XB-CLONE-4357935
Clone Name: IMAGE:7028671
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Gene:
amacr
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080155 [+]
GAGTTCAGCCCAGACAGTGAGTTGGGGGTACTGCTGGCAGCTCTAATAGATTTACATGAATAAGAGGAATGGCTTTAGCTGGGGTTCGTGTTCTGGAACT
GGCAGGTCTTGCTCCTGCGCCATTTTGCGGAATGATTCTAGCAGATTTTGGTGCCAAAGTGATCCGAGTGGATAAAAGTAGTAGCAATGTAATAATGGAC
ACCATGGCACGAGGAAAAAAATCTATAGTTGTGAACCTCAAGAACCGGGAAGGGATCAGTGTATTGAAGAAATTATGCAAAAAGTCTGATGTCTTGATTG
AGCCTTTCCGTCATGGTGTCATGGAGAGCCTTGGGCTCGGACCCGATATTATGCTTAAAGAAAACCCACAGCTTATTTACGCAAGACTCACTGGATTTGG
CCAGAGTGGAAAATATGCAAAATCAGCTGGTCATGACATCAATTATGTCTCAATGTCAGGTTTGCTGTCAAAACTGGGAGTCAAAAATGACAGTCCAACC
CCACCTCTTAACCTTCTGGCAGACTTCGCTGGTGGTGGTCTGATATGTGCATTGGGAATTGTTATGTCGCTGTATGAGCGCACTATGTCAGGCAAAGGCC
AAGTCATTGATTGTAGTATGGTGGAAGGCGCTGCATATGTTGGATCCTTTATTTGGAAGAGCCAGAAACTGGGCCTGTGGAGTAATTCTCCAGGTGAAAA
TTTGCTGGATGGGGGAGCTCCTTTCTATACCACATACATGACGGCAGATGGAAAATTTATGGCAGTGGGCCCTCTTGAAACCAAGTTCTATGCCGAATTG
TTAAAAGGACTTGAGCTTGATGCCTCTGAATTACCTCATCAGATGAGCTTCTCGGACTGGCCCGAAATGAAGAGAATCTTTGCCCAAAAGTTCCGAGAGA
AAACACAGGCAGAATGGTGCCGAATATTTGATGGAACCGATGCTTGTGTTACTCCAGTCCTCAGCTTTAGTGACGTTGCCTTACACCAACACAATGAGGA
TCGTGGATCTTTTATATGCAACGATGAAGGGGAATTAAGCCCACGGCCTGCTCCTCTTCTCTCTAGAACACCTGCAGCTCCAAACACCAGCAGGGATCCT
TTGGTCGGAGAACACACGCATGAGATACTCGCAGAATATGGCTTCAGCCAAAGGGAAATTTCTGATCTCCAAACCCGCGGAGCAGTTGCAACAAACAAAG
CCAAAGCTCAGTTGTAAAGAACAGTATCAAGGTTAACAATGCAAGCTGAAATTGTGATATCAAAAATTACTGATTGCCGACAATCAGCATCTAATTCATT
ACTGGATTTTGTTCTATAATGTGTCCAAAGACATAAGAGAAGGCTGTGGTGTTTGGTATGCAAGATTTAAATATTCTGTGTTTTAATGGGATATTGTAAC
AATAATATTTAATTGCAAAATTAAGATGAACAAAGGAAAGAAAATAAAAGTGAATGAACAAAAAAAAAAAAAAAA
5' EST: CF781217 [+]taattaatcccccccccccccgagttcagcccagacagtgagttgggggtactgctggcagctctaatagatttacatgaataagaggaatggctttagc
tggggttcgtgttctggaactggcaggtcttgctcctgcgccattttgcggaatgattctagcagattttggtgccaaagtgatccgagtggataaaagt
agtagcaatgtaataatggacaccatggcacgaggaaaaaaatctatagttgtgaacctcaagaaccgggaagggatcagtgtattgaagaaattatgca
aaaagtctgatgtcttgattgagcctttccgtcatggtgtcatggagagccttgggctcggacccgatattatgcttaaagaaaacccacagcttattta
cgcaagactcactggatttggccagagtggaaaatatgcaaaatcagctggtcatgacatcaattatgtctcaatgtcaggtttgctgtcaaaactggga
gtcaaaaatgacagtccaaccccacctcttaaccttctggcagacttcngctggtggtggtctgatatgtgcattgggnaattgtttatgtcgctgtatg
agcgcactatgtcaggcaaaggccaagtcattgattgtagnaaaggtgggaaggcgctgcataatgttggatccttttatttgggagagccagaaactgg
gccctgtgggagtaattctccaagtgnaaaattttgctgggatggggggacctccttttctataccacatacatgacgggcagagggaaaaattttatgg
cagtggggccctcttggaaaacaagtttct
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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