XB-CLONE-4358552
Clone Name: IMAGE:7026531
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Gene:
golm1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077706
[+]
GACTGATGTTGCCCGGCAACGGCTTAAAGTGAAAAGCCCAGTACAGAGTTCCTGGAGTCCAGTACGTGCGGGAGAGAAGGGGTGTGTGCTAGTTAAGCAT
GAGGTGTTGGGCATAGGGAAACGAGCTGGCAGCTAGCTAAAGGTAAACTGCACCTAGACGGGAACAAGCTGTGCGGCCTTAAAGCCCAAGATCTGGAGCT
CCTGCAGAATGATGGGATCAGGCAATGGGCGTAGAGGAATGAAGTCACCACCTCTTCTTATAGCTGTACTATTGGCATGTATTTTTGTTTTAACAATAAA
CTACTGGCTCACAAGCTCAAGATGTGCCGAACTGCAGAATCAATTGGCAGACCTAGAGAGCAGGATGCATAGGATAGCTGCGGAACGTGGAGCAGTTGAG
CTGAAGAAGAATGAATTCCAGGACAAGCTAGAAAGGCAGAAGAAACAGATTGACACTATCCAGTCCTTGCATAACTCCCAGTTGCAAAATGCGAATGAGG
CGTGCCACAATGAAAAGGAGACATTATTAGATACCATATCAGAGAAAGAAAAGGTTATGCAGGATCTATATGGGCAGATCAAGACTCAAGAACAAAACCT
GTCAAAATTAAAACTTGACTTTGAACAACTACAGGAAAGCCAGGCTAAGAAATCCACATTCGAGCTAGCCCAGTGCAGCTATAAAATGAAGGAACTCAGT
GAGCAGTGTGAGGAGAAGTTGCGGAGAGCCTTAGGTGGAGGTGGGACTAAAGCACAAGATGAAAATGCTGATACTCAAAATACTCCCAAGCCCATTATAC
TAAAAAAAAAAAAAAAA
5' EST: CF723036
[+]taattaatcctccccccccccgactgatgttgcccggcaacggcttaaagtgaaaagcccagtacagagttcctggagtccagtacgtgcgggagagaag
gggtgtgtgctagttaagcatgaggtgttgggcatagggaaacgagctggcagctagctaaaggtaaactgcacctagacgggaacaagctgtgcggcct
taaagcccaagatctggagctcctgcagaatgatgggatcaggcaatgggcgtagaggaatgaagtcaccacctcttcttatagctgtactattggcatg
tatttttgttttaacaataaactactggctcacaagctcaagatgtgccgaactgcagaatcaattggcagacctagagagcaggatgcataggatagct
gcggaacgtggagcagttgagctgaagaagaatgaattccaggacaagctagaaaggcagaagaaacagattgacactatccagtccttgcataactccc
agttgcaaaatgcgaatgaggcgtgccacaatgaaaaggagacattattagataccatatcagagaaagaaaaggttatgcangatctatatgggcagat
caagactcaagaacaaaacctgtcaaaattaaaacttgactttgaacaactacaggaaagccaggctaagaaatccacattcgagctagcccagtgcagc
tataaaatgaangaactcagtgagcagtgtgaggagaagttgcggaanagccnttanngtggaggtggggactaaagcacaagatgaaaaatgctgaata
ctcaaaaatactccccaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: