XB-CLONE-4358327
Clone Name: IMAGE:7028150
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Gene:
hhatl
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC081363 [+]
GAGTGCCTCGGTGCCTACATGGATCCCTGGGGCTGCCCTGTCCCATTAGCCCTCTGTGCCCGACACTGATCTCCTGCCCGACTCCTGCCCAGCTCCTGCC
CGACTCCTGTCCGACTCCTGCCCGGGCTCCTACACAGCATTCCAAGAATTGGATCTCTGAGCCGTCTGTGCCGCTAAAGCCGCACCATGGGAATCAAAAC
GGCGCTGCCGCACTACGAGCTGGGCTTCTATGCCCTCGTGGTGACTTGTGCCTTGGTCTACTCCCTCAATGGAATAATCGAAGCCTCCAGGGATGGAATG
AATCGGAAGTCATTTCGGGAGAACATAAAGGAGGGCTGGCATTACTTCGGGAGGAAAATGGACGTTGCCGACTTCGAATGGGCCATGTGGTTCAACACAT
TCAGGAACTACATTATCTTTGCCCTTTCGGGACACGTCATCTTTGCCAAGTTCTTTTCGCTAATGGTTCCTCAGTACAGATCATACGTTTACATGCTGTA
CGGGATCCTGACGGTACTGGCGATCATGGGAACCAACTACCTGATGCTCATCTTGTCCCACTGTTTCTTCCTCTACACCATTTCGCTGGTTAAAAGCAAG
TGGCTGTGCTTTGCTGCAGGCCTCTGTAGCCTGGCCACCTTCAAAATGGAACCGTTCAACACTTGGCAAAGTGGCTTTGTCACGGGGACGTTTAGCCTCC
AAGACATTCTCTTTTACGGAGGCAGTGGATTTTCCATATTGCGCTGCATGAGCTTCGCTCTGGAGACCTGCGAGAGGAAACAAGGGTTCTACTCCCCGAT
GGAACTTCTCAAATACAATTTCTACCTCCCTTTCTTTTTCTTTGGCCCTATTATGACTTTTGACCAGTTTCACGTGCAGGTGAACACAAGAGTCCTGACT
CGGAGGGAGAATGAGATGTGGAACATCCGAGTCAATGCTCTGACGCAGATCCTAGTCATTCTATTGGTGGACGTGTTCTTCCATTACCTGTACATCCTGA
CCATCCCCTCGGACCTGAAGTTTACCAACCGCCTTTCTGACTGGTCACTGGCGGGCTTGGCCTACTCCAACTTGGTGTACGACTGGGTGAAAGCCGCCGT
GATGTTCGGAGTTATAAACACCATCTCCAGGCTGGACCACCTCGACCCCCCACAGCCGCCCAAGTGTATCACCATGCTTTATGTCTTCGCGGAAACGCAC
TTCGACCGTGGGATCAATGACTGGCTCTGCAAATACGTCTATGACTACATCGGGGAAGGCCACGATAACATAAAGAGGGAGTTAATAGCCACCGTGGCTA
CTTTCCTCGTCACTACTTTATGGCTCGGCCCGTGTGAAATCGTTTATATCTGGTCCGTCTGCAACTGCTTCGGCCTGAACTTTGAACTCTGGGTGCAGAA
ATTCTTCCAGCTGGGACCCTTTGCAAGATTTGAGGCCATGTTGCCAGAGTCCATGTCCAGGAGAATCAGAGGGGTTTTCGGTGCAGCCAACTTCTGGGCA
ATTATTTTCTATAACATATTGGCCCTAAACAGCCTGGACTTTGCTCTGCTGGTAGCCAAGAGGCTTCTAATTACAGGTTTCCCAGAAAGCACCCTGTCCA
TCTGGCTTGTTACCTACTGCGGGGTGCAGCTGATAAAGGAGCGAGAGCGGCTAATGGCCATCGAGCAAGAGAGGCTTGAGAAGCAGAAAGTCGCTTAGTC
GCTGCGTTTGTTCGTAGAAACCAATGAAACGCAAAAGAGCAGCGTCCTCTGTGCCGGGGATATCAGCGCTTAGCTACACGGGAAGCTGAGAGTTGTAGTT
CAGCCGGCACCGGAGGGCAGGCAGGTTCCAAACAATCTGCAACCGGCCCTTATATTTGTCTGTATAGGAACCGATATGCCACAGGGAAAGTAGCTTTAAT
AAATATTTTACTATTATAAGTAAAAAAAAAAAAAAAA
5' EST: CF781021 [+]taattaatcccccccccccccgagtgcctcggtgcctacatggatccctggggctgccctgtcccattagccctctgtgcccgacactgatctcctgccc
gactcctgcccagctcctgcccgactcctgtccgactcctgcccgggctcctacacagcattccaagaattggatctctgagccgtctgtgccgctaaag
ccgcaccatgggaatcaaaacggcgctgccgcactacgagctgggcttctatgccctcgtggtgacttgtgccttggtctactccctcaatggaataatc
gaagcctccagggatggaatgaatcggaagtcatttcgggagaacataaaggagggctggcattacttcgggaggaaaatggacgttgccgacttcgaat
gggccatgtggttcaacacattcaggaactacattatctttgccctttcgggacacgtcatctttgccaagttcttttcgctaatggttcctcagtacag
atcatacgtttacatgctgtacgggatcctgacggtactggcgatcatgggaaccaactacctgatgctcatcttgtcccactgtttcttcctctacacc
atttcgctggttaaaagcaagtggctgtgctttgctgcaggcctctgtagcctggccaccttcaaaatggaaccgttcaacacttggcaaagtggctttg
gtcacgggggacgtttagcctccaagacattctctttttacgggaggcaagtggatttttccatattt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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