XB-CLONE-4358231
Clone Name: IMAGE:7028062
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Gene:
lmbrd1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080477 [+]
AGGAAGTTGAGAAATGGCGACGGGGAGCACCGAGCTATTGATAGGATGGTGTATCTTCGGGGTGCTGTTACTGGCTATTTTGGCCTTTTGCTGGGTTTAT
GTCCGAAAATACCAAAGCCATCAGGAAAGTGAAGTGATTTCAACTATAACAGCGATATCATCACTAGCTATTGCACTCATCACTTCAGCACTTTTGCCTG
TGGACATTTTTCTTGTTTCCTTTATGAAGAATCATAATGGAACATTTAAGGACTGGGCTGAAAGCAATACCACACGATTGCAAATAGAGAACACAGTATT
AATTGGATATTACACTTTGTATTCTATCATACTTTTCTGCGTGTTCTTGTGGATACCTTTTGTGTACTTCTATTATGAAGAAAAGGATGATACTGATGGA
AGCCAGTGCTCTCAAATAGCCAATGCTTTCAAGTACACATCTGGGTTTATTCTAGTCTGCTCATGTCTGCTGCTCATTGGAGCTTTTGCTCCCTTGGATA
TCCACACGAACAAAAACTCCACAGACCTGGATAAAATTAAGCTTTTATTTCTGGAACTTGGGAGCAGCAATGGTTTGGCTGCCTTGTCATTTTCCATTAG
TTCACTGACTTTGATAGGAATGCTAGCAGCGATCACCTACACAGCTTATGGGATGTCTGCATTACCATTAAATCTAATTAAAGGTACAAGAAATGCACAT
TATGAACGCTTAGAAAACTCTGAAGATATTGAAGAGGTGGAACAACAAGTGGAAAGGATTATGTCAAAGTGCAAGGATGGGAGACCATTATCTAGCAAGG
ACAGACAAGCCCTATACAAACTTAAAGAAAAGCTGAGGACATTGAAAAGGAGAGACCGGCATCTTGAATATCATGAAAATAATTGCTGGACAAAATGTTG
CATTGTCATAAGACCATTTAAGATTATTTGGGGAATCCTTTTCATCCTGGTGGCTCTTTTATTCATAGTTTCACTTTTTCTCTCCAATTTGGATAAAGCG
CTTCACTCTGCTGGTATTGATTCTGGTTTTATCATTTTTGGTACCAACCTAACAAATCCTTTGAATATGCTCTTGCCTGTTCTGCAAACAGTGTTCCCTC
TTGACTATATCTTTATTACAATCATCACAATGTATTTTATTTTCACATCAATGGCAGGAATACGAAACATGGGCATATGGTTCTTTTGGATAAGGTTATA
CAAAATAAGAAGAAGGCGGACTCGACCACAAGCTCTTCTGTTTCTGTGCATGATCCTTCTTTTGATTGTTCTACATACTAGTTACATGATTTACAGCCTT
GCACCGCAGTATGTGATGTATGGCAGCCAGAAGTATCTATGGGAGAACAATAGCACACAAGAGACTGCAATAGGAAATTCTTCTGCCTCTGTTTTGAAAG
ATTGTGATGCCAGTGCTCCTGAGGATCAGTGCACAGTTACTCGAACATACCTGTTTCTTCACAAGTTTTGGTTTTTCAGTTCCATCTATTATTTTGGAAA
TTGGGCTTTTATTGTGGTATTCGTTATTGGATTAATTGTGTCCTGCTGTAAAGGGAAAAAATCCGTCATTGAAGGGGAAGTGGAAGATGATGATTCTGAC
CTAAGTGATGATGAAGAGCATCCATATGCTTAAGCCAATGTACGTGATTATGTAACAAATTCTAAAAACTGTTTCGTTTCAGAAAACAAGAGAATATTTT
AAAATAATGGGATAGAATCTTTGTGAATTCACTCATGGAATGCGGGTCTGCTGATAACTGTATGGTATAAGAAAGGTGCAAAGAAAGATTGCTGCTGCTT
GATCTCTGCAATGTATTAAAATTATAACATGTCAAATTATACAATTTGTTTTACAAATCTACTGCATTAATTTAACTTTGCTTGTGTTTGCAAAATAAAT
ATGTTTTCTGCAGGGTGATGGCATTCCATTATTTAGCTCTGCCTACAAAAAATATTTTGTTTGAACTTTCCTTATACAGGCGATCTTTGTTTGATTGTTA
TATTTACATCAATTAAAAATATAATATTTTTCTATTGTCAATTAAACAGTGTTTTGCCTTGAAAAAAAAAAAAAAAA
5' EST: CF781069 [+]taattaatcccccccccccccaggaagttgagaaatggcgacggggagcaccgagctattgataggatggtgtatcttcggggtgctgttactggctatt
ttggccttttgctgggtttatgtccgaaaataccaaagccatcaggaaagtgaagtgatttcaactataacagcgatatcatcactagctattgcactca
tcacttcagcacttttgcctgtggacatttttcttgtttcctttatgaagaatcataatggaacatttaaggactgggctgaaagcaataccacacgatt
gcaaatagagaacacagtattaattggatattacactttgtattctatcatacttttctgcgtgttcttgtggataccttttgtgtacttctattatgaa
gaaaaggatgatactgatggaagccagtgctctcaaatagccaatgctttcaagtacacatctgggtttattctagtctgctcatgtctgctgctcattg
gagcttttgctcccttggatatccacacgaacaaaaactccacagacctggataaaattaagcttttatttctggaacttgggagcagccatgggttggc
ctgccttgtccttttccattaattccctgaactttgataagaaatgcctaccacccaatcaccttacacacgccttatgggggatggtctgccttttacc
catttaaaattccttattttaaaggggtaacccagaacaattggcccccttttaattgaaaacccctcttaaagaaaaaaccttcctgtaaaaaaaataa
tttgtgaaaaaaaaggc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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