XB-CLONE-4358021
Clone Name: IMAGE:7028351
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Gene:
fbxl15
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC084522 [+]
CGAGGTCATTTCTTTCCATACATGGTTTCCTTTCAAACAGGAATCAGGAAGGATAATAGATTCTGACATTGATTGTGACCTTGAAAATGGCTAAGGATGA
GGACAACAGCAGTGTCCATTTGTTGGATCTCCCTTGGGAAGATGTGTTGGTCCCACATATACTCCGCAATCTCCCACTGAGGCACATACTAAGTCTTCAG
AGAGTCAGCAAACCATTTCAGTCCCTGGTCCACCTTTATCTTGCCAACTGCCGCCATTTTGATTCTACTCAGTTGGGATCACAATTACCCAAAAGCACCT
TCTCCGAACTGCTTAGGAACAATACTGTATTGCAGAAACTTGACCTACAGAGCTGTTGTGATTGGTTAACGGACAAGGAGCTGCTTCCTGTCATTGGCCA
AAACCATCACCTCATCCACATCAACTTGAATAGCTGCGGACAGCTAACCCGGCAGTCATTGGTTGCAATATCGCTGAGCTGTCCTCACCTGCAGAACATT
TGCCTTGGTCATTGTGAGTGGGTGGACTGTCTTTCCCTGCGCAGTTTAGTTGACCATTGCAAATGTTTGGAGGCAATTGATCTGACAGCTTGTCGTCAGT
TGAAGGACGACACCATCAGCTACTTGGTGCAGAAGAGCACAAGATTAAAATCGCTTTCACTGGCAGTCAATGCAAACATTAGCGACATTGCTGTTGAAGA
AACAGCTAAGAACTGCAGAGATCTGGAACACCTCGATTTAACAGGATGCCTGCGTGTGAAAAATGATTCTATTAGGACCCTGGCTGAATACTGTACCAAA
CTAAAGTCCCTGAAAGTAAAGCACTGCCACAATGTCACGGAATCCAGCCTAGGAAACCTACGGAAGAGAGAAGTAGTGCTCGATGTGGAGCCTCCGCTGC
AGCGAGCATTGGTTCTCCTGCAGGATGTTGTGGGCTTTGGGCCATTTATAAATCTCCAGATCTAGAGCTGCTCCTATTACATCATACCTTTAGCTACCAT
TTGTTGTGCTGACTGCGGTGGAATATACATTCTTTTAAAAGGTGCTGTGAGGACAGAGAATACTGTATTACTTTTTGGCTTGCAGTAAACTGCCCTCATT
CAACTGGACCATCATCGTGAATTGTTTTTAATTGCTCCTTGGGATCCAATTCTTGAAACAGATCCACGAGCCAAGTGTTATAGTGAATGATATGATTCTG
TACAACATAATATAGACTATTTAATGTAATTAGTAAAATATGATATTCTGTATCATACAGTATGGAAATGTTTTAATTTAAATATGCAACTAACTATTGG
ACTCAACTTAACCAGAAGTATTTGTGTTTAGGGATTCTGGAACATAACAATACTTTGGCAGCCAAAATCACACCTATCATTAAAGTGTTTTTGCCAGAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CF781174 [+]taattaatccccccccccccccgaggtcatttctttccatacatggtttcctttcaaacaggaatcaggaaggataatagattctgacattgattgtgac
cttgaaaatggctaaggatgaggacaacagcagtgtccatttgttggatctcccttgggaagatgtgttggtcccacatatactccgcaatctcccactg
aggcacatactaagtcttcagagagtcagcaaaccatttcagtccctggtccacctttatcttgccaactgccgccattttgattctactcagttgggat
cacaattacccaaaagcaccttctccgaactgcttaggaacaatactgtattgcagaaacttgacctacagagctgttgtgattggttaacggacaagga
gctgcttcctgtcattggccaaaaccatcacctcatccacatcaacttgaatagctgcggacagctaacccggcagtcattggttgcaatatcgctgagc
tgtcctcacctgcagaacatttgccttggtcattgtgagtgggtggactgtctttccctgcgcagtttagttgaccattgcaaatgtttggaggcaattg
atctgacagcttgtcgtcagttgaaggacgacaccatcagctacttggtgccaaagagcacaagatttaaatcgctttcactggcagttcatgcaaacat
taccgaacttgctgttgaaaaaacagctaaaaactgcagaaatctggaacacttcgatttaaccaggaggcctgcctgtggaaaatgatttccattagga
accc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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