XB-CLONE-4358217
Clone Name: IMAGE:7028053
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Gene:
hbp1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC081362
[+]
GAGAGATTAGGTAATGGCGACGGGTTTGTTCAAGTGCTATAATATGGTGTGGGAAGTCAAGGCAAATGATATGCCCAACGCTGTGCAAAAGGTCCAGTTG
GTAATGGATAACAGACATACAGGAATAAGTGAATCTTTGGAAATGTTGAAATGCAATGAGCATATTCCGTCCTCTCCTGGGTATGAATCGTCTGATGATC
ATATGGAGCTTGATAACAACATGATGGGGCACATTCATCAAAGTACAACTTCGAATCCTGAAATGGATGATATTCCAGAATTGCGAACTGTGCCAGCCGA
TTCAACATCACCTGAGGTTTTCCACCTTGACTCGGGTATTTCACACCAAGAATATACAGTGCCTTCATGGAGCCAAAATACCTCAGACATCTCTGAAAAT
GAATATTCGTGTGATACTGGCATGAATTGGCTGACTGAGCTGGCAAATATAGCCACAAGCCCTCAGAGTCCTTTGATGCAATGTTCATTCTATAACCGAT
CATCACCTGTTCATATAATAGCTACGAGTAAAAGTTTACATTCCTATGCCCGCCCCCCTCCTGTTCCCACAGAAAGCAAATCAAACGTCTCCAGAAGTCA
TTGGCGAGAGGAAACCATGGTCAGACACGAACGAACAAACAGTGAATCAGAATCTGGCATTTTCTGCATGTCCTCATTTTCTGATGATGATGATTTAGGA
TGGTGCCACTCTTGGCCGCAGACAGTTTGGCACTGTTTTCTGAAAGGAACCCGTTTATGCTTTCATAAAGGGCGCAAAAAACAGTGGCAGGATGTTGAGG
AGTTTGCCAAAAGCACACGATGCAGAAATGAGAGGGGCATTGACTCATACAAGGGTTATGGTTCTGATGGTCTCAAGTTGGTCTCTTACGAGGAGTGCGT
ATCTTATGGAGAATCAGTCCTACAGCTTACTTTTGATCCTGGTACAACAGATGGAGGCTTGCTAACAGTGGAATGCAAACTGGACCACCCATTCTATGTG
AAAAATAAAGGTTGGTCATCTTTCTACCCAAGTCTAACTGTGGTTCAGCATGGTATTCCCTGTTGTGAAATTCATGTTGGAGATGTATGTCTACCTCCCG
GACACCCAGATGCCATCAATTTTGATGATTCTGGTGTTTTTGATACTTTTAAAAGTTATGACTTTACCCCAATGGATTCTTCTGCTGTGTATGTGTTAAG
TAGTATGGCACGTCAGCGCCGTGCTTCATCTAATGGAAGAGCAAGCAGTCCAGAGTCAGAGAGATCAGAATATTGCAAAGATTATGCATCTCCCAGATCT
TCACAGACCTCCTGTAGCTCATTATGTAATAAGACTGGCAGGAATCACACCTCAGGAACTGCAAACTCTGTGCCTGTCACATCACCCAACAAGTGCAAAA
GACCAATGAATGCATTCATGCTTTTTGCCAAAAAGTACAGAGTTGAATATACTCAGATGTACCCAGGAAAAGATAACAGAGCCATAAGTGTGATACTTGG
TGACAGGTGGAAAAAGATGAAGAATGAGGAAAGGAGGATATACACTATAGAAGCAAAAGCTCTGGCTGAAGAACAAAAACGTTTAAATCCTGACTGTTGG
AAGAGGAAAAGAACAAATTCAGGTTCCCAACAGCATTAAACAAGAATGTTGCTGAATCCTTGTGGAAGTGCTGTGTGTCCGCATCAATGAGAAAGAAAGA
AAGAAAAACACACTCACGATTATTCTCAGTCTTTGTGAGCAGAAGAGATTCTGGCAGAAAAATGGATTGAATGAGTGAAGGTTTTTTTTTAAAGAAAAAA
AAATTATGTATAGCATTAAGTATGCAAAATATCAATATTTTAAACCAAATTGCCTTATTTTTTGAAAATTTATGTTATCAGGTAACAGGCTTGAAAATTG
ATATCCTGTGGTGCTAAATGAGGTGTGTATCAGCCTATTGTTTGACTACAGATAAAAGGGGAACTTATAGAACATATAACTGCTGTTTATTGACCCTACT
GCTTATTAATATGTATTGCATAAATTATCTCTGACGTTAGAATTTGTATTTTTCTGACGTGATATTTTAATGCAAAGTGCATGAGCACTAACTGACTATC
CCGCGCCACTGAGCTTCTTGTACTTGCCGGGCGGATCATTCGCTTCCTGGAATTCATTCAGACCTCACCAGAGGACAAGGTGTTAAAAGCATATGGCTTA
GTAATGTAGTTTGCTGAAGAATCTACAAAGATTATATTTTGCTAGAGAAAATATCATGTACAATGTGGGGCTGACCTTGCACTTATCACTGGTGCAACAC
TTGCACTTTACTTTTCTTGCAACTGTCAGGTTGAACAGCTGCATTTCTTTGTTTGGTTGTAGCCCCAGGGCTATGAACTGCTGCAATCATGGCTATTTGT
GTTACTATTCAAGAATCACAGCTGGAAAACAGTTTGTCTGGAACTTGTGGAAACATAATATTGCCCCTATAATTTTTGCACACTATATTCTTGTATATTT
CAAATAAATATTGTTTAACTAGTGCTCTGGATTCATACGTTAATGTGCAATAAAAGGTAAAGTTGTCTTTGCAAAGAAAAAAAAAAAAAAA
5' EST: CF781076
[+]taattaatcccccccccccccgagagattaggtaatggcgacgggtttgttcaagtgctataatatggtgtgggaagtcaaggcaaatgatatgcccaac
gctgtgcaaaaggtccagttggtaatggataacagacatacaggaataagtgaatctttggaaatgttgaaatgcaatgagcatattccgtcctctcctg
ggtatgaatcgtctgatgatcatatggagcttgataacaacatgatggggcacattcatcaaagtacaacttctaatcctgaaatggatgatattccaga
attgctaactgtgccagccgattcaacatcacctgaggttttccaccttgactcgggtatttcacaccaagaatatacagtgccttcatggagccaaaat
acctcagacatctctgaaaatgaatattcgtgtgatactggcatgaattggctgattgagctggcaaatatagccacaagccctcaaagtcctttgatgc
aatgttcattctataaccgatcatcacctgttcatataatagctttcaagtaaaagtttacattcctattcccgccccccccctcctgttcctcacagaa
agcaaattccatgttctccctaaattcatttgtgcgataggaaatccttggtccagaacacgacttaatcaacccgtgaaattagactcctgggcatttc
tccgcagtgtcctccatttttccgatggatgaatgtctttgggatgggtgccacctctttggccttcaaaaaaagttttggcaactggtttccctgaaaa
agggaccccgcttttatgct
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: