XB-CLONE-4358980
Clone Name: IMAGE:7025577
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Gene:
nit2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC091101 [+]
GGCAAAATTCCGACTCTCCTTGGTCCAGTTCCTTGTGTCTCCTGTGAAATCGGAAAACCTCAACAGAGCCTGCAAGCTAATTAAAGAGGCTGCACAAAAA
GGGGCTCAGATTGTTGCACTGCCGGAGTGTTTTAATTCACCTTATGGCACAAAGTATTTCCCTGAATATGCTGAGAAGATCCCTGGGGAATCCACTGAGC
GTCTGTCACAAGTAGCCAAGGAATGTGGAATCTATCTCATTGGAGGTTCAATCCCTGAAGAAGATTCTGGGAAGTTATATAACACATGTGCTGTATTTGG
ACCTGATGGAACCTTACTAGTTAAACACAGGAAGATCCATCTATTTGATATTGATGTTCCTGGGAAAATACGTTTCCAGGAATCTGAAACACTAAGCCCT
GGAGACAGCTTCTCTGTCTTTGAAACACCTTACTGTAAAGTTGGAGTGGGAATCTGTTATGACATTCGATTTGCTGAGCTTGCCCAACTATACTCCAAGA
AAGGCTGTCAGCTGCTGGTTTATCCTGGTGCGTTTAACATGACAACTGGGCCAGCTCACTGGGAACTTCTACAAAGAGCACGAGCTCTGGACAACCAAGT
ATATGTGGCAACTGCATCTCCTGCCAGGGATGAGAAGGCATCCTATGTGGCTTGGGGCCACAGCACAATAGTCAGCCCTTGGGGCGAAGTCATTGCAAAA
GCTGGTTCAGAGGAAACAGTCATTTCTGCTGACATTGATTTGGAGTATCTTGCTGAGATCCGGGAGCAAATACCTATTCGTAGGCAGCGCCGCCACGATC
TTTACAGTGTAGAAGAAAAGAAGAACTAAAGACTTTTTTCTTTTTTTTTTTTTTTAAAGAAAAAAAATAATTATTCTTCTAAATGTCATGATTTTATAAC
CTGACCAATACTCACAAACCCCATTGAAAGTTATGGTTATATATGCCAAGCCCACTTCTTGCAACCCATACCAGTGCTCATGAAACTTGGCAAGTTATGG
TGGTATGTGTCTGTCTTATTTCCTGCAGCCCAGAGTAGTGTTCATGAACCTCATCAGAAGTTAATGTATGGGAAGCTATAAATATATGTACCAAGCCCAC
TTCCTGAAACCCATACCAGTGCTCATGGGAATTTCTGGTTCAAGCCCACTTCCTGCAGTCCAGGCTAGTGCTCATGAACCTCATGGGAAGTTCTGGTTCA
AACCCACTTCCTGCAGTCCAGGCTAGTGCTCATGAACCTCATGGGAAGTTCTGGTTCAAACCCACTTCCTGCAGTCCAGGCTAGTGCTTATGAACCTCAT
GGGAAGTTCTGGGATTTATTTATGGCAAGTTAAGCGCCTAGCCCACCTACAACCCGTAACAGTGCTCACAGACCACATAGGAAGTTGTGGTTGTAGGTAT
GGGAAGGGCTCAAACACTTATAAGTTACAGTTTATATATTGTACAGAAACTGGAATGTTTAAACTAAAAATGACATTGAAGCAGAGACTAAATAATTCTG
AGAGTTGTAGTTTAGCAATACTGGGTAGGTTAACTCAGGTTGTTGTCCATACTGTATAGAACAGGTGTGGGCAAGTACATCTTTCAAAATCCAACCTATT
ATAGTCCAGTAATGTAAGTACAGTTCCTAGAATCTATCCGGGTGATTGGAATTGTAGTCAGCAGCATCTTGGTCTGCCAGTCACTCGTCGGCCATTTACA
GATTAAAGTGTTTGTCAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CF592963 [+]ggcaaaattccgactctccttggtccagttccttgtgtctcctgtgaaatcggaaaacctcaacagagcctgcaagctaattaaagaggctgcacaaaaa
ggggctcagattgttgcactgccggagtgttttaattcaccttatggcacaaagtatttccctgaatatgctgagaagatccctggggaatccactgagc
gtctgtcacaagtagccaaggaatgtggaatctatctcattggaggttcaatccctgaagaagattctgggaagttatataacacatgtgctgtatttgg
acctgatggaaccttactagttaaacacaggaagatccatctatttgatattgatgttcctgggaaaatacgtttccaggaatctgaaacactaagccct
ggagacagcttctctgtctttgaacaccttactggtaagttgagtgggaatctngtatgacattcgatttgctgagcttgcccaactatactccaagaaa
agctgtcagccgctgggttatcctgggggcgttttaaaaggaccaactgggggcccactccattgggggaaattcttccaaaaaaccccaaacccttggg
aaaaccaaatttttgttggggaaaatgggtttttccgggcagggggataaaaaaagggccccccattttggggttgggggggccccccccccaaaaattt
tccccccccttgggggggaaaaaacctttttggaaaaaaaaggggtgttccaaagaaaaaaaaaaaccttttttttccgcggcgaaattttttattttgg
gggaaaattcttttcggggaggaaaaaccgccgggggggaaaaaaaaaacttttttttttttggggaggaggcgccccccccccccccaaattttttttt
tgggggggggggaaaaaaaaaaaaaaaaaaa
Library:
Description: Bulk tissue was collected from a whole 10 month old male from the F6 strain. 1st strand cDNA was primed with a Not I - oligo(dT) primer, double-stranded cDNA was cloned into the Not I and EcoRV sites of pExpress-1. Library was size-selected for >1.5 kb fragments for an average insert size of 1.92 kb. Library was normalized to Cot5 with a 180-fold reduction of actin. A non-normalized version of this library is also available (NICHD_XGC_Swb1). Library was constructed by Open Biosystems (Huntsville, AL). Note: this is a Xenopus Gene Collection library.Vector Details | Vector Map |
Name: pExpress-1
Type: plasmid
5' Restriction Site: EcoRV
3' Restriction Site: NotI | |
Usage:
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