaccccgggggtgttcctaacattttttttccccgggggaaaaccccgaaattataagtt
tggccagaaacccctgaattttctgttgggccccacaaac
Library:
Description: Bulk tissue was collected from a whole 10 month old male from the F6 strain. 1st strand cDNA was primed with a Not I - oligo(dT) primer, double-stranded cDNA was cloned into the Not I and EcoRV sites of pExpress-1. Library was size-selected for >1.5 kb fragments for an average insert size of 1.92 kb. Library was normalized to Cot5 with a 180-fold reduction of actin. A non-normalized version of this library is also available (NICHD_XGC_Swb1). Library was constructed by Open Biosystems (Huntsville, AL). Note: this is a Xenopus Gene Collection library.Vector Details | Vector Map |
Name: pExpress-1
Type: plasmid
5' Restriction Site: EcoRV
3' Restriction Site: NotI | |
Usage:
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