XB-CLONE-4358607
Clone Name: IMAGE:7026743
|
Gene:
nolc1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC077061
[+]
CCCGGCGGCGTGGCTGGTACTGTGAGAGTATGGACGGCTCTAGCGTGGTGCCCAGCGATCTCTTCCCGCACGTTCTGCGCTTTTTGCGGGAGAACAAGTT
TACTAACGCTGCCAACGAGTTCAGCAAAGCTACTGGGGTGAAAGACAATGATTCCTGTCCAACATCTCTTCTGAATATATTTAGCGACTGGATGAAGTCA
CCAGATGCAAAGAAGAGAAAGCCATCAGCTAATGGTTTGCCTAAGAAAAAGCCTACAAACGAGTCTTCCAGCAGCGAAGACAGCTCCAGTGAGGAGGATG
AACCTCCTGCAAAAAAAAATAGCAACTCAGTCTGCTGAAGGGAAGAAGCCTGTTGTGAAAGGCGTCCAAGCAAAAAAAGCAAAGAGCAGCAGCGAGGACT
CCAGTGATGAATCTGATTCTGAAAAAGAAACAAAGAAACCTCCTGTTAAAAAACCCGCACAAACACCAAAGGGAGCAGCTTTAAAAACACCAACTCAAAA
GAAGGCTGAGAGCTCCAGTTCTGAATCCAGCAGCTCAGAAGATGAAGCCTCTAAGAAGAAGAAGCAATCTGTGACTAAAGTCCCTCCAAAGCAGACAGTA
GTAAAGGCTGGTTTAGCAAACGACAATCAAAAAGCAGCAGACTCAAGTAGCAGTGAGGACTCTGACAGCCCCCCAGAAAAGAAGTCTGCTGCTCCAAAGA
CACCTCCAGCCAAACCCGCCACAGCAGCTAAGCCACAAGCAAAGAAGACAGCAGGGAAGAAGTCCAGTAGTGAGGACTCTTCAGACAGCTCAGATGAAGA
GGCAAAGCCTGCAAAAAGCAAACCAAAGCCAGGTGTCTATAGTGCTGTACCACCACCTACTATGAATTCTAAGAAAAAGGCTTCTTCACAGCCTGGCACC
AAAACTAAGGCGGAAAGCAGTGACTCTTCAGACAGCAGTGATGATGAACAGCCACCAAAAAAAAAAAAAAAA
5' EST: CF722991
[+]taattaatccccccccccccccccggcggcgtggctggtactgtgagagtatggacggctctagcgtggtgcccagcgatctcttcccgcacgttctgcg
ctttttgcgggagaacaagtttactaacgctgccaacgagttcagcaaagctactggggtgaaagacaatgattcctgtccaacatctcttctgaatata
tttagcgactggatgaagtcaccagatgcaaagaagagaaagccatcagctaatggtttgcctaagaaaaagcctacaaacgagtcttccagcagcgaag
acagctccagtgaggaggatgaacctcctgcaaaaaaaaatagcaactcagtctgctgaagggaagaagcctgttgtgaaaggcgtccaagcaaaaaaag
caaagagcagcagcgaggactccagtgatgaatctgattctgaaaaagaaacaaagaaacctcctgttaaaaaacccgcacaaacaccaaagggagcagc
ttttaaaacaccaactcaaaagaagggtgagagctcccgtttctgaatccagcagctcaaaaagatgaagcctctaaaaaagaaaaagcaatctggtgac
ttaaagtccctccaaaaagcagacagtaagtaaaaggcctgggttttagcaaaacgaccattcaaaaaaagcaagcagaacttcaaagtaacccattgga
ggggatttcttgaaaaggccccccccaataaaaaaaaaaatcctggcctggctcccaaaagaacacccttccagagccaaaaccccgggccaaagcacag
ctttaagcccccacagcccaaaaaaaaaacagcccgggggaaaagaaattccccacttaagttgggggaaactcctttttaaaaaagctttaagaattta
aagaaggcctaaaacccctgttctaataaagccataacccataaacgcccagggtgttctatataaggggcgtgtatccaccccccccctcattttgtga
tattccaaaaaaaaaaaaaaaggccca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: