XB-CLONE-4358403
Clone Name: IMAGE:7028225
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Gene:
crhr1.1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC084149 [+]
GATACTGGAAGCCTGGATCTGTCTGGTCGCTGACTTAGGTGTCCTGCGATATCACAGAGATGACGGAGGAAGCCTGGAGACTCCTGATATATGTTACTAT
GTTGGTTACATGTCCTATAAAGGCTGCGGTGGACGCTGATGATGTCATCACCAGGGAGGAACAGATATATCTTCTGTCTCGGGCAAAACGTCTCTGCCAG
ACAAGTATGACAACGCAGGCCACTGGAGCAATACGTAACACATCTAAGGGGCTGAGTTGTCCTCCTGAGTGGGACGGTATAATATGCTGGCCCCGTGGAT
CCCCCAACGAGCTTGTTTCCGTTTCTTGCCCTGAACATATCTATGATTTCAACCATCAAGGATTTGCCTACCGGAAATGCGGGCAGTCTGGGACTTGGAT
TCAGGTGCCAGGGCTTAACAGAACCTGGGCAAACTACAGCGAGTGCCTTACATGGGTCGGCTCAGGACACAGAGCAAAAGAGAAGGAGCTGTTTGAGCGA
CTGCGGTTGATTTACACCATAGGTTACTCCATCTCTCTGGCTGCTTTGTTATTGGCCCTTTGCATTCTCTGCTATTTTAAGCGCCTCCATTGCACCCGGA
ATTACATTCACATCCACCTTTTTGCATCATTCATATGCCGAGCAGGGAGCATATTTCTGAAAGATGCCATTTTGTATTCAGGGGAGAATGAAGGGGCAGC
CTTGGATGAGAAGATAGAGTTTGGCCTGTCAAAGAGAACAGGGCTCCAGTGGGCAGGCTGCAAAGTGACTGTTACACTATTCCTTTATTTCCTGGCCACT
AATCACTACTGGATCCTGGTGGAAGGACTTTATTTGCACAGTCTGATCTTCATGGCGTTCCTGTCTGACCGTAACTACTTCTGGGCGCTGATTATCATTG
GGTGGGGCTTGCCGGCTGTGTTTGTATCAGTGTGGGCGAGTGTCCGGGTGTCTCTCGCAGATACACAGTGCTGGGATCTGAGTGCTGGACACATGAAGTG
GATTTATCAGACTCCCATTTTGGCAGCAACTCTGGTAAACTTCATCCTGTTTCTCAACATAGTCAGAGTTCTGGCCTCCAAGTTGTGGGAAACCAATAAC
AGAAAGCAAGACCCATGTCAGCAGTATGGGAAGCTCTTAAAGTCTACGTTGGTTCTCATGCCTCTCTTCGGAGTACATTATGTGCTGTTCATGGCGATTC
CTTACACAGAGGTGACTGGGCTGCTGTGGCAAATACAAATGCACTATGAGATGTTCTTCAATTCTTCCCAGGGATTTTTTGTTGCTATTATCTACTGCTT
CTGCAATGGAGAGGTGCAAGCTGAGGCCCGTAAATTTTGGTTCCGCAGAAATCTAGCAGTCGATCTCAAGCAGAAGTCTCGTATGACCAGCACCGGAGGC
AGTTGCCATTATGGAGGAGTCTTGACCCATACAACAAACAGTGTGTGTCTGAGTATGGTGGCCAGGGGCAAACAGATGGCATCACTCCAGTCCTCGGCTC
ACCTCCCTGGGTACATTTCCAACACAGTCACTTCTAACAGCACCACACAGCAACTGGGCAATGTCATCTAGGGACAAGGGTGCTAATTTGTCATTTTATA
GGACATATTCATTTCTCCAACCATAAATAATGTACACAGGTCACTTTATTATAAGTAATATGAACTTTTAAGATGATCATAAACCAGAACATGATATGAC
TGATAGGCCCATGATCTACGTGCATCAATATCATATTATTAAAAAAAAAACAAACCATACGGATGCTTGTAGGAGGCAAGCTTTGCTTATTGAACAAACC
AGCTGCTTGCATTTGTCTTCAATAAAATAAAGATTAAAGTAGAGACAGCATTTAATCAAGTCATCTATTATTAATACTTCCTTTTGGGCTTCTGCAATTT
GCACAGCAAAGCAAGGAAAAGAATTGTGATACAACATTAAAGAGATTGCTGTTTCATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAA
5' EST: CF780983 [+]taattaatcccccccccccccgatactggaagcctggatctgtctggtcgctgacttaggtgtcctgcgatatcacagagatgacggaggaagcctggag
actcctgatatatgttactatgttggttacatgtcctataaaggctgcggtggacgctgatgatgtcatcaccagggaggaacagatatatcttctgtct
cgggcaaaacgtctctgccagacaagtatgacaacgcaggccactggagcaatacgtaacacatctaaggggctgagttgtcctcctgagtgggacggta
taatatgctggccccgtggatcccccaacgagcttgtttccgtttcttgccctgaacatatctatgatttcaaccatcaaggatttgcctaccggaaatg
cgggcagtctgggacttggattcaggtgccagggcttaacagaacctgggcaaactacagcgagtgccttacatgggtcggctcaggacacagagcaaaa
gagaaggagctgtttgagcgactgcggttgatttacaccataggttactccatctctctggctgctttgttattggccctttgcattctctgctatttta
agcgcctccattgcacccggaattacattcacatccacctttttgcatcattcatatgccgagcagggagcatatttctganagatgccattttgtattc
aggggagaatgaaggggcagccttggatgagaagatagagtttggcctgtcaaagagaacagggctccagtgggcaagctgcaaagtgactggtacacta
tttctttattttcctggccactaatcactactgggattctggtgggaggaactttatttggccagtccgaatctttatgg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: