XB-CLONE-4357729
Clone Name: IMAGE:7028489
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Gene:
atg4c
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080152 [+]
AGAAGAGACCATGGCAGAGCGCCGCACATTTACTCAAAAGACAAGCGGGTAAAGTGAATGATAAAGGCATGGGTTTGCTTTCCAGACATAAATATGGAAG
CCAGTGGCACAGATGATGTGGAGAAATTAAAATCCAAGTTCTTATCTGCATGGCATAACATGAAATATAGTTGGGTACTGAAAACAAAAACCTACTTCAA
GAGAAATTCACCAGTATTCTTACTTGGCAAATGCTACCATTTTAAATATGAAGATTCTAGTGTAACATCTGATGGAGGTTCCAATTCTGGCTCTGAATCA
AAAGAAGACCTTTCTGGAAATGTGGATGAATTTCGGAAGGATTTCATTTCCAGAATATGGCTTACTTATAGAGAAGAATTCCCCCAAATAGAAACATCGT
CATGGACAACAGATTGTGGATGGGGCTGCACATTACGTACTGGTCAAATGTTGCTGGCACAGGGATTGATAGTACACTTCCTGGGACGAGACTGGACTTG
GACAGAAGCACTGGACATTTTCTCTTCTGAATCGGAATTTTGGACAGCGAACACTGCAAGAAAACTTACTCCATCTCTTGAGACATCTTTCTCTGAAAAT
AATGAATGTGTGTCATCAAACAAGCAGCCATTGCACAACTGTGATAAGAAAAGTAATTCTGAGGACTTTCACCAAAAAATAATATCCTGGTTTGCAGACT
ACCCACTGGCCTATTTTGGTTTACATCAACTTGTAAAGCTTGGGAAAAATTCGGGCAAAGTGGCTGGGGACTGGTATGGTCCAGCAGTTGTTTCTCATCT
TTTGAGGAAAGCGATTGAAGAGAGCTCAGATCCAGAACTGCAGGGAATAACAATTTATGTTGCTCAGGACTGCACTATCTACAGTGCTGATGTCTATGAC
CTGCAGTGTAATAAGGGTACCGAGAAGGCTGTTGTAATTCTAGTTCCTGTGAGACTTGGGGGTGAAAGGACCAATATGGAATATTTTGAGTTTGTAAAGG
GAATTCTAAGCCTTGAATTCTGTATTGGAATTATCGGTGGCAAACCCAAGCAGTCCTATTACTTTGTTGGATTTCAAGATGACAGTTTGATTTACATGGA
TCCTCATTACTGCCAATCTTTTGTTGATGTCAGCGTAAAGAATTTCCCTCTTGAGTCTTTCCATTGCCCTTCTCCTAAGAAGATGTCTTTCAAGAAAATG
GATCCCAGTTGCACAATTGGGTTTTACTGTAGGAATGCTCGAGAATTTGAAAAGGCAGCGGAAGAACTTACAAAGGTGCTGAAATCTTCAACCAAGCAGA
ATTATCCTTTATTTACCTTTGTAAATGGACATGCTCAAGACTTTGATTTTGTCTGTACTCCAGTCTACGATCAAAATGACCTTTTCACAGAGGATGAAAA
GAAAAGATTAAAAAGATTTAGCACAGAGGAGTTTGTCTTGCTCTAAAGACATTTTTGCACTCCTGGCAGCAGTCCATAGCACATTCATTCTAACAAAAAC
ACAAGGAAAAGAACACTAGACTTTTTAGACTTTTTATATGTTTTTAAATTAAAATTTTAAAGTTTTTAATGCAACGGAAAACTTTTTTTTAAATGTGTTT
GTAATTTTGTAATATTGTTGTACTAATAACCATATAATGATGCACGGGAAGGAGTCTGCTTTGCAAATTGGAGCAAATGCACGATTTAATCCTCTCCTGT
GTGTAAAAGGTACAGTATGGTGCAAATTTACTTTTGGACTGTAAACAACCTTATTTAAACAGTTTTTTTGGGGTTTTTTTGTTTCCATTTTTTTCTGTTT
CCTCCTACCTTTTGTGCAGGGAAATTAGGACAAGATGGATCTGCCATTTTGCCAGTGAAGTGGGGGCAAGATACTAGTTTTACATGGGGTCTCCTTGGTG
AAAAAAAAAAAAAAA
5' EST: CF781320 [+]taattaatcccccccccccccagaagagaccatggcagagcgccgcacatttactcaaaagacaagcgggtaaagtgaatgataaaggcatgggtttgct
ttccagacataaatatggaagccagtggcacagatgatgtggagaaattaaaatccaagttcttatctgcatggcataacatgaaatatagttgggtact
gaaaactttaacctacttcaagagaaattcaccagtattcttacttggcaaatgctaccattttaaatatgaagattctagtgtaacatctgatggaggt
tccaattctggctctgaatcaaaagaagacctttctggaaatgtggatgaatttcggaaggatttcatttccagaatatggcttacttatagagaagaat
tcccccaaatagaaacatcgtcatggacaacagattgtggatggggctgcacattacgtactggtcaaatgttgctggcacagggattgatagtacactt
cctgggacgagactggacttggacagaagcactggacattttctcttctgaatcggaattttggacagcgaacactgcaagaaaacttactccatctctt
gagacatctttctctgaaaataatgaatgtgtgtcatcaaacaaacagccattgcacaactgtgataagaaaagtattctgaggactttcaccaaaaaat
aatatcctggtttgcagactaaccactggccctattttggtttaactcaacttggaaaagcttgggaaaaaatccgggcaaagtgggctgggggactggg
tatgggtccaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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