XB-CLONE-4358005
Clone Name: IMAGE:7028342
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Gene:
mmp1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080175
[+]
TAGCACCGGCAAATCCTAGAAAGCCTTAGAAGGATGAAGAGCCTCCTGCTGAAGCTGCTACTGTGTGTAGCCCTCACCGCCGCCTTCCCTACAGATAAAC
AAGATGCACCCACAGGAACCAGTGAGGAGATGGCTGAGAACTACTTGAAGAAATTCTACAGTCTCGGCACCGAGGGGGGACCAGTTGGAAGAAAGAAAAA
CAACCGTCCTTTCACTGAAAAGCTCCAGCAGATGCAGAAGTTTTTTGGCTTAAAGGTGACGGGGATACTGGACAGTAAGACAATAGAAGTCATGCAGAAA
CCCAGGTGTGGAGTCTATGATGTTGGGCAGTACAGCACCGTCCCAAAAAGTTCTGCATGGCAGAAAACTGATCTGACCTACAGAATCATAAACTTCACTC
CTGACCTGCCCCAGGCCGACGTGGAGGCTGCCATTCAAAGAGCTTTTAAAGTCTGGAGTGATGTGACACCTTTGACCTTCACCAGAATCTACAATGAAGT
ATCAGATATAGAAATCTCATTTTCAGCTGGAGATCACAAAGACAATTCTCCTTTTGATGGACCTGGTGGCATTTTAGCCCACGCCTTTCAGCCCGGCAAC
GGCATTGGTGGAGATGCTCATTTTGACGAAGATGAATCCTGGACCAAGACCAGCCAATTGTACAATCTGTTTCTTGTTGCTGCCCATGAATTCGGACACT
CCTTGGGGCTTTCTCATTCCACTGATCCAGGTGCCTTGATGTATCCATCTTACTCAAGTACCGACCCCAATGCATTTCAGCTTCCTCAGGATGATATAAA
TGCTATACAGTATCTATATGGAAAGTCTTCCAATCCAGTCCAACCAACCGGCCCAACCACTCCTACCATATGTGATCCCAACGTGGTTTTCGATGCTGTC
ACCACCTTGAGAGGAGAACTGATTTTCTTTCTTAACAGGTTTATATGGAGAAAGCATCCCCAAGCATCCGAGGCTGAACTCATGTTCGTGCAAACATTCT
GGCCATCACTGCCCAACGATATCGATGCTGCCTATGAAAATCCTATAACGGAGCAGATCCTTGTGTTTAAAGGAGCAAAATATACAGCTCTCAACGGCTT
CGATGTATTGCCAGGCTACCCCAGGAACATCTACAGCCTGGGATTCCCAAAGACCGTGAAGAGAATCGACGCGGCTGTTCATATTGAGCACCTCGCGAAA
ACATATTTCTTTGTGGCTAATAAATATTGGAGTTATGATGAAGATAAACAGCAAATGGACAAAGGCTTTCCAAAACTAATAAGAGACGATTTCCCAGGAA
TCCCTGAGAAAATTAATGCAGCCCTTTATTATAGAGGTCGCCTGTATTTCTTCATTGGACGGGTCCAGTTTGAATACAACCTCAACACTAAGAAAATTGT
ACAGGTTTTGAGAAGCAACAGCTGGTTGGGCTGCTAACACCACAGACTCCCTGCTCTCTCACCACAGACTCCCTGCTCTCTCACCACAGACTCCCTGCTC
TCTCACCACAGACTCCCTGCTCTCTAATAGAGGACAATATTGACAGCGACTGTTGCTCATTTCTGCAGCTGTCAGTAGTTTCCATATCATTTCAATTCAA
TGCAACTTTTGGGTTTATTTAATCATTATTAATTAATATTTTATACTACTTTTTCTTTAATAAATATTTTTTGTTTAAAAAAAAAAAAAAAA
5' EST: CF781182
[+]taattatccccccccccccctagcaccggcaaatcctagaaagccttagaaggatgaagagcctcctgctgaagctgctactgtgtgtagccctcaccgc
cgccttccctacagataaacaagatgcacccacaggaaccagtgaggagatggctgagaactacttgaagaaattctacagtctcggcaccgagggggga
ccagttggaagaaagaaaaacaaccgtcctttcactgaaaagctccagcagatgcagaagttttttggcttaaaggtgacggggatactggacagtaaga
caatataagtcatgcagaaacccaggtgtggagtctatgatgttgggcaggacagcaccgtcccgaaaagttctgcatggcagaaaactgatctgaccta
ctgaatcataaacttcactcctgacctggaccaagcccaacgtggaaggctgccattcaaagaagcttttaaagggctgggaagggatggatgaaaaccc
tttgaacccattccccaaaaaacctagaaaatggaaatttgtccgggatgtttggggtatgccgcaattttttacaacgcttgagataaagtgagagaga
aaaggacaagctgaagatcgcctctttttggaaaagtggaccacctatatgggttgggggagacaatttttgttgtaaagagggtaccctgagagatggt
agcgagtcatttgaaggtcagatcgcncataataacattaccactggggtcatattttttgtctgtgtggggagataaaaaaagtaagcatcgacgaaat
atttttcgcgcgccgtggtgtattgtaacttcacacatgtgcacccgtgggggacaaacacccaagaaaacacccgcaaccccaccatataatttagtta
ttttaaccaaaatagtagggtgtttttcctcttggtttttggtgtcgtgactacctctatatgaaaaatatttgtgaggtgaaattatattatgcggcta
tagggtgcgccatcatgcattgctgagacggtgcacccactcaattagattcccacaaatgaggtacctctcccagcgaataaggtattcacagcggttc
tgggatatatgagggggggtattagagagcgaggagcatgataaaatagaggcgacatgtctac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: