XB-CLONE-4358299
Clone Name: IMAGE:7028131
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Gene:
pno1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077670
[+]
GTCTCTGGGACCCGGATGGCTGCTGAGATGCACGTGTAGTGATATCGCTGGGAACTATCGGAATAGAGTTTTTTTCTATCGCTTCTGTTTGGAATAAATC
ACATCGCAAGAATGGAGACCGAAAGCGCCGCAGCCTCCGGGGAAAGTTTCACAAGTGTCACCTCTAAAAAAACCAGGAAGAGAAGAGCGGCCGAGGAGAG
GCCCATGGACACCGGGGAGCAGCCGAGTAAGAGGCCGGACTTTCCTCCCATCTCTGGGGATAAGCTGATGGGAGACAAAGATGAAATGAGGAAAGTCCCC
GTCCCCTCACACAGGTACACTCCTCTTAAAGAAAACTGGATGAAAATCTTCACTCCGATTGTGGAACACTTACAGCTTCAAGTGCGGTTCAACCTAAAGA
CAAGAAATGTAGAAATAAAGACTTGCAAGGAAACCACAGATGTTGGCGCATTAACAAAAGCAACTGATTTTGTCAGAGCCTTTATTCTGGGGTTTCAGGT
TGAGGATGCGTTAGCCCTGGTCCGGCTCGACGATCTCTTCTTGGAATCATTTGAAGTCACAGATGTTAAACCGCTGAAGGGAGACCACTTGTCTCGGGCC
ATTGGGCGGATAGCTGGAAAAGGAGGCAAGACAAAGTTCACCATAGAAAATGTCACAAGGACAAGAATAGTTCTTGCTGATTCAAAAATACACATACTGG
GATCCTTTCAGAACATCAAGATGGCCAGGACTGCAATCTGTAACCTTATCTTAGGAAGTCCTCCATCCAAAGTGTACGGCAACATACGGGCAGTGGCAAG
TCGCGCTGCCGATAGGTTCTAACATTGCGTGAAGTGGAACGACTGAACATCCTATCAATGTGGCCCCACTTACATCTACCTATGTGCAATGAAACGGCGT
GCAGAAGAGAAGATCTCATTGGCAGAATCTCTGGCAACATTTACATCAAAGAACCAGCTGGTTGGCTCAGACTTGTCTGGCTCTTCCCATTAATATTTAA
GTGGTGTTAAAGACTTGAACCTATAAAGGATTACACCTCTGCCAATACACTGTGAAAATAAATCAATAAATACAAAAAAAAAAAAAAAAAAA
5' EST: CF781035
[+]taattaatcccccccccccccgtctctgggacccggatggctgctgagatgcacgtgtagtgatatcgctgggaactatcggaatagagtttttttctat
cgcttctgtttggaataaatcacatcgcaagaatggagaccgaaagcgccgcagcctccggggaaagtttcacaagtgtcacctctaaaaaaaccaggaa
gagaagagcggccgaggagaggcccatggacaccggggagcagccgagtaagaggccggactttcctcccatctctggggataagctgatgggagacaaa
gatgaaatgaggaaagtccccgtcccctcacacaggtacactcctcttaaagaaaactggatgaaaatcttcactccgattgtggaacacttacagcttc
aagtgcggttcaacctaaagacaagaaatgtagaaataaagacttgcaaggaaaccacagatgttggcgcattaacaaaagcaactgattttgtcagagc
ctttattctggggtttcaggttgaggattgccgttaaccccgggtccggctcgacgatctcttcttggaatcatttgaagtcacagaaggttaaccgctg
aagggagaccacttgtctcgggccatttggcggatagctgggaaaggaggcaaaacaaatttctccttagaaaatgtcccaaaggacaagaatagttctt
ggggattccaaaatacccttccggggatccttttcgaaaaatcaagatggccagggaaggcaattctgtaacctttatcttaaggaaggccttccatccc
aaaggggtacggggaaatattagggggacgggggaaagattccggcctggccaaaaaaggttcctaaaattggcgggtgaaaggggaaaacaattgtaac
aatccctatacaagaggggcgcgccacattttatatttttcacctatg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: