XB-CLONE-4358089
Clone Name: IMAGE:7028427
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Gene:
ube2z
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080480 [+]
GGCAGACACTCTGGTTGTTGTCTCTATGTTGGCTGTACTCTCTCTTCCCCGTAGTCTTGTTCCTCTGGAGTCCCGGAAGTAAGTGAGAAGCGGAATGGCG
GAAAGTCCAGCGGCTGAAGCGATTATCCTCCCAGGAGCTGCGGCGGGTGGGGGTGTCCTTCCTCATCTGAGCGGGCTCCAAGCGCCTGGGACATCTCCTC
TGACAACCTCCTCGGTGTGGGACCCGACAGCCAGCGCCGACTGGGATAACGAGAGGGCATCTAATCAGTGCGTGCTGCGGATAAAGAGGGATATTATGTC
CATTTATAAAGAGCCGCCCCCTGGTATGTTTGTGGTGCCAGATCCTCATGACATGACAAAGATCCATGCACTCATCACAGGCCCGTTTGATACACCTTAT
GAAGGTGGTTTCTTCTTGTTCCTGTTCCGGTGTCCCCCAGACTACCCAATACACCCACCTCGGGTTAAATTGATGACAACAGGGAATAATACTGTGAGAT
TTAACCCCAATTTCTACCGCAATGGCAAAGTCTGCCTGAGTATTCTAGGAACTTGGACAGGACCTGCATGGAGTCCAGCTCAGAGTCTCTCTTCAGTGCT
CATATCAATCCAGTCTCTTATGACCGAGAATCCCTATCACAACGAACCCGGCTTTGAGCAGGAGAGACACTCTGGCGATAGCAAGAACTACAATGAGTGC
ATTCGACACGAGACCATTCGGGTAGCAGTGTGTGAGATGCTGGAGGGCAAGTGCCAGTGCCCTGATGCATTAAGGAGTGTTATGGAGAAATCATTTATGG
AATATTATGACTTTTATGAAGCAGTTTGCAAAGATAGATTTCACCTACAAGGGCAAAACATGCAGGATCCATTTGGTGAGAAGCGAGGCCACTTTGATTA
TCAGTCTTTGCTGAGCCGCCTTCAGACCATTCACCAGCGAGTGAGGGAGAAGCACCGGAAAGAGACTGTGGATATAGACTCTGATTCGAGCTCCTCAGAG
ACAGAAACTGACACACAGGGAAGCTCAAATCCTTAAGTGGATGGGAATGTAACTGTGTAGCGCTTGGTGTGTCGTCACTTCAGACTGTTATGTTAGCCGA
GTATCATGGATTGTGTTTGACAAGAAAGGACCTTGCTGACACCTCGCCTCTTTGCCTTGGATGCTGTATGGATGCAGTGTCAAGTAATCACTCTAATTAC
TAAGGAGCAAATGGGAAAAGCTTTTATGGAATGGATTCCGAATCATGGAGCGTTTGGATTTCTCGCCAGGGCAGCACTTTGTTACAATGTATGATTTTCT
CTCCTCTTTGCAGAATAGTCATGTATTGGTCCCGATGAGCCAAAGAGTCTTGACAATAGGAGAGCAGAATTCAGCCACGTGATCTCAAGCACATGAGAAA
TGCCATTTTTTATTTTTCAGATTTGACAGAAGCCACTACTTTTTAATTGTTTTGTTTTTTGTTTTTTTTTGTTCACCCCACAAAGAAATGCACTTTGTCT
TTTGCCTTCTGGGCTTTGTCTGTGTAGATGTTGTACATATGTTTGCTAGGGGAATAAACCAAGGCTTCTTAGATAAGTTCTTAGCACCCCCCACCCACTA
ATATCAGGGTCCCTTAATAGGTGTTAATTTATGAAGAGCAAATATAATCTGTAAATACAAAAAAAAA
5' EST: CF781140 [+]taattaatcccccccccccccggcagacactctggttgttgtctctatgttggctgtactctctcttccccgtagtcttgttcctctggagtcccggaag
taagtgagaagcggaatggcggaaagtccagcggctgaagcgattatcctcccaggagctgcggcgggtgggggtgtccttcctcatctgagcgggctcc
aagcgcctgggacatctcctctgacaacctcctcggtgtgggacccgacagccagcgccgactgggataacgagagggcatctaatcagtgcgtgctgcg
gataaagagggatattatgtccatttataaagagccgccccctggtatgtttgtggtgccagatcctcatgacatgacaaagatccatgcactcatcaca
ggcccgtttgatacaccttatgaaggtggtttcttcttgttcctgttccggtgtcccccagactacccaatacacccacctcgggttaaattgatgacaa
caggggaataatactgtgagatttaaccccaatttctaccgcaatggcaaagtctgcctgagtattctangaacttgggacaggacctgcatggagtcca
gctcagagtctctcttcagtgctcatatcaatccagtctcttatggacgagaaatccctatcacaacgaaccccgggctttaagcaggaagagacactct
gggcgatagcaagaactacaatggagtgcattccaacacgaagaccatttcgggtaacaatggtgtgtaaaatgctggaaggggcaaatgccaatt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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